AIMC Topic: Nucleic Acid Amplification Techniques

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Sensitive fluorescent detection of DNA methyltransferase using nicking endonuclease-mediated multiple primers-like rolling circle amplification.

Biosensors & bioelectronics Dec 30, 2016
Sensitive and reliable detection of DNA methyltransferase (MTase) is of great significance for both early tumor diagnosis and therapy. In this study, a simple, label-free and sensitive DNA MTase-sensing method was developed on the basis of a nicking ...
HEK293 Cells DNA (Cytosine-5-)-Methyltransferase 1 G-Quadruplexes DNA-Cytosine Methylases Humans Enzyme Assays DNA Primers DNA Biosensing Techniques Spectrometry, Fluorescence HeLa Cells Nucleic Acid Amplification Techniques MCF-7 Cells DNA (Cytosine-5-)-Methyltransferases Endonucleases
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Sensitive detection of DNA methyltransferase using the dendritic rolling circle amplification-induced fluorescence.

Analytica chimica acta Dec 27, 2016
The analysis of DNA methylation and MTase activities is very important in the early clinical diagnosis of cancer, on purposes of providing insights into the mechanism of gene repression and developing novel drugs of treating methylation-related disea...
Nucleic Acid Amplification Techniques DNA Modification Methylases DNA Probes Fluorescence DNA Methylation Nucleic Acid Conformation DNA, Catalytic
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The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Karlodinium veneficum.

Harmful algae Dec 21, 2016
The aim of this study was to develop a loop-mediated isothermal amplification (LAMP) combined with a chromatographic lateral flow dipstick (LFD) assay to rapidly and specifically detect the Karlodinium veneficum ITS gene. Four groups of LAMP primers ...
Chromatography Dinoflagellida China RNA, Algal Sensitivity and Specificity Nucleic Acid Amplification Techniques RNA, Protozoan DNA, Ribosomal Spacer Phylogeny
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Accurate Diagnosis of Colorectal Cancer Using a Combination of Lectin-Induced Recombinase Polymerase Amplification and CRISPR/Cas12a Assay on a Point-of-Care Testing Platform with Deep Learning Assistant.

Analytical chemistry Jul 1, 2025
Specific glycosylation patterns on exosome surfaces represent novel diagnostic biomarkers for cancer liquid biopsy. Lectins can induce exosome aggregation through multiple bindings with exosomal glycoproteins. In this work, we developed a one-pot lec...
CRISPR-Cas Systems Bacterial Proteins Endodeoxyribonucleases Colorectal Neoplasms Nucleic Acid Amplification Techniques Exosomes Animals Limit of Detection Lectins Cell Line, Tumor CRISPR-Associated Proteins Recombinases Humans Mice Deep Learning Point-of-Care Testing
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Active capture-directed bimetallic nanosubstrate for enhanced SERS detection of Staphylococcus aureus by combining strand exchange amplification and wavelength-selective machine learning.

Biosensors & bioelectronics Jun 15, 2025
Staphylococcus aureus (S. aureus) is the leading risk factor for food safety and human health. Herein, a novel wavelength-selective machine learning -driven adaptive strand exchange amplification (SEA)/SERS biosensor was developed for rapid detection...
Milk Silver Staphylococcal Infections Staphylococcus aureus Nucleic Acid Amplification Techniques Metal Nanoparticles Limit of Detection Gold Animals Biosensing Techniques Spectrum Analysis, Raman Humans Machine Learning
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Deep Learning-Enhanced Hand-Driven Microfluidic Chip for Multiplexed Nucleic Acid Detection Based on RPA/CRISPR.

Advanced science (Weinheim, Baden-Wurttemberg, Germany) Jun 1, 2025
The early detection of high-risk human papillomavirus (HR-HPV) is crucial for the assessment and improvement of prognosis in cervical cancer. However, existing PCR-based screening methods suffer from inadequate accessibility, which dampens the enthus...
Humans Female Papillomavirus Infections Early Detection of Cancer Nucleic Acid Amplification Techniques Uterine Cervical Neoplasms Deep Learning Human papillomavirus 16 Clustered Regularly Interspaced Short Palindromic Repeats Human papillomavirus 18 Lab-On-A-Chip Devices
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The Evolution of Nucleic Acid-Based Diagnosis Methods from the (pre-)CRISPR to CRISPR era and the Associated Machine/Deep Learning Approaches in Relevant RNA Design.

Methods in molecular biology (Clifton, N.J.) Jan 1, 2025
Nucleic acid tests (NATs) are considered as gold standard in molecular diagnosis. To meet the demand for onsite, point-of-care, specific and sensitive, trace and genotype detection of pathogens and pathogenic variants, various types of NATs have been...
Molecular Diagnostic Techniques Nucleic Acid Amplification Techniques RNA Humans CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Machine Learning Deep Learning
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