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Tandem Mass Spectrometry

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Effect of Wuziyanzong pill on metabolism of dapoxetine in vivo and in vitro.

Journal of pharmaceutical and biomedical analysis
In vitro incubation of rat liver microsomes with 30 μL of 100 μmol·L dapoxetine and 30 μL of 10, 100, 250, 500, 1000, 2500, or 5000 μg·mL Wuziyanzong pill was performed at 37 °C for 60 min. Dapoxetine concentration was analyzed by high performance li...

UHPLC-MS/MS method for simultaneous determination of carbamazepine and its seven major metabolites in serum of epileptic patients.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Carbamazepine (CBZ) was considered as the drug of choice in the treatment for various forms of epilepsy, yet the non-negligible adverse effects of CBZ suspend as the major concern for rational and efficient clinical medication. This study developed a...

A 6 Second Analytical Method for Quantitation of Tacrolimus in Whole Blood by Use of Laser Diode Thermal Desorption Tandem Mass Spectrometry.

The journal of applied laboratory medicine
BACKGROUND: Therapeutic drug monitoring of immunosuppressive drugs is imperative for organ transplant recipients. High-performance LC-MS/MS is considered gold standard; however, immunoassays provide rapid turnaround time. New technology was developed...

Effect of inter-individual variability in human liver cytochrome P450 isozymes on cyclophosphamide-induced micronucleus formation.

Mutation research. Genetic toxicology and environmental mutagenesis
We investigated the relationship between metabolic activities of cytochrome P450 (CYP) isozymes present in microsomal fractions derived from the livers of 78 donors and micronucleus induction by cyclophosphamide (CPA). Consequently, a wide inter-indi...

Ultra-high-performance liquid chromatography-mass spectrometry method for neutrophil gelatinase-associated lipocalin as a predictive biomarker in acute kidney injury.

Talanta
Neutrophil gelatinase associated lipocalin (NGAL) is a protein that was found to be overexpressed in acute kidney injury (AKI). The rise in NGAL concentration, both in urine or plasma, appears earlier than for other classical renal function markers s...

Skin surface lipid differences in sensitive skin caused by psychological stress and distinguished by support vector machine.

Journal of cosmetic dermatology
BACKGROUND: Sensitive skin refers to skin that tends to be highly responsive to stimuli such as physical, chemical, and psychological. Psychological factors play an important role in the pathology of sensitive skin.

Isolation and identification of immunomodulatory selenium-containing peptides from selenium-enriched rice protein hydrolysates.

Food chemistry
The RAW264.7 cell model was employed to screen immunomodulatory selenium-containing peptides from selenium-enriched rice protein hydrolysates (SPHs). Moreover, the selenium-containing peptides of high-activity protein hydrolysates were purified by Se...

A highly sensitive ultra-high performance liquid chromatography/tandem mass spectrometry method with in-source fragmentation for rapid quantification of raspberry ketone.

Journal of food and drug analysis
Raspberry ketone (RK) is the characteristic aromatic compound in raspberry (Rubus idaeus L.) with wide applications as food additive and anti-obesity agent. However, quantification of RK has presented difficulties in MS detection and reliable LC-MS m...

A rapid LC-MS/MS method for simultaneous determination of quetiapine and duloxetine in rat plasma and its application to pharmacokinetic interaction study.

Journal of food and drug analysis
Combinations of new antidepressants like duloxetine and second-generation antipsychotics like quetiapine are used in clinical treatment of major depressive disorder, as well as in forensic toxicology scenarios. The drug-drug interaction (DDI) between...

Urinary Polyamine Biomarker Panels with Machine-Learning Differentiated Colorectal Cancers, Benign Disease, and Healthy Controls.

International journal of molecular sciences
Colorectal cancer (CRC) is one of the most daunting diseases due to its increasing worldwide prevalence, which requires imperative development of minimally or non-invasive screening tests. Urinary polyamines have been reported as potential markers to...