AIMC Topic: Polymerase Chain Reaction

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Prediction of success for polymerase chain reactions using the Markov maximal order model and support vector machine.

Journal of theoretical biology
Polymerase chain reaction (PCR) is hailed as one of the monumental scientific techniques of the twentieth century, and has become a common and often indispensable technique in many areas. However, researchers still frequently find some DNA templates ...

Estimation of teaching-learning-based optimization primer design using regression analysis for different melting temperature calculations.

IEEE transactions on nanobioscience
Primers plays important role in polymerase chain reaction (PCR) experiments, thus it is necessary to select characteristic primers. Unfortunately, manual primer design manners are time-consuming and easy to get human negligence because many PCR const...

Using machine learning models to predict the impact of template mismatches on polymerase chain reaction assay performance.

Scientific reports
Molecular assays are critical tools for the diagnosis of infectious diseases. These assays have been extremely valuable during the COVID pandemic, used to guide both patient management and infection control strategies. Sustained transmission and unhi...

At the Mercy of the Artificial Intelligence Algorithm: Discrepant Polymerase Chain Reaction Results.

The Journal of infectious diseases
As pathogen molecular diagnostics technology develops, the software analysis of polymerase chain reaction sigmoidal curves, cycle threshold (Ct) values, and the limit of nucleic acid detection for that assay is not always available when reporting tes...

Research on automatic preprocessing equipment of salivary analyte for PCR inspection of coronavirus.

Science progress
Polymerase chain reaction (PCR) inspection of salivary analyte is performed by pretreatment, RNA extraction setup, RNA extraction, PCR setup, and the PCR process. However, the pretreatment process is conducted manually, and it is a bottleneck to the ...

Deep learning applications to combat the dissemination of COVID-19 disease: a review.

European review for medical and pharmacological sciences
Recent Coronavirus (COVID-19) is one of the respiratory diseases, and it is known as fast infectious ability. This dissemination can be decelerated by diagnosing and quarantining patients with COVID-19 at early stages, thereby saving numerous lives. ...

Genotyping of Giardia duodenalis in vegetables cultivated with organic and chemical fertilizer from street markets and community vegetable gardens in a region of Southern Brazil.

Transactions of the Royal Society of Tropical Medicine and Hygiene
BACKGROUND: In order to investigate the occurrence of Giardia duodenalis and its genotypes in vegetables that are consumed raw, we analyzed samples cultivated with organic or chemical fertilizer, sold in street markets and from community vegetable ga...

Prevalence of cytomegalovirus disease in kidney transplant patients in an intensive care unit.

Revista Brasileira de terapia intensiva
OBJECTIVES: To define the frequency of cytomegalovirus disease among kidney transplant patients in an intensive care unit in which this complication was suspected and to identify predisposing factors and their possible impact on clinical outcome.

[Construction and Identification of the cDNA Expression Library for Human Esophageal Cancer Cells].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
OBJECTIVES: To construct a cDNA phage expression library for human esophageal cancer cells.