AIMC Topic: Recombinant Proteins
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Development of a sandwich ELISA for determining plasma prolactin concentration in domestic birds.
Domestic animal endocrinology
Nov 24, 2018
The present study was conducted to establish a sandwich ELISA for the determination of prolactin (PRL) concentrations in the plasma of domestic fowls. The assay uses a recombinant goose PRL as the reference standard, expressed in a eukaryotic system,...
Aptamer facilitated purification of functional proteins.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Dec 16, 2017
DNA aptamers are attractive capture probes for affinity chromatography since, in contrast to antibodies, they can be chemically synthesized and, in contrast to tag-specific capture probes (such as Nickel-NTA or Glutathione), they can be used for puri...
Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning.
Biochemistry
Oct 26, 2017
As one of the most intrinsically reactive amino acids, cysteine carries a variety of important biochemical functions, including catalysis and redox regulation. Discovery and characterization of cysteines with heightened reactivity will help annotate ...
A novel aspartic protease from Rhizomucor miehei expressed in Pichia pastoris and its application on meat tenderization and preparation of turtle peptides.
Food chemistry
Oct 23, 2017
A novel aspartic protease gene (RmproA) was cloned from the thermophilic fungus Rhizomucor miehei CAU432 and expressed in Pichia pastoris. The RmproA was successfully expressed in P. pastoris as an active extracellular protease. High protease activit...
Model-enabled gene search (MEGS) allows fast and direct discovery of enzymatic and transport gene functions in the marine bacterium .
The Journal of biological chemistry
Apr 26, 2017
Whereas genomes can be rapidly sequenced, the functions of many genes are incompletely or erroneously annotated because of a lack of experimental evidence or prior functional knowledge in sequence databases. To address this weakness, we describe here...
Computer Simulation
Genetic Complementation Test
Pacific Ocean
Gene Deletion
Open Reading Frames
Aquatic Organisms
Genomic Library
High-Throughput Nucleotide Sequencing
Decapodiformes
Expert Systems
Models, Genetic
Hawaii
Escherichia coli
Species Specificity
Aquaculture
Aliivibrio fischeri
Bacterial Proteins
Molecular Sequence Annotation
Reproducibility of Results
Animals
Genomics
Recombinant Proteins
Optimization of an Anti-poly(ethylene glycol) (anti-PEG) Cell-Based Capture System To Quantify PEG and PEGylated Molecules.
Analytical chemistry
Nov 30, 2016
Sensitive determination of the pharmacokinetics of PEGylated molecules can accelerate the process of drug development. Here, we combined different anti-PEG Fab expressing 293T cells as capture cells (293T/3.3, 293T/6.3, and 293T/15-2b cells) with fou...
A robust robotic high-throughput antibody purification platform.
Journal of chromatography. A
May 24, 2016
Monoclonal antibodies (mAbs) have become the fastest growing segment in the drug market with annual sales of more than 40 billion US$ in 2013. The selection of lead candidate molecules involves the generation of large repertoires of antibodies from w...
Expression of rabies virus glycoprotein in the methylotrophic yeast Pichia pastoris.
Biotechnology and applied biochemistry
Apr 14, 2016
Rabies is a fatal disease that can be prevented by vaccination. Different approaches were investigated to develop novel human rabies vaccines with improved features compared to the current available vaccines, among them is the use of heterologous gen...
Mutagenic Potential ofBos taurus Papillomavirus Type 1 E6 Recombinant Protein: First Description.
BioMed research international
Dec 9, 2015
Bovine papillomavirus (BPV) is considered a useful model to study HPV oncogenic process. BPV interacts with the host chromatin, resulting in DNA damage, which is attributed to E5, E6, and E7 viral oncoproteins activity. However, the oncogenic mechani...
Prediction of recombinant protein overexpression in Escherichia coli using a machine learning based model (RPOLP).
Computers in biology and medicine
Sep 30, 2015
Recombinant protein overexpression, an important biotechnological process, is ruled by complex biological rules which are mostly unknown, is in need of an intelligent algorithm so as to avoid resource-intensive lab-based trial and error experiments i...
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