AIMC Topic: Genes, Reporter

Clear Filters Showing 1 to 8 of 8 articles

Machine learning-assisted decoding of temporal transcriptional dynamics via fluorescent timer.

Nature communications Jul 1, 2025
Investigating the temporal dynamics of gene expression is crucial for understanding gene regulation across various biological processes. Using the Fluorescent Timer protein, the Timer-of-cell-kinetics-and-activity system enables analysis of transcrip...
Mice Neural Networks, Computer Mice, Inbred C57BL CRISPR-Cas Systems Spleen Forkhead Transcription Factors Machine Learning Transcription, Genetic T-Lymphocytes, Regulatory Flow Cytometry Enhancer Elements, Genetic Animals Gene Expression Regulation Genes, Reporter Single-Cell Analysis
View on PubMed DOI

ReFeaFi: Genome-wide prediction of regulatory elements driving transcription initiation.

PLoS computational biology Sep 7, 2021
Regulatory elements control gene expression through transcription initiation (promoters) and by enhancing transcription at distant regions (enhancers). Accurate identification of regulatory elements is fundamental for annotating genomes and understan...
Humans Promoter Regions, Genetic Molecular Sequence Annotation Enhancer Elements, Genetic Genes, Reporter Models, Genetic Regulatory Sequences, Nucleic Acid Mutation Transcription Initiation, Genetic Gene Expression Regulation Genome, Human Deep Learning Genome-Wide Association Study Computational Biology
View on PubMed DOI

Ranking of non-coding pathogenic variants and putative essential regions of the human genome.

Nature communications Nov 20, 2019
A gene is considered essential if loss of function results in loss of viability, fitness or in disease. This concept is well established for coding genes; however, non-coding regions are thought less likely to be determinants of critical functions. H...
Mutation Supervised Machine Learning Genetic Variation Genome, Human Computer Simulation DNA Gene Expression Enhancer Elements, Genetic Genes, Reporter Chromatin Nucleic Acid Conformation Introns INDEL Mutation Humans
View on PubMed DOI

Whole-genome deep-learning analysis identifies contribution of noncoding mutations to autism risk.

Nature genetics May 27, 2019
We address the challenge of detecting the contribution of noncoding mutations to disease with a deep-learning-based framework that predicts the specific regulatory effects and the deleterious impact of genetic variants. Applying this framework to 1,7...
Gene Expression Regulation Genome, Human Transcription, Genetic RNA, Untranslated Genes, Reporter RNA Processing, Post-Transcriptional Computational Biology Gene Expression Mutation Humans Alleles Algorithms Genomics Phenotype Genetic Predisposition to Disease Autism Spectrum Disorder Genetic Association Studies Deep Learning
View on PubMed DOI

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.

Nature chemical biology Feb 26, 2018
We developed a new way to engineer complex proteins toward multidimensional specifications using a simple, yet scalable, directed evolution strategy. By robotically picking mammalian cells that were identified, under a microscope, as expressing prote...
Mice Robotics Flow Cytometry Microscopy, Fluorescence Neurons Zebrafish Hippocampus Optogenetics Female Luminescent Proteins Animals Male Cell Separation Fluorescence Humans HEK293 Cells Gene Library Brain Directed Molecular Evolution Caenorhabditis elegans Protein Engineering Genes, Reporter
View on PubMed DOI

McEnhancer: predicting gene expression via semi-supervised assignment of enhancers to target genes.

Genome biology Oct 26, 2017
Transcriptional enhancers regulate spatio-temporal gene expression. While genomic assays can identify putative enhancers en masse, assigning target genes is a complex challenge. We devised a machine learning approach, McEnhancer, which links target g...
Deoxyribonuclease I Animals Transcription Factors Sequence Analysis, DNA Embryonic Development Enhancer Elements, Genetic Machine Learning Promoter Regions, Genetic Genes, Reporter Drosophila melanogaster Gene Expression Regulation, Developmental Nucleotide Motifs Histone Code
View on PubMed DOI

Comparative evaluation of different cultivars of Flos Chrysanthemi by an anti-inflammatory-based NF-κB reporter gene assay coupled to UPLC-Q/TOF MS with PCA and ANN.

Journal of ethnopharmacology Aug 29, 2015
ETHNOPHARMACOLOGICAL RELEVANCE: Flos Chrysanthemi (FC), a commonly used traditional Chinese medicine, has five major cultivars ("Boju", "Chuju", "Gongju", "Hangbaiju" and "Huaiju") from different sources. However, the active constituents of these cul...
Genes, Reporter Principal Component Analysis Chrysanthemum NF-kappa B Humans Drug Evaluation, Preclinical Anti-Inflammatory Agents Neural Networks, Computer Chromatography, High Pressure Liquid Tandem Mass Spectrometry HEK293 Cells
View on PubMed DOI

Cloning Strategies for the Generation of Recombinant Capripoxvirus Through the Use of Screening and Selection Markers.

Methods in molecular biology (Clifton, N.J.) Jan 1, 2022
The ability to manipulate capripoxvirus through gene knockouts and gene insertions has become an increasingly valuable research tool in elucidating the function of individual genes of capripoxvirus, as well as in the development of capripoxvirus-base...
Capripoxvirus Vaccines, Synthetic Escherichia coli Genes, Reporter Cloning, Molecular
View on PubMed DOI
Popular Topics
Recent Journals