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Cells, Cultured

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Mechanism of TNF- α inducing apoptosis and autophagy of chondrocytes by activating NF- κ B signal pathway.

Cellular and molecular biology (Noisy-le-Grand, France)
This study aimed to explore the mechanism of apoptosis and autophagy of chondrocytes induced by tumor necrosis factor α (TNA-α) by activating the NF-κB signal pathway. For this purpose, 24 SD rats were selected for feeding. The knee cartilage was cut...

Effect of laser photobiomodulation combined with hydroxyapatite nanoparticles on the osteogenic differentiation of mesenchymal stem cells using artificial intelligence: An in vitro study.

PloS one
AIM: To evaluate in vitro the effect of laser photobiomodulation (PBM) combined or not with 30-nm hydroxyapatite nanoparticles (HANp), on the osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) by morphometric analysi...

Machine learning aided single cell image analysis improves understanding of morphometric heterogeneity of human mesenchymal stem cells.

Methods (San Diego, Calif.)
The multipotent stem cells of our body have been largely harnessed in biotherapeutics. However, as they are derived from multiple anatomical sources, from different tissues, human mesenchymal stem cells (hMSCs) are a heterogeneous population showing ...

Early Predicting Osteogenic Differentiation of Mesenchymal Stem Cells Based on Deep Learning Within One Day.

Annals of biomedical engineering
Osteogenic differentiation of mesenchymal stem cells (MSCs) is proposed to be critical for bone tissue engineering and regenerative medicine. However, the current approach for evaluating osteogenic differentiation mainly involves immunohistochemical ...

Machine learning approach for recognition and morphological analysis of isolated astrocytes in phase contrast microscopy.

Scientific reports
Astrocytes are glycolytically active cells in the central nervous system playing a crucial role in various brain processes from homeostasis to neurotransmission. Astrocytes possess a complex branched morphology, frequently examined by fluorescent mic...

Protocol for machine-learning-based 3D image analysis of nuclear envelope tubules in cultured cells.

STAR protocols
The nuclear envelope can form complex structures in physiological and pathological contexts. Current approaches to quantify nuclear envelope structures can be time-consuming or inaccurate. Here, we present a protocol to measure nuclear envelope tubul...

Real-time hardware emulation of neural cultures: A comparative study of in vitro, in silico and in duris silico models.

Neural networks : the official journal of the International Neural Network Society
Biological neural networks are well known for their capacity to process information with extremely low power consumption. Fields such as Artificial Intelligence, with high computational costs, are seeking for alternatives inspired in biological syste...

Aberrant migration features in primary skin fibroblasts of Huntington's disease patients hold potential for unraveling disease progression using an image based machine learning tool.

Computers in biology and medicine
Huntington's disease (HD) is a complex neurodegenerative disorder with considerable heterogeneity in clinical manifestations. While CAG repeat length is a known predictor of disease severity, this heterogeneity suggests the involvement of additional ...

Enhanced drug classification using machine learning with multiplexed cardiac contractility assays.

Pharmacological research
Cardiac screening of newly discovered drugs remains a longstanding challenge for the pharmaceutical industry. While therapeutic efficacy and cardiotoxicity are evaluated through preclinical biochemical and animal testing, 90 % of lead compounds fail ...

Evaluating deep learning techniques for optimal neurons counting and characterization in complex neuronal cultures.

Medical & biological engineering & computing
The counting and characterization of neurons in primary cultures have long been areas of significant scientific interest due to their multifaceted applications, ranging from neuronal viability assessment to the study of neuronal development. Traditio...