AIMC Topic: Mitochondria

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Optimizing intervertebral disc cell metabolic phenotyping with machine learning and artificial neural networks.

Scientific reports
Biological phenotyping of cellular metabolism is essential for deciphering health and disease states. The Seahorse XF analyzer enables direct measurement of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), providing insight ...

Mitochondria-Targeted Fluorescent Probes: Bridging Molecular Innovation and Disease Diagnosis.

Journal of medicinal chemistry
Mitochondria play essential roles in cellular energy metabolism and biological processes, with their dysfunction implicated in various diseases. Mitochondria-targeted fluorescent probes have thus been developed to sensitively detect metabolites and m...

LETM2 regulates mitochondrial function and autophagy in diabetic foot ulcers: implications for oxidative stress and therapeutic targeting.

Functional & integrative genomics
Background A major complication of diabetes, diabetic foot ulcers (DFU), has been linked to mitochondrial dysfunction and oxidative stress. Here, we performed analysis of RNA sequencing data from publicly available databases, including single-cell as...

Identification of ferroptosis- and mitochondrial metabolism-related biomarkers and the potential molecular mechanisms of poor ovarian response.

Journal of ovarian research
BACKGROUND: Ferroptosis and mitochondrial metabolism are closely associated with the pathological processes of various diseases. However, the role of ferroptosis-related genes (FRGs) and mitochondrial metabolism-related genes (MMRGs) in poor ovarian ...

Morphological alterations of peridroplet mitochondria in human liver biopsy.

Scientific reports
Mitochondrial dysfunction and the accumulation of lipid droplets (LD) contribute to the pathogenesis of liver diseases. Mitochondria bound to LD, termed peridroplet mitochondria (PDM), form a subpopulation with distinct functions compared to cytoplas...

Mitochondria-associated endoplasmic reticulum membrane (MAM)-associated calpains system in preeclampsia.

BMC pregnancy and childbirth
BACKGROUND: Preeclampsia (PE) is a multisystem disorder characterized primarily by hypertension and proteinuria after 20 weeks of gestation, significantly impacting maternal and fetal health. The etiology remains unclear, but it is primarily attribut...

Single-cell analysis of oxidative phosphorylation protein expression in pancreatic islets in type 2 diabetes.

The Journal of endocrinology
Mitochondrial dysfunction is a key feature of type 2 diabetes and is closely linked to ageing, a major risk factor for the disease. This study investigated islet cell composition and mitochondrial oxidative phosphorylation protein expression in pancr...

Label-free robotic mitochondrial biopsy.

Science advances
Robotic micromanipulation has advanced cellular probing, yet achieving precise, minimally invasive intracellular operations without fluorescent labeling remains challenging. Fluorescent techniques often cause photodamage and cytotoxicity and interfer...

Single-cell multi-omics uncovers CPS1 as a breast cancer immune evasion therapeutic target.

Scientific reports
Despite significant advances in early detection and therapeutic interventions, breast cancer persists as the most frequently diagnosed malignancy and the leading cause of cancer-related deaths among women globally. Although multiple prognostic signat...

Backtracking metabolic dynamics in single cells predicts bacterial replication in human macrophages.

Nature communications
Accurately tracking dynamic state transitions is crucial for modeling and predicting biological outcomes, as it captures heterogeneity of cellular responses. To build a model to predict bacterial infection in single cells, we have monitored in parall...