AIMC Topic: Cell Count

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Comparison of physical and behavioral traits between dairy cows with low and high somatic cell count.

Preventive veterinary medicine
The objective of this study was to examine associations of locomotion score, hygiene, body condition score (BCS), lying behavior, and milk production with dairy cow somatic cell count (SCC; low or high). Cows from 14 commercial free-stall dairy herds...

Quantifying cell densities and biovolumes of phytoplankton communities and functional groups using scanning flow cytometry, machine learning and unsupervised clustering.

PloS one
Scanning flow cytometry (SFCM) is characterized by the measurement of time-resolved pulses of fluorescence and scattering, enabling the high-throughput quantification of phytoplankton morphology and pigmentation. Quantifying variation at the single c...

Analogous detection of circulating tumor cells using the AccuCyte -CyteFinder system and ISET system in patients with locally advanced and metastatic prostate cancer.

The Prostate
INTRODUCTION: Circulating tumor cells (CTCs) can provide important information on patient's prognosis and treatment efficacy. Currently, a plethora of methods is available for the detection of these rare cells. We compared the outcomes of two of thos...

Milking machine and udder health management factors associated with bulk milk somatic cell count in Uruguayan herds.

Preventive veterinary medicine
This paper describes the findings of static milking machine tests and milking observations on Uruguayan dairy farms. The aim of this study was to investigate the association between both milking machine performance and udder health management factors...

Construction of a system using a deep learning algorithm to count cell numbers in nanoliter wells for viable single-cell experiments.

Scientific reports
For single-cell experiments, it is important to accurately count the number of viable cells in a nanoliter well. We used a deep learning-based convolutional neural network (CNN) on a large amount of digital data obtained as microscopic images. The tr...

Impact of subclinical mastitis on greenhouse gas emissions intensity and profitability of dairy cows in Norway.

Preventive veterinary medicine
Impaired animal health causes both productivity and profitability losses on dairy farms, resulting in inefficient use of inputs and increase in greenhouse gas (GHG) emissions produced per unit of product (i.e. emissions intensity). Here, we used subc...

Automating cell detection and classification in human brain fluorescent microscopy images using dictionary learning and sparse coding.

Journal of neuroscience methods
BACKGROUND: Immunofluorescence (IF) plays a major role in quantifying protein expression in situ and understanding cell function. It is widely applied in assessing disease mechanisms and in drug discovery research. Automation of IF analysis can trans...

Influence of milking method, disinfection and herd management practices on bulk tank milk somatic cell counts in tropical dairy herds in Colombia.

Veterinary journal (London, England : 1997)
The aims of this study were to evaluate the effects of milking method, disinfection practices and other management factors on the bulk tank milk somatic cell count (BTSCC) in tropical dairy herds and to examine potential interactions with time. One h...

Multiparameter mechanical and morphometric screening of cells.

Scientific reports
We introduce a label-free method to rapidly phenotype and classify cells purely based on physical properties. We extract 15 biophysical parameters from cells as they deform in a microfluidic stretching flow field via high-speed microscopy and apply m...

GC-MS Metabolomics Identifies Metabolite Alterations That Precede Subclinical Mastitis in the Blood of Transition Dairy Cows.

Journal of proteome research
The objectives of this study were to determine alterations in the serum metabolites related to amino acid (AA), carbohydrate, and lipid metabolism in transition dairy cows before diagnosis of subclinical mastitis (SCM), during, and after diagnosis of...