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Cell Hypoxia

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A mitochondria-targeting nitroreductase fluorescent probe with large Stokes shift and long-wavelength emission for imaging hypoxic status in tumor cells.

Analytica chimica acta
Development of a mitochondria-targeting fluorescent probe with large Stokes shift and long-wavelength emission was benefit for accurate detection of hypoxic status, which was known as a major factor of the tumor physiology and influence important pat...

[Y-27632 protects H9c2 cardiomyocytes against hypoxia/reoxygenation injury by inhibiting autophagy pathway].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Objective To investigate the protective effect of preconditioning with Rho-kinase inhibitor Y-27632 on H9c2 cardiomyocytes against hypoxia/reoxygenation (H/R) injury, and explore its underlying mechanism. Methods H9c2 cardiomyocytes were divided into...

Monitoring vascular normalization induced by antiangiogenic treatment with (18)F-fluoromisonidazole-PET.

Molecular oncology
BACKGROUND: Rationalization of antiangiogenics requires biomarkers. Vascular re-normalization is one widely accepted mechanism of action for this drug class. The interstitium of tumors with abnormal vasculature is hypoxic. We sought to track vascular...

Reduced blood EPAC1 protein levels as a marker of severe coronary artery disease: the role of hypoxic foam cell-transformed smooth muscle cells.

Journal of translational medicine
BACKGROUND: Vascular smooth muscle cells loaded with cholesterol (foam-VSMCs) play a crucial role in the progression of human atherosclerosis. Exchange Protein Directly Activated by cAMP 1 (EPAC1) is a critical protein in the regulation of vascular t...

Exploring machine learning for untargeted metabolomics using molecular fingerprints.

Computer methods and programs in biomedicine
BACKGROUND: Metabolomics, the study of substrates and products of cellular metabolism, offers valuable insights into an organism's state under specific conditions and has the potential to revolutionise preventive healthcare and pharmaceutical researc...

Transcriptional regulation of hypoxic cancer cell metabolism and artificial intelligence.

Trends in cancer
Gene expression regulation in hypoxic tumor microenvironments is mediated by O responsive transcription factors (OR-TFs), fine-tuning cancer cell metabolic demand for O according to its availability. Here, we discuss key OR-TFs and emerging artificia...