AIMC Topic: Staining and Labeling

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Training immunophenotyping deep learning models with the same-section ground truth cell label derivation method improves virtual staining accuracy.

Frontiers in immunology
INTRODUCTION: Deep learning (DL) models predicting biomarker expression in images of hematoxylin and eosin (H&E)-stained tissues can improve access to multi-marker immunophenotyping, crucial for therapeutic monitoring, biomarker discovery, and person...

Improvement of gram staining effect by ethanol pretreatment and quantization of staining image by unsupervised machine learning.

Archives of microbiology
In this study, we propose an Ethanol Pretreatment Gram staining method that significantly enhances the color contrast of the stain, thereby improving the accuracy of judgement, and demonstrated the effectiveness of the modification by eliminating una...

Unsupervised stain augmentation enhanced glomerular instance segmentation on pathology images.

International journal of computer assisted radiology and surgery
PURPOSE: In pathology images, different stains highlight different glomerular structures, so a supervised deep learning-based glomerular instance segmentation model trained on individual stains performs poorly on other stains. However, it is difficul...

Using machine learning for chemical-free histological tissue staining.

Journal of histotechnology
Hematoxylin and eosin staining can be hazardous, expensive, and prone to error and variability. To circumvent these issues, artificial intelligence/machine learning models such as generative adversarial networks (GANs), are being used to 'virtually' ...

RegWSI: Whole slide image registration using combined deep feature- and intensity-based methods: Winner of the ACROBAT 2023 challenge.

Computer methods and programs in biomedicine
BACKGROUND AND OBJECTIVE: The automatic registration of differently stained whole slide images (WSIs) is crucial for improving diagnosis and prognosis by fusing complementary information emerging from different visible structures. It is also useful t...

Combining Artificial Intelligence and Simplified Image Processing for the Automatic Detection of Mycobacterium tuberculosis in Acid-fast Stain : A Cross-institute Training and Validation Study.

The American journal of surgical pathology
Tuberculosis (TB) poses a significant health threat in Taiwan, necessitating efficient detection methods. Traditional screening for acid-fast positive bacilli in acid-fast stain is time-consuming and prone to human error due to staining artifacts. To...

Lung Cancer Diagnosis on Virtual Histologically Stained Tissue Using Weakly Supervised Learning.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Lung adenocarcinoma (LUAD) is the most common primary lung cancer and accounts for 40% of all lung cancer cases. The current gold standard for lung cancer analysis is based on the pathologists' interpretation of hematoxylin and eosin (H&E)-stained ti...

Registered multi-device/staining histology image dataset for domain-agnostic machine learning models.

Scientific data
Variations in color and texture of histopathology images are caused by differences in staining conditions and imaging devices between hospitals. These biases decrease the robustness of machine learning models exposed to out-of-domain data. To address...