AIMC Topic: Nucleic Acid Amplification Techniques

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A colorimetric Loop-mediated isothermal amplification (LAMP) assay based on HRP-mimicking molecular beacon for the rapid detection of Vibrio parahaemolyticus.

Biosensors & bioelectronics
In the world wide, food poisoning accidents related to Vibrio spp. are on the rise, even numbers of food poisoning by other foodborne pathogens are decreasing. Therefore, the requirement of the rapid, sensitive and convenient detection method for V. ...

Mutations associated with change of susceptibility to lincosamides and/or macrolides in field and laboratory-derived Mycoplasma californicum strains in Japan, and development of a rapid detection method for these mutations.

Veterinary microbiology
Five mutations involved in changing of susceptibility to lincosamides and/or macrolides were investigated in field isolates of Mycoplasma californicum in Japan, and reconfirmed in laboratory-derived mutants. In addition, a quick and easy detection me...

GeNets: a unified web platform for network-based genomic analyses.

Nature methods
Functional genomics networks are widely used to identify unexpected pathway relationships in large genomic datasets. However, it is challenging to compare the signal-to-noise ratios of different networks and to identify the optimal network with which...

Self-primer and self-template recycle rolling circle amplification strategy for sensitive detection of uracil-DNA glycosylase activity.

Analytica chimica acta
Sensitive and accurate detection of uracil-DNA glycosylase (UDG) activity is available for evaluating and validating their function in uracil base-excision repair (UBER) pathway and clinical diagnosis. Here, a sensitive and accurate method for UDG ac...

Nano-biotinylated liposome-based immunoassay for the ultrasensitive detection of protein biomarker in urine.

Talanta
With the development of proteomics and the continuous discovery of biomarkers of trace proteins, it is important to accurately quantify low abundance protein, especially in urine for clinical diagnostics. In this paper, we reported a novel nano-bioti...

Sensitive fluorescent detection of DNA methyltransferase using nicking endonuclease-mediated multiple primers-like rolling circle amplification.

Biosensors & bioelectronics
Sensitive and reliable detection of DNA methyltransferase (MTase) is of great significance for both early tumor diagnosis and therapy. In this study, a simple, label-free and sensitive DNA MTase-sensing method was developed on the basis of a nicking ...

Sensitive detection of DNA methyltransferase using the dendritic rolling circle amplification-induced fluorescence.

Analytica chimica acta
The analysis of DNA methylation and MTase activities is very important in the early clinical diagnosis of cancer, on purposes of providing insights into the mechanism of gene repression and developing novel drugs of treating methylation-related disea...

The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Karlodinium veneficum.

Harmful algae
The aim of this study was to develop a loop-mediated isothermal amplification (LAMP) combined with a chromatographic lateral flow dipstick (LFD) assay to rapidly and specifically detect the Karlodinium veneficum ITS gene. Four groups of LAMP primers ...

Inexpensive method for the quantitative estimation of hepatitis C virus RNA in blood plasma for low-resource settings using ML-based image intensity analysis of RT-LAMP products.

Lab on a chip
Hepatitis C virus (HCV) infection is a severe public health problem affecting nearly 3% of the world population. Of those affected, approximately 80% develop chronic infections. Initiating treatment through HCV RNA testing remains challenging, especi...