AIMC Topic: Chromatography, Liquid
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Simultaneous quantification of fluoxetine and norfluoxetine in colostrum and mature human milk using a 2-dimensional liquid chromatography-tandem mass spectrometry system.
Journal of pharmaceutical and biomedical analysis
Dec 20, 2017
A two-dimensional liquid chromatography system coupled to triple quadrupole tandem mass spectrometer (2D LC-MS/MS) was employed for the determination of fluoxetine (FLU) and norfluoxetine (N-FLU) in colostrum and mature milk by direct sample injectio...
Bionalytical validation study for the determination of unbound ambrisentan in human plasma using rapid equilibrium dialysis followed by ultra performance liquid chromatography coupled to mass spectrometry.
Journal of pharmaceutical and biomedical analysis
Dec 16, 2017
Ambrisentan is a highly selective endothelin-1 type A receptor antagonist indicated for use in the treatment of pulmonary hypertension. In this study an assay was developed and validated for the quantification of total and unbound (free) concentratio...
Vitamin C evaluation in foods for infants and young children by a rapid and accurate analytical method.
Food chemistry
Nov 14, 2017
The validation of a rapid, precise (RSD < 4.6%), reliable and sensitive (LOD = 0.026 µg/mL) liquid chromatography method for vitamin C quantification in foods (infant formulae, n = 4; follow-on formulae, n = 3; processed cereal based-foods, n = 7; an...
Development and validation of an LC-MS/MS method for quantification of NC-8 in rat plasma and its application to pharmacokinetic studies.
Journal of food and drug analysis
Nov 11, 2017
ent-16-Oxobeyeran-19-N-methylureido (NC-8) is a recently synthesized derivative of isosteviol that showed anti-hepatitis B virus (HBV) activity by disturbing replication and gene expression of the HBV and by inhibiting the host toll-like receptor 2/n...
Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning.
Biochemistry
Oct 26, 2017
As one of the most intrinsically reactive amino acids, cysteine carries a variety of important biochemical functions, including catalysis and redox regulation. Discovery and characterization of cysteines with heightened reactivity will help annotate ...
Simultaneous LC-MS/MS determination of 40 legal and illegal psychoactive drugs in breast and bovine milk.
Food chemistry
Oct 10, 2017
This work presents a fast, sensitive and reliable multi-residue methodology based on fat and protein precipitation and liquid chromatography-tandem mass spectrometry for the determination of common legal and illegal psychoactive drugs, and major meta...
A new cyclic lipopeptide isolated from Bacillus amyloliquefaciens HAB-2 and safety evaluation.
Pesticide biochemistry and physiology
Aug 31, 2017
Bacillus is the most widely studied biocontrol agent and has been extensively used in the development of biopesticides and fungicides. In this study, a new cyclic lipopeptide was isolated from Bacillus amyloliquefaciens HAB-2 by column chromatography...
Investigating the antifungal activity and mechanism of a microbial pesticide Shenqinmycin against Phoma sp.
Pesticide biochemistry and physiology
Aug 30, 2017
Tea white scab (TWS) is a major disease affecting tea trees in mid-elevation regions and often occurs during rainy seasons with low temperatures. This disease is caused by the fungal pathogen Phoma sp. TWS can infect young stems, tender leaves, and t...
Energy Metabolism
Plant Diseases
Fungal Proteins
Microbial Sensitivity Tests
Down-Regulation
Gene Expression Regulation, Fungal
RNA, Messenger
Antifungal Agents
Culture Media
Genes, Fungal
Tandem Mass Spectrometry
Chromatography, Liquid
Up-Regulation
Phenazines
Nanotechnology
Camellia sinensis
Plant Structures
Homeostasis
Oxidation-Reduction
Mycelium
Ascomycota
Root cause investigation of deviations in protein chromatography based on mechanistic models and artificial neural networks.
Journal of chromatography. A
Aug 1, 2017
In protein chromatography, process variations, such as aging of column or process errors, can result in deviations of the product and impurity levels. Consequently, the process performance described by purity, yield, or production rate may decrease. ...
Detection of lithospermate B in rat plasma at the nanogram level by LC/MS in multi reaction monitoring mode.
Journal of food and drug analysis
Jul 8, 2017
Low bioavailability and high binding affinity to plasma proteins led to the difficulty for the quantitative detection of lithospermate B (LSB) in plasma. This study aimed to develop a protocol for detecting LSB in plasma. A method was employed to qua...
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