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Keratins

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Evaluation of tumor budding with virtual panCK stains generated by novel multi-model CNN framework.

Computer methods and programs in biomedicine
As the global incidence of cancer continues to rise rapidly, the need for swift and precise diagnoses has become increasingly pressing. Pathologists commonly rely on H&E-panCK stain pairs for various aspects of cancer diagnosis, including the detecti...

Deep learning approach for accurate prostate cancer identification and stratification using combined immunostaining of cytokeratin, p63, and racemase.

Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society
BACKGROUND: Prostate cancer (PCa) is the most frequently diagnosed cancer in men worldwide, affecting around 1.4 million individuals. Current PCa diagnosis relies on histological analysis of prostate biopsy samples, an activity that is both time-cons...

Controlled surface morphology and hydrophilicity of polycaprolactone toward human retinal pigment epithelium cells.

Materials science & engineering. C, Materials for biological applications
Applying scaffolds as a bed to enhance cell proliferation and even differentiation is one of the treatment of retina diseases such as age-related macular degeneration (AMD) which deteriorating photoreceptors and finally happening blindness. In this s...

Automated annotations of epithelial cells and stroma in hematoxylin-eosin-stained whole-slide images using cytokeratin re-staining.

The journal of pathology. Clinical research
The diagnosis of solid tumors of epithelial origin (carcinomas) represents a major part of the workload in clinical histopathology. Carcinomas consist of malignant epithelial cells arranged in more or less cohesive clusters of variable size and shape...

Integrative measurement analysis via machine learning descriptor selection for investigating physical properties of biopolymers in hairs.

Scientific reports
Integrative measurement analysis of complex subjects, such as polymers is a major challenge to obtain comprehensive understanding of the properties. In this study, we describe analytical strategies to extract and selectively associate compositional i...

Deep learning-based virtual cytokeratin staining of gastric carcinomas to measure tumor-stroma ratio.

Scientific reports
The tumor-stroma ratio (TSR) determined by pathologists is subject to intra- and inter-observer variability. We aimed to develop a computational quantification method of TSR using deep learning-based virtual cytokeratin staining algorithms. Patients ...

Machine Learning-based Prediction Model for Treatment of Acromegaly With First-generation Somatostatin Receptor Ligands.

The Journal of clinical endocrinology and metabolism
CONTEXT: Artificial intelligence (AI), in particular machine learning (ML), may be used to deeply analyze biomarkers of response to first-generation somatostatin receptor ligands (fg-SRLs) in the treatment of acromegaly.

SHIFT: speedy histological-to-immunofluorescent translation of a tumor signature enabled by deep learning.

Scientific reports
Spatially-resolved molecular profiling by immunostaining tissue sections is a key feature in cancer diagnosis, subtyping, and treatment, where it complements routine histopathological evaluation by clarifying tumor phenotypes. In this work, we presen...

Assessment of individual tumor buds using keratin immunohistochemistry: moderate interobserver agreement suggests a role for machine learning.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Tumor budding is a promising and cost-effective biomarker with strong prognostic value in colorectal cancer. However, challenges related to interobserver variability persist. Such variability may be reduced by immunohistochemistry and computer-aided ...

Cytokeratin-Supervised Deep Learning for Automatic Recognition of Epithelial Cells in Breast Cancers Stained for ER, PR, and Ki-67.

IEEE transactions on medical imaging
Immunohistochemistry (IHC) of ER, PR, and Ki-67 are routinely used assays in breast cancer diagnostics. Determination of the proportion of stained cells (labeling index) should be restricted on malignant epithelial cells, carefully avoiding tumor inf...