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Cell Culture Techniques

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Segregation of Tetragenococcus halophilus and Zygosaccharomyces rouxii using W/O/W double emulsion for use in mixed culture fermentation.

Food research international (Ottawa, Ont.)
Antagonism in mixed culture fermentation can result in undesirable metabolic activity and negatively affect the fermentation process. Water-oil-water (W/O/W) double emulsions (DE) could be utilized in fermentation for segregating multiple species and...

Basic fibroblast growth factor is critical to reprogramming buffalo (Bubalus bubalis) primordial germ cells into embryonic germ stem cell-like cells.

Theriogenology
Primordial germ cells (PGCs) are destined to form gametes in vivo, and they can be reprogrammed into pluripotent embryonic germ (EG) cells in vitro. Buffalo PGC have been reported to be reprogrammed into EG-like cells, but the identities of the major...

Investigation of the mutagenic and genotoxic activities of LLL-3, a STAT3 inhibitor.

Drug and chemical toxicology
LLL-3, an anthracene derived compound, has been shown to be a promising therapeutic agent for the treatment of some kinds of cancer such as chronic myeloid leukemia and glioblastoma. However, no data regarding the toxic properties of this compound ha...

A Novel Robot System Integrating Biological and Mechanical Intelligence Based on Dissociated Neural Network-Controlled Closed-Loop Environment.

PloS one
We propose the architecture of a novel robot system merging biological and artificial intelligence based on a neural controller connected to an external agent. We initially built a framework that connected the dissociated neural network to a mobile r...

Machine Learning Approach to Automated Quality Identification of Human Induced Pluripotent Stem Cell Colony Images.

Computational and mathematical methods in medicine
The focus of this research is on automated identification of the quality of human induced pluripotent stem cell (iPSC) colony images. iPS cell technology is a contemporary method by which the patient's cells are reprogrammed back to stem cells and ar...

Analysis of live cell images: Methods, tools and opportunities.

Methods (San Diego, Calif.)
Advances in optical microscopy, biosensors and cell culturing technologies have transformed live cell imaging. Thanks to these advances live cell imaging plays an increasingly important role in basic biology research as well as at all stages of drug ...

Heparan Sulfate Induces Necroptosis in Murine Cardiomyocytes: A Medical- Approach Combining Experiments and Machine Learning.

Frontiers in immunology
Life-threatening cardiomyopathy is a severe, but common, complication associated with severe trauma or sepsis. Several signaling pathways involved in apoptosis and necroptosis are linked to trauma- or sepsis-associated cardiomyopathy. However, the un...

Deep Learning for Drug Discovery and Cancer Research: Automated Analysis of Vascularization Images.

IEEE/ACM transactions on computational biology and bioinformatics
Likely drug candidates which are identified in traditional pre-clinical drug screens often fail in patient trials, increasing the societal burden of drug discovery. A major contributing factor to this phenomenon is the failure of traditional in vitro...

Environmental properties of cells improve machine learning-based phenotype recognition accuracy.

Scientific reports
To answer major questions of cell biology, it is often essential to understand the complex phenotypic composition of cellular systems precisely. Modern automated microscopes produce vast amounts of images routinely, making manual analysis nearly impo...