AIMC Topic: Enzyme-Linked Immunosorbent Assay
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A novel method based on infrared spectroscopic inception-resnet networks for the detection of the major fish allergen parvalbumin.
Food chemistry
Sep 6, 2020
We have developed a novel approach that involves inception-resnet network (IRN) modeling based on infrared spectroscopy (IR) for rapid and specific detection of the fish allergen parvalbumin. SDS-PAGE and ELISA were used to validate the new method. T...
Mice
Enzyme-Linked Immunosorbent Assay
Support Vector Machine
Food Analysis
Neural Networks, Computer
Reproducibility of Results
Animals
Fish Proteins
Electrophoresis, Polyacrylamide Gel
Spectrophotometry, Infrared
Fish Products
Parvalbumins
Mice, Inbred BALB C
Food Hypersensitivity
Fishes
Allergens
A Comparative Study of the Diagnostic Potential of Plasma and Erythrocytic α-Synuclein in Parkinson's Disease.
Neuro-degenerative diseases
Jun 2, 2020
BACKGROUND: Parkinson's disease (PD) is a neurodegenerative disease characterized by intracellular α-synuclein (α-Syn) deposition. Alternation of the α-Syn expression level in plasma or erythrocytes may be used as a potential PD biomarker. However, n...
S100 proteins, cytokines, and chemokines as tear biomarkers in children with juvenile idiopathic arthritis-associated uveitis.
Ocular immunology and inflammation
May 28, 2020
PURPOSE: Biomarkers for juvenile idiopathic arthritis-associated uveitis (JIA-U) are needed. We aimed to measure inflammatory biomarkers in tears as a non-invasive method to identify biomarkers of uveitis activity.
Broad-specificity ELISA with a heterogeneous strategy for sensitive detection of microcystins and nodularin.
Toxicon : official journal of the International Society on Toxinology
Dec 6, 2019
A highly sensitive and broadly specific competitive indirect enzyme-linked immunosorbent assay (ciELISA) method was developed for the simultaneous detection of nine microcystins (MCs) and nodularin (NOD) using MC-LR-keyhole limpet hemocyanin (KLH) fo...
Peptide arrays incubated with three collections of human sera from patients infected with mosquito-borne viruses.
F1000Research
Nov 7, 2019
Global outbreaks caused by emerging or re-emerging arthropod-borne viruses (arboviruses) are becoming increasingly more common. These pathogens include the mosquito-borne viruses belonging to the and genera. These viruses often cause non-specific ...
Novel ELISA based on fluorescent quenching of DNA-stabilized silver nanoclusters for detecting E. coli O157:H7.
Food chemistry
Dec 23, 2018
Escherichia coli O157:H7 (E. coli O157:H7) is a potential threat to human health; thus, a rapid and sensitive method for detecting it is necessary. We designed a single-stranded DNA that contained an appended block and anchoring block. The appended b...
Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-B Signaling Pathway.
Mediators of inflammation
Dec 17, 2018
Esophageal squamous cell carcinoma is the most common type of squamous cell carcinoma. Grape seed proanthocyanidin extract (GSPE) is considered to exhibit anticancer activity against several different types of cancer. We aimed to determine whether GS...
Assessment of ovulation synchronization protocols in goats and use of pregnancy specific protein B (PSPB) enzyme linked immunsorbent assay (ELISA) to determine kid number at birth.
Domestic animal endocrinology
Nov 30, 2018
The efficacy of several protocols for ovulation synchronization and timed artificial insemination (TAI) in goats was examined. In addition, the relationship between levels of pregnancy specific protein B (PSPB) during gestation assessed with a commer...
Dinoprost
Female
Equipment Reuse
Gonadotropin-Releasing Hormone
Ovulation
Estrus Synchronization
Drug Delivery Systems
Insemination, Artificial
Litter Size
Animals
Pregnancy-Specific beta 1-Glycoproteins
Gestational Age
Pregnancy
Progesterone
Delayed-Action Preparations
Pregnancy Outcome
Enzyme-Linked Immunosorbent Assay
Goats
Dynamic changes in specific anti-L-asparaginase antibodies generation during acute lymphoblastic leukemia treatment.
Pharmacological reports : PR
Nov 28, 2018
BACKGROUND: L-asparaginase (L-asp) remains one of the key components of acute lymphoblastic leukemia therapy. Immune reactions to the drug are associated with its diminished activity. The aim of the study was to determine the level of IgM, IgG and Ig...
Specific and sensitive ELISA for measurement of IgE-binding variations of milk allergen β-lactoglobulin in processed foods.
Analytica chimica acta
Nov 26, 2018
Immunochemical detection of food allergens is usually based on the use of polyclonal or monoclonal immunoglobulins G (IgG) antibodies. However, due to differences in epitopes recognition between IgG and IgE, an epitope modification during food proces...
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