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Chromatography, Gel

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Stability of infliximab solutions in different temperature and dilution conditions.

Journal of pharmaceutical and biomedical analysis
Infliximab is a monoclonal antibody widely used for the treatment of inflammatory diseases. Over the past few years, many studies have assessed that monoclonal antibodies are prone to aggregation under stress conditions. The aim of this study was to ...

Extraction optimization, purification and characterization of polysaccharides from the seed coat of black soybean.

PloS one
In this study, the extraction of water-soluble polysaccharides from the seed coat of black soybean (BSCP) was investigated and optimized. A response surface methodology based on a Box-Behnken design (BBD) was used to optimize the extraction condition...

[GPC Fingerprint Chromatograms of Aloe vera Leaf Gel Polysaccharides].

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
OBJECTIVE: To establish the gel permeation chromatography (GPC) fingerprint chromatograms of polysaccharides in Aloe vera leaf gel from the same habitat (Beijing) and different habitats for evaluating the quality of Aloe vera leaf gel products commer...

Determination of residual dextran sulfate in protein products by SEC-HPLC.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Dextran sulfate is a polyanionic derivative of dextran, produced by esterification of dextran with chlorosulphonic acid. Dextran sulfate with an average molecular weight of 8000Da can be added to the cell culture to inhibit binding of proteins to cel...

A robust robotic high-throughput antibody purification platform.

Journal of chromatography. A
Monoclonal antibodies (mAbs) have become the fastest growing segment in the drug market with annual sales of more than 40 billion US$ in 2013. The selection of lead candidate molecules involves the generation of large repertoires of antibodies from w...

PCprophet: a framework for protein complex prediction and differential analysis using proteomic data.

Nature methods
Despite the availability of methods for analyzing protein complexes, systematic analysis of complexes under multiple conditions remains challenging. Approaches based on biochemical fractionation of intact, native complexes and correlation of protein ...

An automated, low volume, and high-throughput analytical platform for aggregate quantitation from cell culture media.

Journal of chromatography. A
High throughput screening methods have driven a paradigm shift in biopharmaceutical development by reducing the costs of good manufactured (COGM) and accelerate the launch to market of novel drug products. Scale-down cell culture systems such as shak...