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Reverse Transcriptase Polymerase Chain Reaction

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Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses.

Frontiers in cellular and infection microbiology
INTRODUCTION: Viral calf diarrhea poses a significant challenge to the cattle industry worldwide due to its high morbidity and mortality rates, leading to substantial economic losses. The clinical symptoms associated with various diarrhea pathogens o...

ai-corona: Radiologist-assistant deep learning framework for COVID-19 diagnosis in chest CT scans.

PloS one
The development of medical assisting tools based on artificial intelligence advances is essential in the global fight against COVID-19 outbreak and the future of medical systems. In this study, we introduce ai-corona, a radiologist-assistant deep lea...

Fast automated detection of COVID-19 from medical images using convolutional neural networks.

Communications biology
Coronavirus disease 2019 (COVID-19) is a global pandemic posing significant health risks. The diagnostic test sensitivity of COVID-19 is limited due to irregularities in specimen handling. We propose a deep learning framework that identifies COVID-19...

A multipurpose machine learning approach to predict COVID-19 negative prognosis in São Paulo, Brazil.

Scientific reports
The new coronavirus disease (COVID-19) is a challenge for clinical decision-making and the effective allocation of healthcare resources. An accurate prognostic assessment is necessary to improve survival of patients, especially in developing countrie...

Machine learning is the key to diagnose COVID-19: a proof-of-concept study.

Scientific reports
The reverse transcription-polymerase chain reaction (RT-PCR) assay is the accepted standard for coronavirus disease 2019 (COVID-19) diagnosis. As any test, RT-PCR provides false negative results that can be rectified by clinicians by confronting clin...

The application of a deep learning system developed to reduce the time for RT-PCR in COVID-19 detection.

Scientific reports
Reducing the time to diagnose COVID-19 helps to manage insufficient isolation-bed resources and adequately accommodate critically ill patients. There is currently no alternative method to real-time reverse transcriptase polymerase chain reaction (RT-...

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots.

Journal of visualized experiments : JoVE
The emergence of the recent SARS-CoV-2 global health crisis introduced key challenges for epidemiological research and clinical testing. Characterized by a high rate of transmission and low mortality, the COVID-19 pandemic necessitated accurate and e...

Developing an interpretation model for body fluid identification.

Forensic science international
Criminal investigations, particularly sexual assaults, frequently require the identification of body fluid type in addition to body fluid donor to provide context. In most cases this can be achieved by conventional methods, however, in certain scenar...