AIMC Topic: Cells, Cultured

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Single-cell Raman spectroscopy as a novel platform for unveiling the heterogeneity of mesenchymal stem cells.

Talanta
Despite the significant potential of mesenchymal stem cells (MSC) therapy in clinical settings, challenges persist regarding the efficient detection of consistency and uniformity of MSC populations. Raman spectroscopy is a fast, convenient, and nonde...

Evaluation of the effect of cell freshness on pyrogen detection using a serum-free monocyte-activation test.

PloS one
Pyrogens cause shock symptoms when released into the bloodstream. They are classified into two main categories: endotoxins (lipopolysaccharides [LPS]) and non-endotoxin pyrogens. The monocyte activation test (MAT) is an in vitro assay to detect pyrog...

Effects of aqueous and ethanolic extracts of Chinese propolis on dental pulp stem cell viability, migration and cytokine expression.

PeerJ
BACKGROUND: Propolis is a natural substance produced by honeybees that has various biological properties including, anti-inflammatory, antioxidant and antimicrobial properties. Although previous studies have evaluated the antimicrobial effects of pro...

Functional MRGPRX2 expression on peripheral blood-derived human mast cells increases at low seeding density and is suppressed by interleukin-9 and fetal bovine serum.

Frontiers in immunology
Primary human mast cells (MC) obtained through culturing of blood-derived MC progenitors are the preferred model for the study of MRGPRX2- IgE-mediated MC activation. In order to assess the impact of culture conditions on functional MRGPRX2 express...

Effect of laser photobiomodulation combined with hydroxyapatite nanoparticles on the osteogenic differentiation of mesenchymal stem cells using artificial intelligence: An in vitro study.

PloS one
AIM: To evaluate in vitro the effect of laser photobiomodulation (PBM) combined or not with 30-nm hydroxyapatite nanoparticles (HANp), on the osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) by morphometric analysi...

Evaluating deep learning techniques for optimal neurons counting and characterization in complex neuronal cultures.

Medical & biological engineering & computing
The counting and characterization of neurons in primary cultures have long been areas of significant scientific interest due to their multifaceted applications, ranging from neuronal viability assessment to the study of neuronal development. Traditio...

Enhanced drug classification using machine learning with multiplexed cardiac contractility assays.

Pharmacological research
Cardiac screening of newly discovered drugs remains a longstanding challenge for the pharmaceutical industry. While therapeutic efficacy and cardiotoxicity are evaluated through preclinical biochemical and animal testing, 90 % of lead compounds fail ...

Real-time hardware emulation of neural cultures: A comparative study of in vitro, in silico and in duris silico models.

Neural networks : the official journal of the International Neural Network Society
Biological neural networks are well known for their capacity to process information with extremely low power consumption. Fields such as Artificial Intelligence, with high computational costs, are seeking for alternatives inspired in biological syste...

Aberrant migration features in primary skin fibroblasts of Huntington's disease patients hold potential for unraveling disease progression using an image based machine learning tool.

Computers in biology and medicine
Huntington's disease (HD) is a complex neurodegenerative disorder with considerable heterogeneity in clinical manifestations. While CAG repeat length is a known predictor of disease severity, this heterogeneity suggests the involvement of additional ...

Protocol for machine-learning-based 3D image analysis of nuclear envelope tubules in cultured cells.

STAR protocols
The nuclear envelope can form complex structures in physiological and pathological contexts. Current approaches to quantify nuclear envelope structures can be time-consuming or inaccurate. Here, we present a protocol to measure nuclear envelope tubul...