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Enzyme-Linked Immunosorbent Assay

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S100 proteins, cytokines, and chemokines as tear biomarkers in children with juvenile idiopathic arthritis-associated uveitis.

Ocular immunology and inflammation
PURPOSE: Biomarkers for juvenile idiopathic arthritis-associated uveitis (JIA-U) are needed. We aimed to measure inflammatory biomarkers in tears as a non-invasive method to identify biomarkers of uveitis activity.

A Comparative Study of the Diagnostic Potential of Plasma and Erythrocytic α-Synuclein in Parkinson's Disease.

Neuro-degenerative diseases
BACKGROUND: Parkinson's disease (PD) is a neurodegenerative disease characterized by intracellular α-synuclein (α-Syn) deposition. Alternation of the α-Syn expression level in plasma or erythrocytes may be used as a potential PD biomarker. However, n...

Peptide arrays incubated with three collections of human sera from patients infected with mosquito-borne viruses.

F1000Research
Global outbreaks caused by emerging or re-emerging arthropod-borne viruses (arboviruses) are becoming increasingly more common. These pathogens include the mosquito-borne viruses belonging to the and genera. These viruses often cause non-specific ...

Broad-specificity ELISA with a heterogeneous strategy for sensitive detection of microcystins and nodularin.

Toxicon : official journal of the International Society on Toxinology
A highly sensitive and broadly specific competitive indirect enzyme-linked immunosorbent assay (ciELISA) method was developed for the simultaneous detection of nine microcystins (MCs) and nodularin (NOD) using MC-LR-keyhole limpet hemocyanin (KLH) fo...

Dynamic changes in specific anti-L-asparaginase antibodies generation during acute lymphoblastic leukemia treatment.

Pharmacological reports : PR
BACKGROUND: L-asparaginase (L-asp) remains one of the key components of acute lymphoblastic leukemia therapy. Immune reactions to the drug are associated with its diminished activity. The aim of the study was to determine the level of IgM, IgG and Ig...

Placental CD4 T cells isolated from preeclamptic women cause preeclampsia-like symptoms in pregnant nude-athymic rats.

Pregnancy hypertension
Preeclampsia (PE), new onset hypertension during pregnancy, is associated with a proinflammatory profile compared to normal pregnancy (NP). We hypothesize that CD4 T cells from PE patient placentas cause PE symptoms during pregnancy compared to those...

Serum Concentrations of Interferon Gamma (IFN-γ) in Patients with Psoriasis: Correlation with Clinical Type and Severity of the Disease.

Medical archives (Sarajevo, Bosnia and Herzegovina)
INTRODUCTION: The etiopathogenesis of psoriasis is still unclear but there is evidence that many of cytokines released by keratinocytes and inflammatory leukocytes may contribute to the induction or persistence of the inflammatory process in psoriasi...

[Single-chain antibodies from alpaca for the detection of Fasciola hepatica antigens].

Revista peruana de medicina experimental y salud publica
OBJECTIVES.: To produce recombinant single-chain antibodies from alpaca that will bind to the excreted-secreted (ES) Fasciola hepatica antigen with high affinity and specificity, so as to develop new diagnostic technologies of human and animal fascio...

Machine Learning to Quantify Humoral Selection in Human Lupus Tubulointerstitial Inflammation.

Frontiers in immunology
In human lupus nephritis, tubulointerstitial inflammation (TII) is associated with expansion of B cells expressing anti-vimentin antibodies (AVAs). The mechanism by which AVAs are selected is unclear. Herein, we demonstrate that AVA somatic hypermut...

A novel method based on infrared spectroscopic inception-resnet networks for the detection of the major fish allergen parvalbumin.

Food chemistry
We have developed a novel approach that involves inception-resnet network (IRN) modeling based on infrared spectroscopy (IR) for rapid and specific detection of the fish allergen parvalbumin. SDS-PAGE and ELISA were used to validate the new method. T...