AIMC Topic: Fluorescence
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Highly Sensitive Detection of Chemically Modified Thio-Organophosphates by an Enzymatic Biosensing Device: An Automated Robotic Approach.
Sensors (Basel, Switzerland)
Mar 2, 2020
Pesticides represent some of the most common man-made chemicals in the world. Despite their unquestionable utility in the agricultural field and in the prevention of pest infestation in public areas of cities, pesticides and their biotransformation p...
Modelling of bioprocess non-linear fluorescence data for at-line prediction of etanercept based on artificial neural networks optimized by response surface methodology.
Talanta
Dec 23, 2019
In the last years, regulatory agencies in biopharmaceutical industry have promoted the design and implementation of Process Analytical Technology (PAT), which aims to develop rapid and high-throughput strategies for real-time monitoring of bioprocess...
Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning.
PloS one
Dec 2, 2019
Due to the overlapping emission spectra of fluorophores, fluorescence microscopy images often have bleed-through problems, leading to a false positive detection. This problem is almost unavoidable when the samples are labeled with three or more fluor...
Exploiting open source 3D printer architecture for laboratory robotics to automate high-throughput time-lapse imaging for analytical microbiology.
PloS one
Nov 19, 2019
Growth in open-source hardware designs combined with the low-cost of high performance optoelectronic and robotics components has supported a resurgence of in-house custom lab equipment development. We describe a low cost (below $700), open-source, fu...
Machine Learning-Assisted Array-Based Biomolecular Sensing Using Surface-Functionalized Carbon Dots.
ACS sensors
Oct 11, 2019
Fluorescent array-based sensing is an emerging differential sensing platform for sensitive detection of analytes in a complex environment without involving a conventional "lock and key" type-specific interaction. These sensing techniques mainly rely ...
Intra- and Interspecies Variability of Single-Cell Innate Fluorescence Signature of Microbial Cell.
Applied and environmental microbiology
Aug 29, 2019
Here we analyzed the innate fluorescence signature of the single microbial cell, within both clonal and mixed populations of microorganisms. We found that even very similarly shaped cells differ noticeably in their autofluorescence features and that ...
Support Vector Machine Classification of Nonmelanoma Skin Lesions Based on Fluorescence Lifetime Imaging Microscopy.
Analytical chemistry
Jul 31, 2019
Early diagnosis of malignant skin lesions is critical for prompt treatment and a clinical prognosis of skin cancers. However, it is difficult to precisely evaluate the development stage of nonmelanoma skin cancers because they are derived from the sa...
Automated Stoichiometry Analysis of Single-Molecule Fluorescence Imaging Traces via Deep Learning.
Journal of the American Chemical Society
Apr 18, 2019
The stoichiometry of protein complexes is precisely regulated in cells and is fundamental to protein function. Singe-molecule fluorescence imaging based photobleaching event counting is a new approach for protein stoichiometry determination under phy...
Virtual histological staining of unlabelled tissue-autofluorescence images via deep learning.
Nature biomedical engineering
Mar 4, 2019
The histological analysis of tissue samples, widely used for disease diagnosis, involves lengthy and laborious tissue preparation. Here, we show that a convolutional neural network trained using a generative adversarial-network model can transform wi...
Novel ELISA based on fluorescent quenching of DNA-stabilized silver nanoclusters for detecting E. coli O157:H7.
Food chemistry
Dec 23, 2018
Escherichia coli O157:H7 (E. coli O157:H7) is a potential threat to human health; thus, a rapid and sensitive method for detecting it is necessary. We designed a single-stranded DNA that contained an appended block and anchoring block. The appended b...
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