AIMC Topic: Recombinant Proteins
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Functional expression of porcine interferon-α using a combinational strategy in Pichia pastoris GS115.
Enzyme and microbial technology
Dec 10, 2018
Porcine interferon-α (pIFN-α) could be used as the vaccine adjuvant to enhance the antiviral ability of porcine in swine industry. In here, a combinational strategy integrating codon optimization, multiple gene insertion, strong AOX1 promoter, and ef...
Discovering de novo peptide substrates for enzymes using machine learning.
Nature communications
Dec 7, 2018
The discovery of peptide substrates for enzymes with exclusive, selective activities is a central goal in chemical biology. In this paper, we develop a hybrid computational and biochemical method to rapidly optimize peptides for specific, orthogonal ...
CbpM and CbpG of Streptococcus Pneumoniae Elicit a High Protection in Mice Challenged with a Serotype 19F Pneumococcus.
Iranian journal of allergy, asthma, and immunology
Dec 3, 2018
Among many pneumococcal antigens, choline-binding proteins (CPBs) display a high immunogenicity in animal models. This study aims to determine the immunogenicity of CbpM, CbpG and CbpL proteins of Streptococcus pneumoniae in a mice model. The genes w...
Development of a sandwich ELISA for determining plasma prolactin concentration in domestic birds.
Domestic animal endocrinology
Nov 24, 2018
The present study was conducted to establish a sandwich ELISA for the determination of prolactin (PRL) concentrations in the plasma of domestic fowls. The assay uses a recombinant goose PRL as the reference standard, expressed in a eukaryotic system,...
Aptamer facilitated purification of functional proteins.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Dec 16, 2017
DNA aptamers are attractive capture probes for affinity chromatography since, in contrast to antibodies, they can be chemically synthesized and, in contrast to tag-specific capture probes (such as Nickel-NTA or Glutathione), they can be used for puri...
Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning.
Biochemistry
Oct 26, 2017
As one of the most intrinsically reactive amino acids, cysteine carries a variety of important biochemical functions, including catalysis and redox regulation. Discovery and characterization of cysteines with heightened reactivity will help annotate ...
A novel aspartic protease from Rhizomucor miehei expressed in Pichia pastoris and its application on meat tenderization and preparation of turtle peptides.
Food chemistry
Oct 23, 2017
A novel aspartic protease gene (RmproA) was cloned from the thermophilic fungus Rhizomucor miehei CAU432 and expressed in Pichia pastoris. The RmproA was successfully expressed in P. pastoris as an active extracellular protease. High protease activit...
Model-enabled gene search (MEGS) allows fast and direct discovery of enzymatic and transport gene functions in the marine bacterium .
The Journal of biological chemistry
Apr 26, 2017
Whereas genomes can be rapidly sequenced, the functions of many genes are incompletely or erroneously annotated because of a lack of experimental evidence or prior functional knowledge in sequence databases. To address this weakness, we describe here...
Genomics
Recombinant Proteins
Models, Genetic
Escherichia coli
Aquaculture
Computer Simulation
Reproducibility of Results
Animals
Molecular Sequence Annotation
Pacific Ocean
Gene Deletion
Open Reading Frames
Genomic Library
Genetic Complementation Test
Expert Systems
Bacterial Proteins
Hawaii
Aquatic Organisms
Species Specificity
High-Throughput Nucleotide Sequencing
Aliivibrio fischeri
Decapodiformes
Optimization of an Anti-poly(ethylene glycol) (anti-PEG) Cell-Based Capture System To Quantify PEG and PEGylated Molecules.
Analytical chemistry
Nov 30, 2016
Sensitive determination of the pharmacokinetics of PEGylated molecules can accelerate the process of drug development. Here, we combined different anti-PEG Fab expressing 293T cells as capture cells (293T/3.3, 293T/6.3, and 293T/15-2b cells) with fou...
A robust robotic high-throughput antibody purification platform.
Journal of chromatography. A
May 24, 2016
Monoclonal antibodies (mAbs) have become the fastest growing segment in the drug market with annual sales of more than 40 billion US$ in 2013. The selection of lead candidate molecules involves the generation of large repertoires of antibodies from w...
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