AIMC Topic: DNA, Bacterial

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Machine learning performance in a microbial molecular autopsy context: A cross-sectional postmortem human population study.

PloS one Apr 15, 2019
BACKGROUND: The postmortem microbiome can provide valuable information to a death investigation and to the human health of the once living. Microbiome sequencing produces, in general, large multi-dimensional datasets that can be difficult to analyze ...
Cross-Sectional Studies Male Middle Aged Adolescent Time Factors Aged Aged, 80 and over Young Adult Adult Autopsy High-Throughput Nucleotide Sequencing Postmortem Changes Datasets as Topic DNA, Archaeal DNA, Bacterial RNA, Ribosomal, 16S Sequence Analysis, DNA Humans Microbiota Machine Learning Female
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Rapid microarray-based assay for detection of pyrazinamide resistant Mycobacterium tuberculosis.

Diagnostic microbiology and infectious disease Dec 30, 2018
Pyrazinamide (PZA) is a key antibiotic for the treatment of drug susceptible tuberculosis. PZA-resistance is mainly mediated by mutations in the pncA gene; however the current gold standard is a phenotypic drug susceptibility test requiring a well-ad...
Mycobacterium tuberculosis Amidohydrolases Molecular Diagnostic Techniques Humans Eswatini DNA, Bacterial Pyrazinamide Sensitivity and Specificity Antitubercular Agents Microarray Analysis Time Factors Transition Temperature Tuberculosis, Multidrug-Resistant Genotype
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Antimicrobial Characteristics of Lactic Acid Bacteria Isolated from Homemade Fermented Foods.

BioMed research international Dec 30, 2018
. Lactic acid bacteria (LAB) were isolated from fermented foods, such as glutinous rice dough, corn noodle, chili sauce, potherb mustard pickles, and stinky tofu, in northeast China. LAB strains with antimicrobial activities were screened, and seven ...
Fermented Foods Lactobacillales RNA, Ribosomal, 16S Staphylococcus aureus Anti-Bacterial Agents Antimicrobial Cationic Peptides Microbial Sensitivity Tests Colony Count, Microbial DNA, Bacterial
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Novel ELISA based on fluorescent quenching of DNA-stabilized silver nanoclusters for detecting E. coli O157:H7.

Food chemistry Dec 23, 2018
Escherichia coli O157:H7 (E. coli O157:H7) is a potential threat to human health; thus, a rapid and sensitive method for detecting it is necessary. We designed a single-stranded DNA that contained an appended block and anchoring block. The appended b...
Sensitivity and Specificity Escherichia coli O157 DNA, Bacterial Animals Gold Enzyme-Linked Immunosorbent Assay Fluorescence Metal Nanoparticles Food Contamination Silver Food Microbiology Milk
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Mutations associated with change of susceptibility to lincosamides and/or macrolides in field and laboratory-derived Mycoplasma californicum strains in Japan, and development of a rapid detection method for these mutations.

Veterinary microbiology Dec 21, 2018
Five mutations involved in changing of susceptibility to lincosamides and/or macrolides were investigated in field isolates of Mycoplasma californicum in Japan, and reconfirmed in laboratory-derived mutants. In addition, a quick and easy detection me...
Japan Milk Mastitis, Bovine Lincosamides Macrolides Mycoplasma Cattle DNA, Bacterial Nucleic Acid Amplification Techniques Mutation Anti-Bacterial Agents Drug Resistance, Bacterial Female Animals
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Evaluation of a Urine-Based Rapid Molecular Diagnostic Test with Potential to Be Used at Point-of-Care for Pulmonary Tuberculosis: Cape Town Cohort.

The Journal of molecular diagnostics : JMD Dec 18, 2017
Tuberculosis (TB) diagnosis among sputum-scarce patients is time consuming. Thus, a nonsputum diagnostic alternative is urgently needed. The Mycobacterium tuberculosis-specific transrenal (Tr) DNA from urine is a potential target for TB diagnostics. ...
Mycobacterium tuberculosis Diagnostic Tests, Routine Polymerase Chain Reaction Multivariate Analysis Sputum Young Adult South Africa Adult Microscopy Male Sensitivity and Specificity Biomarkers Tuberculosis, Pulmonary Point-of-Care Systems Cross-Sectional Studies Female Molecular Diagnostic Techniques Humans Middle Aged Risk Factors DNA, Bacterial Aged HIV Infections Logistic Models Aged, 80 and over Cohort Studies Universities
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First study in Qatar to reveal high Legionella counts in cooling towers.

Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit Dec 14, 2017
Legionella spp. is transmitted from water to humans by aerosol-generating devices, including cooling towers (CTs). There have not been published reports about Legionella in these systems in Qatar. Ten CTs in Qatar University were sampled on a monthly...
Humans Air Conditioning DNA, Bacterial Legionella Water Microbiology Qatar
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Designing primers and evaluation of the efficiency of propidium monoazide - Quantitative polymerase chain reaction for counting the viable cells of Lactobacillus gasseri and Lactobacillus salivarius.

Journal of food and drug analysis Nov 29, 2016
The purpose of this study is to evaluate the efficiency of using propidium monoazide (PMA) real-time quantitative polymerase chain reaction (qPCR) to count the viable cells of Lactobacillus gasseri and Lactobacillus salivarius in probiotic products. ...
Lactobacillus gasseri Ligilactobacillus salivarius Bacterial Load Lactobacillus Polymerase Chain Reaction Azides Real-Time Polymerase Chain Reaction DNA Primers Microbial Viability Propidium DNA, Bacterial
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Comparison of Boiling and Robotics Automation Method in DNA Extraction for Metagenomic Sequencing of Human Oral Microbes.

PloS one Apr 22, 2016
The rapid improvement of next-generation sequencing performance now enables us to analyze huge sample sets with more than ten thousand specimens. However, DNA extraction can still be a limiting step in such metagenomic approaches. In this study, we a...
Humans Male Pilot Projects Principal Component Analysis Robotics Liquid Phase Microextraction Microbiota Dental Plaque DNA, Bacterial RNA, Ribosomal, 16S Sequence Analysis, DNA Adsorption High-Throughput Nucleotide Sequencing Metagenomics Hot Temperature
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Artificial Intelligence for the Evaluation of Operational Parameters Influencing Nitrification and Nitrifiers in an Activated Sludge Process.

Microbial ecology Feb 23, 2016
Nitrification at a full-scale activated sludge plant treating municipal wastewater was monitored over a period of 237 days. A combination of fluorescent in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (qPCR) were use...
In Situ Hybridization, Fluorescence Sewage Temperature Ammonia Artificial Intelligence Waste Management Real-Time Polymerase Chain Reaction Bacteria Nitrification DNA, Bacterial Nitrobacter Models, Theoretical
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