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Spectrometry, Fluorescence

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Determination of monophenolase activity based on backpropagation neural network analysis of three-dimensional fluorescence spectroscopy.

Journal of biotechnology
Tyrosinase is pivotal for melanin formation. Measuring monophenolase activity is of great importance for both fundamental research and industrial applications. For the first time, a backpropagation (BP) artificial neural network with three-dimensiona...

Identification of liquors from the same brand based on ultraviolet, near-infrared and fluorescence spectroscopy combined with chemometrics.

Food chemistry
Accurate identification of various liquors from the same brand is of great significance for safeguarding the rights and interests of consumers and the market economy. Here, the spectral properties of liquors were studied based on ultraviolet (UV), ne...

A mitochondria-targeting nitroreductase fluorescent probe with large Stokes shift and long-wavelength emission for imaging hypoxic status in tumor cells.

Analytica chimica acta
Development of a mitochondria-targeting fluorescent probe with large Stokes shift and long-wavelength emission was benefit for accurate detection of hypoxic status, which was known as a major factor of the tumor physiology and influence important pat...

Fluorescent silicon nanoparticles inhibit the amyloid fibrillation of insulin.

Journal of materials chemistry. B
Amyloid fibrillation of proteins is likely a key factor leading to the development of amyloidosis-associated diseases. Inhibiting amyloid fibrillation has become a crucial therapeutic strategy. Water-soluble, fluorescent silicon nanoparticles (SiNPs)...

Near-infrared mito-specific fluorescent probe for ratiometric detection and imaging of alkaline phosphatase activity with high sensitivity.

Journal of materials chemistry. B
Fluorescent probe-based analytical methods for biological species have gained increasing attention for their powerful detection and imaging capabilities. However, it is still occasionally problematic for accurate analyses due to the influence of the ...

Molecular structure regulation and enzyme cascade signal amplification strategy for upconversion ratiometric luminescent and colorimetric alkaline phosphatase detection.

Analytica chimica acta
Herein, molecular structure regulation and enzyme cascade signals amplification (MRECAmp) strategy was proposed to construct a novel upconversion ratiometric fluorescence and colorimetric dual-readout assay platform for highly sensitive and selective...

Reduced methicillin-resistant Staphylococcus aureus biofilm formation in bone cavities by photodynamic therapy.

Photodiagnosis and photodynamic therapy
UNLABELLED: Photodynamic Therapy (PDT) is a promising alternative for the treatment of infectious bone lesions in the oral cavity. The objective of this study was to evaluate the antimicrobial effectiveness of PDT using blue LED associated with curcu...

Aptamer based fluorometric β-lactoglobulin assay based on the use of magnetic nanoparticles and carbon dots.

Mikrochimica acta
The authors describe a fluorometric aptamer based assay for detecting β-lactoglobulin by using carbon dots (C-dots) as a signal indicator. The aptamer was immoblized on magnetite (FeO) nanoparticles (MNPs), and the C-dots served as a label for the co...

Design of a calix[4]arene-functionalized metal-organic framework probe for highly sensitive and selective monitor of hippuric acid for indexing toluene exposure.

Analytica chimica acta
In the present work, a novel metal-organic framework (MOF) fluorescent probe was prepared by post-synthetic modification of MIL-53-NH(Al) with carboxylatocalix[4]arene (CC[4]A). The introduced CC[4]A could not only enhance the fluorescence performanc...

Integrating DNA structure switch with branched hairpins for the detection of uracil-DNA glycosylase activity and inhibitor screening.

Talanta
The detection of uracil-DNA glycosylase (UDG) activity is pivotal for its biochemical studies and the development of drugs for UDG-related diseases. Here, we explored an integrated DNA structure switch for high sensitive detection of UDG activity. Th...