AIMC Topic: Escherichia coli

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Prediction of Protein-Protein Interactions with Local Weight-Sharing Mechanism in Deep Learning.

BioMed research international
Protein-protein interactions (PPIs) are important for almost all cellular processes, including metabolic cycles, DNA transcription and replication, and signaling cascades. The experimental methods for identifying PPIs are always time-consuming and ex...

DeLTA: Automated cell segmentation, tracking, and lineage reconstruction using deep learning.

PLoS computational biology
Microscopy image analysis is a major bottleneck in quantification of single-cell microscopy data, typically requiring human oversight and curation, which limit both accuracy and throughput. To address this, we developed a deep learning-based image an...

Machine Learning in Mass Spectrometry: A MALDI-TOF MS Approach to Phenotypic Antibacterial Screening.

Journal of medicinal chemistry
Machine learning techniques can be applied to MALDI-TOF mass spectral data of drug-treated cells to obtain classification models which assign the mechanism of action of drugs. Here, we present an example application of this concept to the screening o...

Design of (quinolin-4-ylthio)carboxylic acids as new Escherichia coli DNA gyrase B inhibitors: machine learning studies, molecular docking, synthesis and biological testing.

Computational biology and chemistry
Spread of multidrug-resistant Escherichia coli clinical isolates is a main problem in the treatment of infectious diseases. Therefore, the modern scientific approaches in decision this problem require not only a prevention strategy, but also the deve...

Genome-scale transcriptional dynamics and environmental biosensing.

Proceedings of the National Academy of Sciences of the United States of America
Genome-scale technologies have enabled mapping of the complex molecular networks that govern cellular behavior. An emerging theme in the analyses of these networks is that cells use many layers of regulatory feedback to constantly assess and precisel...

Covalent Functionalization of Graphene Sheets with Different Moieties and Their Effects on Biological Activities.

ACS biomaterials science & engineering
The ongoing spread of multi-drug-resistant bacteria over the past few decades necessitates collateral efforts to develop new classes of antibacterial agents with different mechanisms of action. The utilization of graphene nanosheets has recently gain...

Self-Assembled Polyurethane Capsules with Selective Antimicrobial Activity against Gram-Negative .

ACS biomaterials science & engineering
This article reports the antimicrobial activity of two segmented amphiphilic polyurethanes, PU-1 and PU-2, containing a primary or secondary amine group, respectively. In acidic water, intrachain H-bonding among the urethanes followed by hierarchical...

Synthesis and in vitro activity of asymmetric indole-based bisamidine compounds against Gram-positive and Gram-negative pathogens.

Bioorganic & medicinal chemistry letters
A series of new asymmetric bisamidine was designed, synthesized, and tested for their in-vitro antibacterial activity using a range of Gram-positive and Gram-negative pathogens. Most compounds demonstrated powerful antibacterial activity, and interes...

High-level expression of Thermobifida fusca glucose isomerase for high fructose corn syrup biosynthesis.

Enzyme and microbial technology
Glucose isomerase (GIase), an efficient enzyme in the isomerization of d-glucose to d-fructose, has been widely used in food processing. In this study, an efficient expression system for a Thermobifida fusca GIase (GIase) in Escherichia coli was firs...

Research Note: Longitudinal monitoring of chicken houses in a commercial layer farm for antimicrobial resistance in Escherichia coli with special reference to plasmid-mediated quinolone resistance.

Poultry science
Plasmid-mediated quinolone resistance (PMQR) genes located on conjugative plasmids can be transferred to other bacteria in the absence of antimicrobial selective pressure. To elucidate the prevalence of resistance, including PMQR in an egg-producing ...