AIMC Topic: Micronucleus Tests
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A deep learning workflow for quantification of micronuclei in DNA damage studies in cultured cancer cell lines: A proof of principle investigation.
Computer methods and programs in biomedicine
Feb 26, 2023
The cytokinesis block micronucleus assay is widely used for measuring/scoring/counting micronuclei, a marker of genome instability in cultured and primary cells. Though a gold standard method, this is a laborious and time-consuming process with perso...
Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence.
Journal of visualized experiments : JoVE
Jan 27, 2023
The micronucleus (MN) assay is used worldwide by regulatory bodies to evaluate chemicals for genetic toxicity. The assay can be performed in two ways: by scoring MN in once-divided, cytokinesis-blocked binucleated cells or fully divided mononucleated...
A machine learning method for improving the accuracy of radiation biodosimetry by combining data from the dicentric chromosomes and micronucleus assays.
Scientific reports
Dec 6, 2022
A large-scale malicious or accidental radiological event can expose vast numbers of people to ionizing radiation. The dicentric chromosome (DCA) and cytokinesis-block micronucleus (CBMN) assays are well-established biodosimetry methods for estimating...
Prediction of Micronucleus Assay Outcome Using In Vivo Activity Data and Molecular Structure Features.
Applied biochemistry and biotechnology
Oct 20, 2021
In vivo micronucleus assay is the widely used genotoxic test to determine the extent of chromosomal aberrations caused by the chemicals in human beings, which plays a significant role in the drug discovery paradigm. To reduce the uncertainties of the...
Inter-laboratory automation of the in vitro micronucleus assay using imaging flow cytometry and deep learning.
Archives of toxicology
Jul 10, 2021
The in vitro micronucleus assay is a globally significant method for DNA damage quantification used for regulatory compound safety testing in addition to inter-individual monitoring of environmental, lifestyle and occupational factors. However, it re...
The in vitro micronucleus assay using imaging flow cytometry and deep learning.
NPJ systems biology and applications
May 18, 2021
The in vitro micronucleus (MN) assay is a well-established assay for quantification of DNA damage, and is required by regulatory bodies worldwide to screen chemicals for genetic toxicity. The MN assay is performed in two variations: scoring MN in cyt...
The 3D reconstructed skin micronucleus assay using imaging flow cytometry and deep learning: A proof-of-principle investigation.
Mutation research. Genetic toxicology and environmental mutagenesis
Jan 20, 2021
The reconstructed skin micronucleus (RSMN) assay was developed in 2006, as an in vitro alternative for genotoxicity evaluation of dermally applied chemicals or products. In the years since, significant progress has been made in the optimization of th...
Mutagenicity Tests
Mitomycin
Skin, Artificial
Image Processing, Computer-Assisted
False Positive Reactions
Predictive Value of Tests
Proof of Concept Study
Flow Cytometry
Micronucleus Tests
Software
Models, Biological
Humans
Skin
Automation, Laboratory
Tissue Scaffolds
Artificial Intelligence
Feasibility Studies
Deep Learning
Automated Triage Radiation Biodosimetry: Integrating Imaging Flow Cytometry with High-Throughput Robotics to Perform the Cytokinesis-Block Micronucleus Assay.
Radiation research
Feb 19, 2019
The cytokinesis-block micronucleus (CBMN) assay has become a fully-validated and standardized method for radiation biodosimetry. The assay is typically performed using microscopy, which is labor intensive, time consuming and impractical after a large...
Effect of inter-individual variability in human liver cytochrome P450 isozymes on cyclophosphamide-induced micronucleus formation.
Mutation research. Genetic toxicology and environmental mutagenesis
Dec 5, 2018
We investigated the relationship between metabolic activities of cytochrome P450 (CYP) isozymes present in microsomal fractions derived from the livers of 78 donors and micronucleus induction by cyclophosphamide (CPA). Consequently, a wide inter-indi...
Investigation of genotoxic effects of paraben in cultured human lymphocytes.
Drug and chemical toxicology
Dec 27, 2017
Paraben is a phenolic derivative of benzoic acid extensively used as preservatives in food, pharmaceutical, and cosmetic industries due to its antimicrobial characteristics. The objective of this study was to evaluate the in vitro genotoxic effects o...
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