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Microfluidic Analytical Techniques

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Thread based electrofluidic platform for direct metabolite analysis in complex samples.

Analytica chimica acta
The application of electrophoresis upon commercial threads is investigated for development of low-cost diagnostics assays, designed for the matrix separation and quantification of low abundance metabolites in complex samples - in this work riboflavin...

Prescribed 3-D Direct Writing of Suspended Micron/Sub-micron Scale Fiber Structures via a Robotic Dispensing System.

Journal of visualized experiments : JoVE
A 3-axis dispensing system is utilized to control the initiating and terminating fiber positions and trajectory via the dispensing software. The polymer fiber length and orientation is defined by the spatial positioning of the dispensing system 3-axi...

Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.

Nature methods
Induced pluripotent stem cells (iPSCs) are an essential tool for modeling how causal genetic variants impact cellular function in disease, as well as an emerging source of tissue for regenerative medicine. The preparation of somatic cells, their repr...

Bubble-free electrokinetic flow with propylene carbonate.

Electrophoresis
For electroosmotic pumping, a large direct-current (DC) electric field (10+ V/cm) is applied across a liquid, typically an aqueous electrolyte. At these high voltages, water undergoes electrolysis to form hydrogen and oxygen, generating bubbles that ...

Machine Learning Based Single-Frame Super-Resolution Processing for Lensless Blood Cell Counting.

Sensors (Basel, Switzerland)
A lensless blood cell counting system integrating microfluidic channel and a complementary metal oxide semiconductor (CMOS) image sensor is a promising technique to miniaturize the conventional optical lens based imaging system for point-of-care test...

Multiparameter mechanical and morphometric screening of cells.

Scientific reports
We introduce a label-free method to rapidly phenotype and classify cells purely based on physical properties. We extract 15 biophysical parameters from cells as they deform in a microfluidic stretching flow field via high-speed microscopy and apply m...

Label-free detection of aggregated platelets in blood by machine-learning-aided optofluidic time-stretch microscopy.

Lab on a chip
According to WHO, about 10 million new cases of thrombotic disorders are diagnosed worldwide every year. Thrombotic disorders, including atherothrombosis (the leading cause of death in the US and Europe), are induced by occlusion of blood vessels, du...

Engineering reaction-diffusion networks with properties of neural tissue.

Lab on a chip
We present an experimental system of networks of coupled non-linear chemical reactors, which we theoretically model within a reaction-diffusion framework. The networks consist of patterned arrays of diffusively coupled nanoliter-scale reactors contai...

Thread/paper- and paper-based microfluidic devices for glucose assays employing artificial neural networks.

Electrophoresis
This paper describes the fabrication of and data collection from two microfluidic devices: a microfluidic thread/paper based analytical device (μTPAD) and 3D microfluidic paper-based analytical device (μPAD). Flowing solutions of glucose oxidase (GOx...

Modular soft robotic microdevices for dexterous biomanipulation.

Lab on a chip
We present a methodology for building biologically inspired, soft microelectromechanical systems (MEMS) devices. Our strategy combines several advanced techniques including programmable colloidal self-assembly, light-harvesting with plasmonic nanotra...