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CD8-Positive T-Lymphocytes

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A multicentre verification study of the QuantiFERON-TB Gold Plus assay.

Tuberculosis (Edinburgh, Scotland)
OBJECTIVES: The aim of this verification study was to compare the QuantiFERON-TB Gold Plus (QFT-Plus) to the QuantiFERON-TB Gold In Tube (QFT-GIT). The new QFT-Plus test contains an extra antigen tube which, according to the manufacturer additionally...

Histone deacetylase 2 is decreased in peripheral blood pro-inflammatory CD8+ T and NKT-like lymphocytes following lung transplant.

Respirology (Carlton, Vic.)
BACKGROUND AND OBJECTIVE: Immunosuppression therapy following lung transplantation fails to prevent chronic rejection in many patients, which is associated with lack of suppression of cytotoxic mediators and pro-inflammatory cytokines in peripheral b...

Identification of Immune Signatures of Novel Adjuvant Formulations Using Machine Learning.

Scientific reports
Adjuvants have long been critical components of vaccines, but the exact mechanisms of their action and precisely how they alter or enhance vaccine-induced immune responses are often unclear. In this study, we used broad immunoprofiling of antibody, c...

Single T Cell Sequencing Demonstrates the Functional Role of TCR Pairing in Cell Lineage and Antigen Specificity.

Frontiers in immunology
Although structural studies of individual T cell receptors (TCRs) have revealed important roles for both the α and β chain in directing MHC and antigen recognition, repertoire-level immunogenomic analyses have historically examined the β chain alone....

Reagent-Free and Rapid Assessment of T Cell Activation State Using Diffraction Phase Microscopy and Deep Learning.

Analytical chemistry
CD8 T cells constitute an essential compartment of the adaptive immune system. During immune responses, naı̈ve T cells become functional, as they are primed with their cognate determinants by the antigen presenting cells. Current methods of identifyi...

SIMON, an Automated Machine Learning System, Reveals Immune Signatures of Influenza Vaccine Responses.

Journal of immunology (Baltimore, Md. : 1950)
Machine learning holds considerable promise for understanding complex biological processes such as vaccine responses. Capturing interindividual variability is essential to increase the statistical power necessary for building more accurate predictive...

CD4+ versus CD8+ T-lymphocyte identification in an integrated microfluidic chip using light scattering and machine learning.

Lab on a chip
T lymphocytes are a group of cells representing the main effectors of human adaptive immunity. Characterization of the most representative T-lymphocyte subclasses, CD4+ and CD8+, is challenging, but has a significant impact on clinical decisions. Up ...