Infra-species taxonomy is a prerequisite to compare features such as virulence in different pathogen lineages. Mycobacterium tuberculosis complex taxonomy has rapidly evolved in the last 20 years through intensive clinical isolation, advances in sequ...
Polymerase chain reaction (PCR) is hailed as one of the monumental scientific techniques of the twentieth century, and has become a common and often indispensable technique in many areas. However, researchers still frequently find some DNA templates ...
Primers plays important role in polymerase chain reaction (PCR) experiments, thus it is necessary to select characteristic primers. Unfortunately, manual primer design manners are time-consuming and easy to get human negligence because many PCR const...
Molecular assays are critical tools for the diagnosis of infectious diseases. These assays have been extremely valuable during the COVID pandemic, used to guide both patient management and infection control strategies. Sustained transmission and unhi...
As pathogen molecular diagnostics technology develops, the software analysis of polymerase chain reaction sigmoidal curves, cycle threshold (Ct) values, and the limit of nucleic acid detection for that assay is not always available when reporting tes...
Polymerase chain reaction (PCR) inspection of salivary analyte is performed by pretreatment, RNA extraction setup, RNA extraction, PCR setup, and the PCR process. However, the pretreatment process is conducted manually, and it is a bottleneck to the ...
European review for medical and pharmacological sciences
Nov 1, 2020
Recent Coronavirus (COVID-19) is one of the respiratory diseases, and it is known as fast infectious ability. This dissemination can be decelerated by diagnosing and quarantining patients with COVID-19 at early stages, thereby saving numerous lives. ...
Transactions of the Royal Society of Tropical Medicine and Hygiene
Dec 1, 2017
BACKGROUND: In order to investigate the occurrence of Giardia duodenalis and its genotypes in vegetables that are consumed raw, we analyzed samples cultivated with organic or chemical fertilizer, sold in street markets and from community vegetable ga...
Revista Brasileira de terapia intensiva
Jan 1, 2017
OBJECTIVES: To define the frequency of cytomegalovirus disease among kidney transplant patients in an intensive care unit in which this complication was suspected and to identify predisposing factors and their possible impact on clinical outcome.
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