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Cell Count

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Impact of subclinical mastitis on greenhouse gas emissions intensity and profitability of dairy cows in Norway.

Preventive veterinary medicine
Impaired animal health causes both productivity and profitability losses on dairy farms, resulting in inefficient use of inputs and increase in greenhouse gas (GHG) emissions produced per unit of product (i.e. emissions intensity). Here, we used subc...

Milking machine and udder health management factors associated with bulk milk somatic cell count in Uruguayan herds.

Preventive veterinary medicine
This paper describes the findings of static milking machine tests and milking observations on Uruguayan dairy farms. The aim of this study was to investigate the association between both milking machine performance and udder health management factors...

Analogous detection of circulating tumor cells using the AccuCyte -CyteFinder system and ISET system in patients with locally advanced and metastatic prostate cancer.

The Prostate
INTRODUCTION: Circulating tumor cells (CTCs) can provide important information on patient's prognosis and treatment efficacy. Currently, a plethora of methods is available for the detection of these rare cells. We compared the outcomes of two of thos...

Influence of milking method, disinfection and herd management practices on bulk tank milk somatic cell counts in tropical dairy herds in Colombia.

Veterinary journal (London, England : 1997)
The aims of this study were to evaluate the effects of milking method, disinfection practices and other management factors on the bulk tank milk somatic cell count (BTSCC) in tropical dairy herds and to examine potential interactions with time. One h...

GC-MS Metabolomics Identifies Metabolite Alterations That Precede Subclinical Mastitis in the Blood of Transition Dairy Cows.

Journal of proteome research
The objectives of this study were to determine alterations in the serum metabolites related to amino acid (AA), carbohydrate, and lipid metabolism in transition dairy cows before diagnosis of subclinical mastitis (SCM), during, and after diagnosis of...

Automating cell detection and classification in human brain fluorescent microscopy images using dictionary learning and sparse coding.

Journal of neuroscience methods
BACKGROUND: Immunofluorescence (IF) plays a major role in quantifying protein expression in situ and understanding cell function. It is widely applied in assessing disease mechanisms and in drug discovery research. Automation of IF analysis can trans...

Construction of a system using a deep learning algorithm to count cell numbers in nanoliter wells for viable single-cell experiments.

Scientific reports
For single-cell experiments, it is important to accurately count the number of viable cells in a nanoliter well. We used a deep learning-based convolutional neural network (CNN) on a large amount of digital data obtained as microscopic images. The tr...

Quantifying cell densities and biovolumes of phytoplankton communities and functional groups using scanning flow cytometry, machine learning and unsupervised clustering.

PloS one
Scanning flow cytometry (SFCM) is characterized by the measurement of time-resolved pulses of fluorescence and scattering, enabling the high-throughput quantification of phytoplankton morphology and pigmentation. Quantifying variation at the single c...

GPU-based deep convolutional neural network for tomographic phase microscopy with ℓ1 fitting and regularization.

Journal of biomedical optics
Tomographic phase microscopy (TPM) is a unique imaging modality to measure the three-dimensional refractive index distribution of transparent and semitransparent samples. However, the requirement of the dense sampling in a large range of incident ang...

U-Net: deep learning for cell counting, detection, and morphometry.

Nature methods
U-Net is a generic deep-learning solution for frequently occurring quantification tasks such as cell detection and shape measurements in biomedical image data. We present an ImageJ plugin that enables non-machine-learning experts to analyze their dat...