AIMC Topic: Cell Culture Techniques
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Heparan Sulfate Induces Necroptosis in Murine Cardiomyocytes: A Medical- Approach Combining Experiments and Machine Learning.
Frontiers in immunology
Mar 20, 2018
Life-threatening cardiomyopathy is a severe, but common, complication associated with severe trauma or sepsis. Several signaling pathways involved in apoptosis and necroptosis are linked to trauma- or sepsis-associated cardiomyopathy. However, the un...
Carboxybetaine-modified succinylated chitosan-based beads encourage pancreatic β-cells (Min-6) to form islet-like spheroids under in vitro conditions.
Journal of materials science. Materials in medicine
Dec 30, 2017
In vitro, pancreatic β-cells tend to reduce their ability to aggregate into islets and lose insulin-producing ability, likely due to insufficient cell-cell and cell-matrix interactions that are essential for β-cell retention, viability and functional...
Insulin Secretion
Lasers
Microscopy, Electron, Scanning
Surface Properties
Animals
Mice
Chitosan
Insulin
Spectroscopy, Fourier Transform Infrared
Microscopy, Fluorescence
Scattering, Radiation
Carbon
Cadherins
Insulin-Secreting Cells
Spheroids, Cellular
Cell Survival
Molecular Dynamics Simulation
Cell Culture Techniques
Trans-Activators
Betaine
Homeodomain Proteins
Segregation of Tetragenococcus halophilus and Zygosaccharomyces rouxii using W/O/W double emulsion for use in mixed culture fermentation.
Food research international (Ottawa, Ont.)
Nov 21, 2017
Antagonism in mixed culture fermentation can result in undesirable metabolic activity and negatively affect the fermentation process. Water-oil-water (W/O/W) double emulsions (DE) could be utilized in fermentation for segregating multiple species and...
Analysis of live cell images: Methods, tools and opportunities.
Methods (San Diego, Calif.)
Feb 27, 2017
Advances in optical microscopy, biosensors and cell culturing technologies have transformed live cell imaging. Thanks to these advances live cell imaging plays an increasingly important role in basic biology research as well as at all stages of drug ...
Basic fibroblast growth factor is critical to reprogramming buffalo (Bubalus bubalis) primordial germ cells into embryonic germ stem cell-like cells.
Theriogenology
Dec 29, 2016
Primordial germ cells (PGCs) are destined to form gametes in vivo, and they can be reprogrammed into pluripotent embryonic germ (EG) cells in vitro. Buffalo PGC have been reported to be reprogrammed into EG-like cells, but the identities of the major...
A Novel Robot System Integrating Biological and Mechanical Intelligence Based on Dissociated Neural Network-Controlled Closed-Loop Environment.
PloS one
Nov 2, 2016
We propose the architecture of a novel robot system merging biological and artificial intelligence based on a neural controller connected to an external agent. We initially built a framework that connected the dissociated neural network to a mobile r...
Machine Learning Approach to Automated Quality Identification of Human Induced Pluripotent Stem Cell Colony Images.
Computational and mathematical methods in medicine
Jul 14, 2016
The focus of this research is on automated identification of the quality of human induced pluripotent stem cell (iPSC) colony images. iPS cell technology is a contemporary method by which the patient's cells are reprogrammed back to stem cells and ar...
Pattern Recognition, Automated
False Positive Reactions
Cell Culture Techniques
Induced Pluripotent Stem Cells
Bayes Theorem
Least-Squares Analysis
Quality Control
Regenerative Medicine
Humans
Machine Learning
Algorithms
Reproducibility of Results
Support Vector Machine
Fibroblasts
Models, Statistical
Developing an automated robotic factory for novel stem cell therapy production.
Regenerative medicine
May 11, 2016
Investigation of the mutagenic and genotoxic activities of LLL-3, a STAT3 inhibitor.
Drug and chemical toxicology
Apr 10, 2016
LLL-3, an anthracene derived compound, has been shown to be a promising therapeutic agent for the treatment of some kinds of cancer such as chronic myeloid leukemia and glioblastoma. However, no data regarding the toxic properties of this compound ha...
Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System.
Journal of visualized experiments : JoVE
May 14, 2015
Continued advancement in pluripotent stem cell culture is closing the gap between bench and bedside for using these cells in regenerative medicine, drug discovery and safety testing. In order to produce stem cell derived biopharmaceutics and cells fo...
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