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Polymerase Chain Reaction

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Prevalence and genotypes of Giardia lamblia from stray dogs and cats in Guangdong, China.

Veterinary parasitology, regional studies and reports
Giardia lamblia is a worldwide zoonotic intestinal parasite that infects humans and a wide range of mammals including dogs and cats, causing giardiasis with diarrhea. To investigate the infection and distribution of G. lamblia genotypes from stray do...

Experimental infection of Friesian bulls with Theileria orientalis (Ikeda) and effects on the haematocrit, live weight, rectal temperature and activity.

Veterinary parasitology, regional studies and reports
Since 2012, New Zealand has suffered from an epidemic of infectious bovine anaemia associated with T. orientalis (Ikeda), an obligate intracellular protozoan parasite of cattle. Despite widespread agreement that T. orientalis (Ikeda) infection has im...

Giardia duodenalis assemblages in cats from Virginia, USA.

Veterinary parasitology, regional studies and reports
Giardia duodenalis is considered a species complex that is divided into 8 genetically distinct but morphologically identical assemblages (A-H). Assemblages C-H are generally host adapted, while A and B infect both people and animals and are considere...

Automation of the standard DNA differential extraction on the Hamilton AutoLys STAR system: A proof-of-concept study.

Forensic science international. Genetics
For several decades, a common approach for processing sexual assault evidence has been to use the "standard" differential extraction to separate the evidence into a non-sperm-cell fraction and a sperm-cell fraction for further analysis. In this stand...

Developmental validation of PACE™: Automated artifact identification and contributor estimation for use with GlobalFiler™ and PowerPlex® fusion 6c generated data.

Forensic science international. Genetics
DNA mixture interpretation remains one of the major challenges in forensic DNA analysis. DNA mixture samples are inherently complex due to several factors including the variations in the quantity of DNA, the presence of non-allelic artifactual peaks ...

Artificial intelligence and avian influenza: Using machine learning to enhance active surveillance for avian influenza viruses.

Transboundary and emerging diseases
Influenza A viruses are one of the most significant viral groups globally with substantial impacts on human, domestic animal and wildlife health. Wild birds are the natural reservoirs for these viruses, and active surveillance within wild bird popula...

An interpretation algorithm for molecular diagnosis of bacterial vaginosis in a maternity hospital using machine learning: proof-of-concept study.

Diagnostic microbiology and infectious disease
Allplex Bacterial vaginosis assay (Seegene, South Korea) is a molecular test for bacterial vaginosis (BV). A machine learning algorithm was devised on 200 samples (BV = 23, non-BV = 177) converting 7 identified bacterial strains polymerase chain reac...

A machine learning algorithm to increase COVID-19 inpatient diagnostic capacity.

PloS one
Worldwide, testing capacity for SARS-CoV-2 is limited and bottlenecks in the scale up of polymerase chain reaction (PCR-based testing exist. Our aim was to develop and evaluate a machine learning algorithm to diagnose COVID-19 in the inpatient settin...

Age estimation using bloodstain miRNAs based on massive parallel sequencing and machine learning: A pilot study.

Forensic science international. Genetics
Age estimation is one of the most important components in the practice of forensic science, especially for body fluids or stains at crime scenes. Recent studies have focused on the application of DNA methylation for chronological age determination in...

Application of machine learning algorithm and modified high resolution DNA melting curve analysis for molecular subtyping of Salmonella isolates from various epidemiological backgrounds in northern Thailand.

World journal of microbiology & biotechnology
Food poisoning from consumption of food contaminated with non-typhoidal Salmonella spp. is a global problem. A modified high resolution DNA melting curve analysis (m-HRMa) was introduced to provide effective discrimination among closely related HRM c...