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In Vitro Techniques

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Enhancing analysis throughput, sensitivity and specificity in LC/ESI-MS/MS assay of plasma 25-hydroxyvitamin D by derivatization with triplex 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD) isotopologues.

Journal of pharmaceutical and biomedical analysis
The plasma/serum concentration of 25-hydroxyvitamin D [25(OH)D] is a diagnostic index for vitamin D deficiency/insufficiency, which is associated with a wide range of diseases, such as rickets, cancer and diabetes. We have reported that the derivatiz...

Regulation of demethylation and re-expression of RASSF1A gene in hepatocellular carcinoma cell lines treated with NCTD in vitro.

Journal of cancer research and therapeutics
BACKGROUND: Hepatocellular carcinoma, a lethal malignant neoplasm with poor prognosis, has dismal results of surgical resection and chemoradiotherapy. Norcantharidin (NCTD), the demethylated analog of cantharidin derived from a traditional Chinese me...

Lion's Mane, Hericium erinaceus and Tiger Milk, Lignosus rhinocerotis (Higher Basidiomycetes) Medicinal Mushrooms Stimulate Neurite Outgrowth in Dissociated Cells of Brain, Spinal Cord, and Retina: An In Vitro Study.

International journal of medicinal mushrooms
Neurodegenerative disease is defined as a deterioration of the nervous system in the intellectual and cognitive capabilities. Statistics show that more than 80-90 million individuals age 65 and above in 2050 may be affected by neurodegenerative condi...

Characterizing the Input-Output Function of the Olfactory-Limbic Pathway in the Guinea Pig.

Computational intelligence and neuroscience
Nowadays the neuroscientific community is taking more and more advantage of the continuous interaction between engineers and computational neuroscientists in order to develop neuroprostheses aimed at replacing damaged brain areas with artificial devi...

Enhanced needle localization in ultrasound using beam steering and learning-based segmentation.

Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society
Segmentation of needles in ultrasound images remains a challenging problem. In this paper, we introduce a machine learning-based method for needle segmentation in 2D beam-steered ultrasound images. We used a statistical boosting approach to train a p...