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Optogenetics

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A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.

Nature chemical biology
We developed a new way to engineer complex proteins toward multidimensional specifications using a simple, yet scalable, directed evolution strategy. By robotically picking mammalian cells that were identified, under a microscope, as expressing prote...

Dissecting the Catecholamines: How New Approaches Will Facilitate the Distinction between Noradrenergic and Dopaminergic Systems.

ACS chemical neuroscience
To overcome the limitations of fast scan cyclic voltammetry (FSCV) to discriminate between catecholamines, we discuss new approaches to monitor the dynamics of these neurochemicals with high spatial, genetic, and temporal specificity.

Analysis of the role of wind information for efficient chemical plume tracing based on optogenetic silkworm moth behavior.

Bioinspiration & biomimetics
Many animals use olfactory information to search for feeding areas and other individuals in real time and with high efficiency. We focus on the chemical plume tracing (CPT) ability of male silkworm moths and investigate an efficient CPT strategy for ...

A Machine Learning Approach to Characterize the Modulation of the Hippocampal Rhythms Via Optogenetic Stimulation of the Medial Septum.

International journal of neural systems
The medial septum (MS) is a potential target for modulating hippocampal activity. However, given the multiple cell types involved, the changes in hippocampal neural activity induced by MS stimulation have not yet been fully characterized. We combined...

A bioinspired optoelectronically engineered artificial neurorobotics device with sensorimotor functionalities.

Nature communications
Development of the next generation of bio- and nano-electronics is inseparably connected to the innovative concept of emulation and reproduction of biological sensorimotor systems and artificial neurobotics. Here, we report for the first time princip...

Neuromuscular actuation of biohybrid motile bots.

Proceedings of the National Academy of Sciences of the United States of America
The integration of muscle cells with soft robotics in recent years has led to the development of biohybrid machines capable of untethered locomotion. A major frontier that currently remains unexplored is neuronal actuation and control of such muscle-...

Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics.

Nature methods
We engineered light-gated channelrhodopsins (ChRs) whose current strength and light sensitivity enable minimally invasive neuronal circuit interrogation. Current ChR tools applied to the mammalian brain require intracranial surgery for transgene deli...

Distinct signals in medial and lateral VTA dopamine neurons modulate fear extinction at different times.

eLife
Dopamine (DA) neurons are to encode reward prediction error (RPE), in addition to other signals, such as salience. While RPE is known to support learning, the role of salience in learning remains less clear. To address this, we recorded and manipulat...

A simple Ca-imaging approach to neural network analyses in cultured neurons.

Journal of neuroscience methods
BACKGROUND: Ca-imaging is a powerful tool to measure neuronal dynamics and network activity. To monitor network-level changes in cultured neurons, neuronal activity is often evoked by electrical or optogenetic stimulation and assessed using multi-ele...