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Molecular Sequence Annotation

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Using deep learning to annotate the protein universe.

Nature biotechnology
Understanding the relationship between amino acid sequence and protein function is a long-standing challenge with far-reaching scientific and translational implications. State-of-the-art alignment-based techniques cannot predict function for one-thir...

PFmulDL: a novel strategy enabling multi-class and multi-label protein function annotation by integrating diverse deep learning methods.

Computers in biology and medicine
Bioinformatic annotation of protein function is essential but extremely sophisticated, which asks for extensive efforts to develop effective prediction method. However, the existing methods tend to amplify the representativeness of the families with ...

SVPath: an accurate pipeline for predicting the pathogenicity of human exon structural variants.

Briefings in bioinformatics
Although there are a large number of structural variations in the chromosomes of each individual, there is a lack of more accurate methods for identifying clinical pathogenic variants. Here, we proposed SVPath, a machine learning-based method to pred...

TransformerGO: predicting protein-protein interactions by modelling the attention between sets of gene ontology terms.

Bioinformatics (Oxford, England)
MOTIVATION: Protein-protein interactions (PPIs) play a key role in diverse biological processes but only a small subset of the interactions has been experimentally identified. Additionally, high-throughput experimental techniques that detect PPIs are...

DeepPlnc: Bi-modal deep learning for highly accurate plant lncRNA discovery.

Genomics
We present here a bi-modal CNN based deep-learning system, DeepPlnc, to identify plant lncRNAs with high accuracy while using sequence and structural properties. Unlike most of the existing software, it works accurately even in conditions with ambigu...

Hierarchical deep learning for predicting GO annotations by integrating protein knowledge.

Bioinformatics (Oxford, England)
MOTIVATION: Experimental testing and manual curation are the most precise ways for assigning Gene Ontology (GO) terms describing protein functions. However, they are expensive, time-consuming and cannot cope with the exponential growth of data genera...

E-SNPs&GO: embedding of protein sequence and function improves the annotation of human pathogenic variants.

Bioinformatics (Oxford, England)
MOTIVATION: The advent of massive DNA sequencing technologies is producing a huge number of human single-nucleotide polymorphisms occurring in protein-coding regions and possibly changing their sequences. Discriminating harmful protein variations fro...

Elucidating the functional roles of prokaryotic proteins using big data and artificial intelligence.

FEMS microbiology reviews
Annotating protein sequences according to their biological functions is one of the key steps in understanding microbial diversity, metabolic potentials, and evolutionary histories. However, even in the best-studied prokaryotic genomes, not all protei...

Annotation of biologically relevant ligands in UniProtKB using ChEBI.

Bioinformatics (Oxford, England)
MOTIVATION: To provide high quality, computationally tractable annotation of binding sites for biologically relevant (cognate) ligands in UniProtKB using the chemical ontology ChEBI (Chemical Entities of Biological Interest), to better support effort...

CARD 2023: expanded curation, support for machine learning, and resistome prediction at the Comprehensive Antibiotic Resistance Database.

Nucleic acids research
The Comprehensive Antibiotic Resistance Database (CARD; card.mcmaster.ca) combines the Antibiotic Resistance Ontology (ARO) with curated AMR gene (ARG) sequences and resistance-conferring mutations to provide an informatics framework for annotation a...