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In Situ Hybridization, Fluorescence

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Combined effects of volume ratio and nitrate recycling ratio on nutrient removal, sludge characteristic and microbial evolution for DPR optimization.

Journal of environmental sciences (China)
The optimization of volume ratio (V/V/V) and nitrate recycling ratio (R) in a two-sludge denitrifying phosphorus removal (DPR) process of Anaerobic Anoxic Oxic-Moving Bed Biofilm Reactor (A/O-MBBR) was investigated. The results showed that prolonged ...

Development and application of Peptide Nucleic Acid Fluorescence in situ Hybridization for the specific detection of Listeria monocytogenes.

Food microbiology
Listeria monocytogenes is one of the most important foodborne pathogens due to the high hospitalization and mortality rates associated to an outbreak. Several new molecular methods that accelerate the identification of L. monocytogenes have been deve...

[Feasibility Study for Storage and Re-Utilization of Circulating Tumor Cells (CTCs)].

Rinsho byori. The Japanese journal of clinical pathology
BACKGROUND: In this era of precision medicine, monitoring patients requires not only real time but also longitudinal sequence of samples at various time points. Based on this background, we focused on conditioned circumstances on fixation and storage...

Artificial Intelligence for the Evaluation of Operational Parameters Influencing Nitrification and Nitrifiers in an Activated Sludge Process.

Microbial ecology
Nitrification at a full-scale activated sludge plant treating municipal wastewater was monitored over a period of 237 days. A combination of fluorescent in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (qPCR) were use...

De novo prediction of human chromosome structures: Epigenetic marking patterns encode genome architecture.

Proceedings of the National Academy of Sciences of the United States of America
Inside the cell nucleus, genomes fold into organized structures that are characteristic of cell type. Here, we show that this chromatin architecture can be predicted de novo using epigenetic data derived from chromatin immunoprecipitation-sequencing ...

Semantic segmentation of mFISH images using convolutional networks.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
Multicolor in situ hybridization (mFISH) is a karyotyping technique used to detect major chromosomal alterations using fluorescent probes and imaging techniques. Manual interpretation of mFISH images is a time consuming step that can be automated usi...

Artificial intelligence to detect MYC translocation in slides of diffuse large B-cell lymphoma.

Virchows Archiv : an international journal of pathology
In patients with suspected lymphoma, the tissue biopsy provides lymphoma confirmation, classification, and prognostic factors, including genetic changes. We developed a deep learning algorithm to detect MYC rearrangement in scanned histological slide...

The Use of Quantitative Digital Pathology to Measure Proteoglycan and Glycosaminoglycan Expression and Accumulation in Healthy and Diseased Tissues.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Advances in reagents, methodologies, analytic platforms, and tools have resulted in a dramatic transformation of the research pathology laboratory. These advances have increased our ability to efficiently generate substantial volumes of data on the e...