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Escherichia coli

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Rapid detection of microbiota cell type diversity using machine-learned classification of flow cytometry data.

Communications biology
The study of complex microbial communities typically entails high-throughput sequencing and downstream bioinformatics analyses. Here we expand and accelerate microbiota analysis by enabling cell type diversity quantification from multidimensional flo...

Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping.

Nature communications
Predicting effects of gene regulatory elements (GREs) is a longstanding challenge in biology. Machine learning may address this, but requires large datasets linking GREs to their quantitative function. However, experimental methods to generate such d...

Synthesis Success Calculator: Predicting the Rapid Synthesis of DNA Fragments with Machine Learning.

ACS synthetic biology
The synthesis and assembly of long DNA fragments has greatly accelerated synthetic biology and biotechnology research. However, long turnaround times or synthesis failures create unpredictable bottlenecks in the design-build-test-learn cycle. We deve...

Talaromydien a and talaroisocoumarin A, new metabolites from the marine-sourced fungus sp. ZZ1616.

Natural product research
New talaromydien A and talaroisocoumarin A (), together with nine known compounds (-), were isolated from a culture of the marine-derived sp. ZZ1616 in potato dextrose broth medium. Structures of the new compounds were elucidated based on their HRE...

Prediction of Protein-Protein Interactions with Local Weight-Sharing Mechanism in Deep Learning.

BioMed research international
Protein-protein interactions (PPIs) are important for almost all cellular processes, including metabolic cycles, DNA transcription and replication, and signaling cascades. The experimental methods for identifying PPIs are always time-consuming and ex...

DeLTA: Automated cell segmentation, tracking, and lineage reconstruction using deep learning.

PLoS computational biology
Microscopy image analysis is a major bottleneck in quantification of single-cell microscopy data, typically requiring human oversight and curation, which limit both accuracy and throughput. To address this, we developed a deep learning-based image an...

Machine Learning in Mass Spectrometry: A MALDI-TOF MS Approach to Phenotypic Antibacterial Screening.

Journal of medicinal chemistry
Machine learning techniques can be applied to MALDI-TOF mass spectral data of drug-treated cells to obtain classification models which assign the mechanism of action of drugs. Here, we present an example application of this concept to the screening o...

Design of (quinolin-4-ylthio)carboxylic acids as new Escherichia coli DNA gyrase B inhibitors: machine learning studies, molecular docking, synthesis and biological testing.

Computational biology and chemistry
Spread of multidrug-resistant Escherichia coli clinical isolates is a main problem in the treatment of infectious diseases. Therefore, the modern scientific approaches in decision this problem require not only a prevention strategy, but also the deve...

Genome-scale transcriptional dynamics and environmental biosensing.

Proceedings of the National Academy of Sciences of the United States of America
Genome-scale technologies have enabled mapping of the complex molecular networks that govern cellular behavior. An emerging theme in the analyses of these networks is that cells use many layers of regulatory feedback to constantly assess and precisel...

Covalent Functionalization of Graphene Sheets with Different Moieties and Their Effects on Biological Activities.

ACS biomaterials science & engineering
The ongoing spread of multi-drug-resistant bacteria over the past few decades necessitates collateral efforts to develop new classes of antibacterial agents with different mechanisms of action. The utilization of graphene nanosheets has recently gain...