AIMC Topic: Chromatography, High Pressure Liquid

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Simultaneous determination of 7 thiols associated proteins in lymphoma patients'serum and cerebrospinal fluid by UHPLC-HRMS technique.

Scientific reports
Thiol compounds can serve as markers for the antioxidant and prognostic status of lymphoma, playing a crucial role in early tumor diagnosis. However, their high polarity and lack of chromophores pose challenges for multivariate analysis. This study a...

Plasma Lyso-PE 22:6 and Lyso-PE 20:4 are associated with development of mild to moderate depression revealed by metabolomics: a pilot study.

BMC psychiatry
BACKGROUND: Mild to moderate depression (MMD), as an early stage of depression, has a high incidence and may progress to severe depression, even leading to suicide. The lack of effective screening and treatment is due to the unknown metabolic changes...

A novel UHPLC-HRMS method for simultaneous determination of 20 amino metabolites and proteins in lymphoma patients' cells and serum.

Scientific reports
Highly sensitive and selective monitoring of amino metabolites such as glutamine, arginine, tryptophan and related proteins played significant roles in early diagnosis and warning of lymphoma. But those limited abundance and lacked chromophore group ...

Targeted metabolomics reveals bioactive inflammatory mediators from gut into blood circulation in children with NAFLD.

NPJ biofilms and microbiomes
Altered gut metabolites are important for the inflammatory progression in children with NAFLD. Fecal and plasma samples were collected from 145 subjects including 53 non-alcoholic fatty liver (NAFL), 39 nonalcoholic steatohepatitis (NASH) and 53 obes...

Establishing identifiable characteristic fingerprints of mulberry leaves: Integrating chemical composition and bioactivity through machine learning.

Journal of ethnopharmacology
ETHNOPHARMACOLOGICAL RELEVANCE: Mulberry leaves (Morus alba L.) are used in traditional Chinese medicine to clear the lungs and dispel wind-heat. Despite their common use, chemical reference substance rely solely on rutin, which may not reflect their...

Advancing freshness classification of freshly squeezed fruit juice via integrated multivariate analysis and machine learning approaches.

Food chemistry
In this study, multivariate analysis (MVA) and machine learning (ML), combined with UHPLC-HRMS, were used to evaluate the freshness of apples for juice production based on the analysis of freshly squeezed apple juice. A total of eight stages of apple...

Study on the quantitative analysis of Tilianin based on Raman spectroscopy combined with deep learning.

PloS one
Tilianin is a commonly used pharmaceutical ingredient with various biological activities such as antioxidant, anti-inflammatory, and anticancer, which is able to exert antitumor effects by inhibiting tumor cell proliferation, inducing apoptosis and i...

Substrate Specificity of the Organic Cation Transporters MATE1 and MATE2K and Functional Overlap with OCT1 and OCT2.

Journal of medicinal chemistry
The multidrug and toxin extrusion proteins MATE1 and MATE2K may determine the pharmacokinetics and drug-drug interactions of many drugs. However, their substrate spectrum and synergy with organic cation transporters OCT1 and OCT2 remain incompletely ...

A preliminary study on cause‑of‑death discrimination and the pathological stage identification in acute ischemia heart disease (AIHD) based on plasma lipidomic technique and machine learning algorithms.

International journal of legal medicine
The sudden death discrimination of acute ischemia heart disease (AIHD) and the determination of the AIHD pathological stage are the difficulties in forensic medicine. More potential biomarkers with high sensitivity and specificity still need to be id...