AIMC Topic: Cell Culture Techniques
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Manipulating cellular microRNAs and analyzing high-dimensional gene expression data using machine learning workflows.
STAR protocols
Oct 23, 2021
MicroRNAs (miRNAs) are elements of the gene regulatory network and manipulating their abundance is essential toward elucidating their role in patho-physiological conditions. We present a detailed workflow that identifies important miRNAs using a mach...
Leveraging advances in immunopathology and artificial intelligence to analyze in vitro tumor models in composition and space.
Advanced drug delivery reviews
Sep 1, 2021
Cancer is the leading cause of death worldwide. Unfortunately, efforts to understand this disease are confounded by the complex, heterogenous tumor microenvironment (TME). Better understanding of the TME could lead to novel diagnostic, prognostic, an...
Development of a novel noninvasive quantitative method to monitor Siraitia grosvenorii cell growth and browning degree using an integrated computer-aided vision technology and machine learning.
Biotechnology and bioengineering
Jul 28, 2021
The rapid, accurate and noninvasive detection of biomass and plant cell browning can provide timely feedback on cell growth in plant cell culture. In this study, Siraitia grosvenorii suspension cells were taken as an example, a phenotype analysis pla...
Development of Human iPSC-Derived Functional Neuronal Networks on Laser-Fabricated 3D Scaffolds.
ACS applied materials & interfaces
Feb 9, 2021
Neural progenitor cells generated from human induced pluripotent stem cells (hiPSCs) are the forefront of ″brain-on-chip″ investigations. Viable and functional hiPSC-derived neuronal networks are shaping powerful models for evaluating the normal and...
Integrated functional neuronal network analysis of 3D silk-collagen scaffold-based mouse cortical culture.
STAR protocols
Jan 25, 2021
Bioengineered 3D tunable neuronal constructs are a versatile platform for studying neuronal network functions, offering numerous advantages over existing technologies and providing for the discovery of new biological insights. Functional neural netwo...
Application of convolutional neural network on early human embryo segmentation during in vitro fertilization.
Journal of cellular and molecular medicine
Jan 24, 2021
Selection of the best quality embryo is the key for a faithful implantation in in vitro fertilization (IVF) practice. However, the process of evaluating numerous images captured by time-lapse imaging (TLI) system is time-consuming and some important ...
Deep-learning-based multi-class segmentation for automated, non-invasive routine assessment of human pluripotent stem cell culture status.
Computers in biology and medicine
Dec 11, 2020
Human induced pluripotent stem cells (hiPSCs) are capable of differentiating into a variety of human tissue cells. They offer new opportunities for personalized medicine and drug screening. This requires large quantities of high quality hiPSCs, obtai...
A robust soft sensor to monitor 1,3-propanediol fermentation process by Clostridium butyricum based on artificial neural network.
Biotechnology and bioengineering
Aug 20, 2020
With the aggravation of environmental pollution and energy crisis, the sustainable microbial fermentation process of converting glycerol to 1,3-propanediol (1,3-PDO) has become an attractive alternative. However, the difficulty in the online measurem...
Intelligent image-based deformation-assisted cell sorting with molecular specificity.
Nature methods
May 25, 2020
Although label-free cell sorting is desirable for providing pristine cells for further analysis or use, current approaches lack molecular specificity and speed. Here, we combine real-time fluorescence and deformability cytometry with sorting based on...
Analysis of neurite length of hippocampal neurons cultured into 3D artificial network patterned microfluidic chips.
The International journal of neuroscience
Feb 28, 2020
The study aims to lay a foundational probe for the thorough application microfluidic chips in brain function research with microfluidic chips. Neuron slide culture is a common culture method , and the microfluidic chip with the artificial network pa...
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