AIMC Topic: Milk
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Novel ELISA based on fluorescent quenching of DNA-stabilized silver nanoclusters for detecting E. coli O157:H7.
Food chemistry
Dec 23, 2018
Escherichia coli O157:H7 (E. coli O157:H7) is a potential threat to human health; thus, a rapid and sensitive method for detecting it is necessary. We designed a single-stranded DNA that contained an appended block and anchoring block. The appended b...
Case study: Evaluating quarter and composite milk sampling for detection of subclinical intramammary infections in dairy cattle.
Preventive veterinary medicine
Dec 23, 2018
Our objective was to evaluate a 200,000 cells/mL somatic cell count (SCC) cut-point on both the quarter and composite level to determine its effectiveness at identifying subclinical mastitis infections in one commercial dairy herd in Central New York...
Mutations associated with change of susceptibility to lincosamides and/or macrolides in field and laboratory-derived Mycoplasma californicum strains in Japan, and development of a rapid detection method for these mutations.
Veterinary microbiology
Dec 21, 2018
Five mutations involved in changing of susceptibility to lincosamides and/or macrolides were investigated in field isolates of Mycoplasma californicum in Japan, and reconfirmed in laboratory-derived mutants. In addition, a quick and easy detection me...
Comparison of physical and behavioral traits between dairy cows with low and high somatic cell count.
Preventive veterinary medicine
Dec 14, 2018
The objective of this study was to examine associations of locomotion score, hygiene, body condition score (BCS), lying behavior, and milk production with dairy cow somatic cell count (SCC; low or high). Cows from 14 commercial free-stall dairy herds...
Development of a real-time nucleic acid sequence-based amplification assay for the rapid detection of Salmonella spp. from food.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Dec 3, 2018
Salmonella spp. is one of the most common foodborne infectious pathogen. This study aimed to develop a real-time nucleic acid sequence-based amplification (NASBA) assay for detecting Salmonella in foods. Primers and a molecular beacon targeting the S...
Specific and sensitive ELISA for measurement of IgE-binding variations of milk allergen β-lactoglobulin in processed foods.
Analytica chimica acta
Nov 26, 2018
Immunochemical detection of food allergens is usually based on the use of polyclonal or monoclonal immunoglobulins G (IgG) antibodies. However, due to differences in epitopes recognition between IgG and IgE, an epitope modification during food proces...
Paper-based fluorescent immunoassay for highly sensitive and selective detection of norfloxacin in milk at picogram level.
Talanta
Nov 24, 2018
Norfloxacin (NOR) in milk may influence mammalian cell replication and bring about a decrease in the efficiency for treating infection in humans. However, current techniques for detecting NOR usually require expensive instruments and trained personne...
Robot utilisation of pasture-based dairy cows with varying levels of milking frequency.
Animal : an international journal of animal bioscience
Nov 21, 2018
Achieving a consistent level of robot utilisation throughout 24 h maximises automatic milking system (AMS) utilisation. However, levels of robot utilisation in the early morning hours are typically low, caused by the diurnal feeding behaviour of cows...
Dihydropteroate synthase based sensor for screening multi-sulfonamides residue and its comparison with broad-specific antibody based immunoassay by molecular modeling analysis.
Analytica chimica acta
Nov 8, 2018
A biosensor that could simultaneously detect multi-analyte as many as in a single test is more favored when facing lots of samples for screening purpose. In such a biosensor, the recognition element with broad specificity and highly affinity is a key...
Milk from cows fed a diet with a high forage:concentrate ratio improves inflammatory state, oxidative stress, and mitochondrial function in rats.
Journal of dairy science
Dec 28, 2017
Excessive energy intake may evoke complex biochemical processes characterized by inflammation, oxidative stress, and impairment of mitochondrial function that represent the main factors underlying noncommunicable diseases. Because cow milk is widely ...
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