AIMC Topic: Real-Time Polymerase Chain Reaction
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Machine Learning to Detect Alzheimer's Disease from Circulating Non-coding RNAs.
Genomics, proteomics & bioinformatics
Dec 4, 2019
Blood-borne small non-coding (sncRNAs) are among the prominent candidates for blood-based diagnostic tests. Often, high-throughput approaches are applied to discover biomarker signatures. These have to be validated in larger cohorts and evaluated by ...
Effect of potential probiotic Leuconostoc mesenteroides FB111 in prevention of cholesterol absorption by modulating NPC1L1/PPARĪ±/SREBP-2 pathways in epithelial Caco-2 cells.
International microbiology : the official journal of the Spanish Society for Microbiology
Dec 5, 2018
Mustard kimchi consumption reduces cholesterol levels in rats. To identify lactic acid bacteria (LAB) in kimchi which exert this effect, 20 LAB isolates were evaluated for cholesterol reduction in an in vitro screen. The FB111 strain showed the highe...
Rapid Rule Out of Culture-Negative Bloodstream Infections by Use of a Novel Approach to Universal Detection of Bacteria and Fungi.
The journal of applied laboratory medicine
Nov 20, 2018
BACKGROUND: Currently it can take up to 5 days to rule out bloodstream infection. With the low yield of blood cultures (approximately 10%), a significant number of patients are potentially exposed to inappropriate therapy that can lead to adverse eve...
A novel helper qPCR system for platinum detection via Pt-DNA coordination.
Analytica chimica acta
Nov 8, 2018
A novel real-time polymerase chain reaction (qPCR) platform for the simple and robust detection of platinum is described for the first time. Compared with conventional qPCR, a helper template, which is related to the active template for performing qP...
Placental CD4 T cells isolated from preeclamptic women cause preeclampsia-like symptoms in pregnant nude-athymic rats.
Pregnancy hypertension
Nov 2, 2018
Preeclampsia (PE), new onset hypertension during pregnancy, is associated with a proinflammatory profile compared to normal pregnancy (NP). We hypothesize that CD4 T cells from PE patient placentas cause PE symptoms during pregnancy compared to those...
Rats, Nude
Vascular Endothelial Growth Factor Receptor-1
Adult
Animals
Humans
Rats
Real-Time Polymerase Chain Reaction
CD4-Positive T-Lymphocytes
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Interleukin-17
Pre-Eclampsia
Pregnancy
Disease Models, Animal
Arterial Pressure
Female
Placenta
Placentation
Young Adult
Automation of the Differential Digestion Process of Sexual Assault Evidence.
Journal of forensic sciences
Jul 25, 2018
Sexual assault evidence samples require the use of a specific process known as a differential digestion to separate sperm from nonsperm cells prior to DNA extraction. An automated differential digestion process was developed using a selective degrada...
Evaluation of a peroxygen-based disinfectant for inactivation of porcine epidemic diarrhea virus at low temperatures on metal surfaces.
Veterinary microbiology
Dec 27, 2017
Porcine epidemic diarrhea virus (PEDV) spread rapidly across the United States in part due to contaminated livestock trailers. The objective of this study was to test a peroxygen-based disinfectant for the ability to inactivate PEDV on aluminum surfa...
Monitoring bottlenose dolphin leukocyte cytokine mRNA responsiveness by qPCR.
PloS one
Dec 22, 2017
Both veterinarians caring for dolphins in managed populations and researchers monitoring wild populations use blood-based diagnostics to monitor bottlenose dolphin (Tursiops truncatus) health. Quantitative PCR (qPCR) can be used to assess cytokine tr...
Unsupervised Network Analysis of the Plastic Supraoptic Nucleus Transcriptome Predicts Caprin2 Regulatory Interactions.
eNeuro
Dec 21, 2017
The supraoptic nucleus (SON) is a group of neurons in the hypothalamus responsible for the synthesis and secretion of the peptide hormones vasopressin and oxytocin. Following physiological cues, such as dehydration, salt-loading and lactation, the SO...
A European multicenter study on the analytical performance of the VERIS HBV assay.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Dec 19, 2017
BACKGROUND: Hepatitis B viral load monitoring is an essential part of managing patients with chronic Hepatits B infection. Beckman Coulter has developed the VERIS HBV Assay for use on the fully automated Beckman Coulter DxN VERIS Molecular Diagnostic...
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