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Extracellular Matrix

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Development of an Anisotropically Organized Brain dECM Hydrogel-Based 3D Neuronal Culture Platform for Recapitulating the Brain Microenvironment in Vivo.

ACS biomaterials science & engineering
To mimic the brain tissue microenvironment in vitro, the biological and structural properties of the utilized system must be similar to those of the native brain in the microenvironment in vivo. To promote the bioactive (biological) properties of mat...

Substrate stiffness affects neural network activity in an extracellular matrix proteins dependent manner.

Colloids and surfaces. B, Biointerfaces
Neuronal growth, differentiation, extension, branching and neural network activity are strongly influenced by the mechanical property of extracellular matrix (ECM). However, the mechanism by which substrate stiffness regulates a neural network activi...

Deep Learning for Drug Discovery and Cancer Research: Automated Analysis of Vascularization Images.

IEEE/ACM transactions on computational biology and bioinformatics
Likely drug candidates which are identified in traditional pre-clinical drug screens often fail in patient trials, increasing the societal burden of drug discovery. A major contributing factor to this phenomenon is the failure of traditional in vitro...

Soft Ring-Shaped Cellu-Robots with Simultaneous Locomotion in Batches.

Advanced materials (Deerfield Beach, Fla.)
Untethered mini-robots can move single cells or aggregates to build complex constructs in confined spaces and may enable various biomedical applications such as regenerative repair in medicine and biosensing in bioengineering. However, a significant ...

Super resolution microscopy and deep learning identify Zika virus reorganization of the endoplasmic reticulum.

Scientific reports
The endoplasmic reticulum (ER) is a complex subcellular organelle composed of diverse structures such as tubules, sheets and tubular matrices. Flaviviruses such as Zika virus (ZIKV) induce reorganization of ER membranes to facilitate viral replicatio...

Network Analysis of Integrin Adhesion Complexes.

Methods in molecular biology (Clifton, N.J.)
Cell-surface adhesion receptors mediate interactions with the extracellular matrix (ECM) to control many fundamental aspects of cell behavior, including cell migration, survival, and proliferation. Integrin adhesion receptors recruit structural and s...

Engineered Extracellular Matrices with Integrated Wireless Microactuators to Study Mechanobiology.

Advanced materials (Deerfield Beach, Fla.)
Mechanobiology explores how forces regulate cell behaviors and what molecular machinery are responsible for the sensing, transduction, and modulation of mechanical cues. To this end, probing of cells cultured on planar substrates has served as a prim...

Effect of Oxidized LDL on Platelet Shape, Spreading, and Migration Investigated with Deep Learning Platelet Morphometry.

Cells
Platelets are functionally versatile blood cells involved in thrombosis, hemostasis, atherosclerosis, and immune response. Platelet interaction with the immediate microenvironment in blood, vasculature, and tissues alters platelet morphology. The qua...

Predicting and understanding arterial elasticity from key microstructural features by bidirectional deep learning.

Acta biomaterialia
Microstructural features and mechanical properties are closely related in all soft biological tissues. Both yet exhibit considerable inter-individual differences and are affected by factors such as aging and disease and its progression. Histological ...