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Zebrafish

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Video-tracking of zebrafish (Danio rerio) as a biological early warning system using two distinct artificial neural networks: Probabilistic neural network (PNN) and self-organizing map (SOM).

Aquatic toxicology (Amsterdam, Netherlands)
Biological early warning systems (BEWS) are becoming very important tools in ecotoxicological studies because they can detect changes in the behavior of organisms exposed to toxic substances. In this work, a video tracking system was fully developed ...

Acute caffeine administration affects zebrafish response to a robotic stimulus.

Behavioural brain research
Zebrafish has been recently proposed as a valid animal model to investigate the fundamental mechanisms regulating emotional behavior and evaluate the modulatory effects exerted by psychoactive compounds. In this study, we propose a novel methodologic...

Live predators, robots, and computer-animated images elicit differential avoidance responses in zebrafish.

Zebrafish
Emotional disturbances constitute a major health issue affecting a considerable portion of the population in western countries. In this context, animal models offer a relevant tool to address the underlying biological determinants and to screen novel...

High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.

Genetics
Microinjection is a technique used for transgenesis, mutagenesis, cell labeling, cryopreservation, and in vitro fertilization in multiple single and multicellular organisms. Microinjection requires specialized skills and involves rate-limiting and la...

Graphene oxide enhanced the endocrine disrupting effects of bisphenol A in adult male zebrafish: Integrated deep learning and metabolomics studies.

The Science of the total environment
In our previous studies, it was found that graphene oxide (GO) reduced the endocrine disruption of bisphenol A (BPA) in zebrafish embryo and larvae, but through different mechanisms. In this study, adult male zebrafish were selected to further unders...

Artificial intelligence-driven phenotyping of zebrafish psychoactive drug responses.

Progress in neuro-psychopharmacology & biological psychiatry
Zebrafish (Danio rerio) are rapidly emerging in biomedicine as promising tools for disease modelling and drug discovery. The use of zebrafish for neuroscience research is also growing rapidly, necessitating novel reliable and unbiased methods of neur...

Quantitative neuronal morphometry by supervised and unsupervised learning.

STAR protocols
We present a protocol to characterize the morphological properties of individual neurons reconstructed from microscopic imaging. We first describe a simple procedure to extract relevant morphological features from digital tracings of neural arbors. T...

Multiscale, multi-perspective imaging assisted robotic microinjection of 3D biological structures.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Microinjection is a widely used technique employed by biologists with applications in transgenesis, cryopreservation, mutagenesis, labeling/dye injection and in-vitro fertilization. However, microinjection is an extremely laborious manual procedure, ...

A magnetically controlled soft miniature robotic fish with a flexible skeleton inspired by zebrafish.

Bioinspiration & biomimetics
The untethered miniature swimming robot actuation and control is difficult as the robot size becomes smaller, due to limitations of feasible miniaturized on-board components. Nature provides much inspiration for developing miniature robot. Here, a ne...

XGSEA: CROSS-species gene set enrichment analysis via domain adaptation.

Briefings in bioinformatics
MOTIVATION: Gene set enrichment analysis (GSEA) has been widely used to identify gene sets with statistically significant difference between cases and controls against a large gene set. GSEA needs both phenotype labels and expression of genes. Howeve...