AIMC Topic: Enzyme-Linked Immunosorbent Assay

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Salivary and serum levels of soluble E-cadherin in patients with gastrointestinal cancers: A comparative study.

Journal of cancer research and therapeutics
AIM: According to the literature, high levels of salivary soluble E-cadherin may be lined to advanced stage and poor prognosis in cancers. This research aimed at comparing salivary and serum levels of soluble E-cadherin in cases with esophageal, gast...

Deep learning-assisted ultra-accurate smartphone testing of paper-based colorimetric ELISA assays.

Analytica chimica acta
Smartphone has long been considered as one excellent platform for disease screening and diagnosis, especially when combined with microfluidic paper-based analytical devices (μPADs) that feature low cost, ease of use, and pump-free operations. In this...

Machine Learning to Quantify Humoral Selection in Human Lupus Tubulointerstitial Inflammation.

Frontiers in immunology
In human lupus nephritis, tubulointerstitial inflammation (TII) is associated with expansion of B cells expressing anti-vimentin antibodies (AVAs). The mechanism by which AVAs are selected is unclear. Herein, we demonstrate that AVA somatic hypermut...

A novel method based on infrared spectroscopic inception-resnet networks for the detection of the major fish allergen parvalbumin.

Food chemistry
We have developed a novel approach that involves inception-resnet network (IRN) modeling based on infrared spectroscopy (IR) for rapid and specific detection of the fish allergen parvalbumin. SDS-PAGE and ELISA were used to validate the new method. T...

A Comparative Study of the Diagnostic Potential of Plasma and Erythrocytic α-Synuclein in Parkinson's Disease.

Neuro-degenerative diseases
BACKGROUND: Parkinson's disease (PD) is a neurodegenerative disease characterized by intracellular α-synuclein (α-Syn) deposition. Alternation of the α-Syn expression level in plasma or erythrocytes may be used as a potential PD biomarker. However, n...

S100 proteins, cytokines, and chemokines as tear biomarkers in children with juvenile idiopathic arthritis-associated uveitis.

Ocular immunology and inflammation
PURPOSE: Biomarkers for juvenile idiopathic arthritis-associated uveitis (JIA-U) are needed. We aimed to measure inflammatory biomarkers in tears as a non-invasive method to identify biomarkers of uveitis activity.

Broad-specificity ELISA with a heterogeneous strategy for sensitive detection of microcystins and nodularin.

Toxicon : official journal of the International Society on Toxinology
A highly sensitive and broadly specific competitive indirect enzyme-linked immunosorbent assay (ciELISA) method was developed for the simultaneous detection of nine microcystins (MCs) and nodularin (NOD) using MC-LR-keyhole limpet hemocyanin (KLH) fo...

Peptide arrays incubated with three collections of human sera from patients infected with mosquito-borne viruses.

F1000Research
Global outbreaks caused by emerging or re-emerging arthropod-borne viruses (arboviruses) are becoming increasingly more common. These pathogens include the mosquito-borne viruses belonging to the and genera. These viruses often cause non-specific ...

Novel ELISA based on fluorescent quenching of DNA-stabilized silver nanoclusters for detecting E. coli O157:H7.

Food chemistry
Escherichia coli O157:H7 (E. coli O157:H7) is a potential threat to human health; thus, a rapid and sensitive method for detecting it is necessary. We designed a single-stranded DNA that contained an appended block and anchoring block. The appended b...

Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-B Signaling Pathway.

Mediators of inflammation
Esophageal squamous cell carcinoma is the most common type of squamous cell carcinoma. Grape seed proanthocyanidin extract (GSPE) is considered to exhibit anticancer activity against several different types of cancer. We aimed to determine whether GS...