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Polymerase Chain Reaction

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Nanoparticle based bio-bar code technology for trace analysis of aflatoxin B1 in Chinese herbs.

Journal of food and drug analysis
A novel and sensitive assay for aflatoxin B1 (AFB1) detection has been developed by using bio-bar code assay (BCA). The method that relies on polyclonal antibodies encoded with DNA modified gold nanoparticle (NP) and monoclonal antibodies modified ma...

Evaluation of a human adenovirus viral load assay using the Altona RealStar® PCR test.

Diagnostic microbiology and infectious disease
This study evaluated the performance of the Altona Diagnostics RealStar® Adenovirus Research Use Only (RUO) real-time PCR reagents for HAdV quantitation in plasma samples from immunodeficient patients. The assay was linear from 2.30-9.17 log copies/m...

Genotyping of Giardia duodenalis in vegetables cultivated with organic and chemical fertilizer from street markets and community vegetable gardens in a region of Southern Brazil.

Transactions of the Royal Society of Tropical Medicine and Hygiene
BACKGROUND: In order to investigate the occurrence of Giardia duodenalis and its genotypes in vegetables that are consumed raw, we analyzed samples cultivated with organic or chemical fertilizer, sold in street markets and from community vegetable ga...

Prevalence of cytomegalovirus disease in kidney transplant patients in an intensive care unit.

Revista Brasileira de terapia intensiva
OBJECTIVES: To define the frequency of cytomegalovirus disease among kidney transplant patients in an intensive care unit in which this complication was suspected and to identify predisposing factors and their possible impact on clinical outcome.

Predictive factors of spontaneous CMV DNAemia clearance in kidney transplantation.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
UNLABELLED: Cytomegalovirus (CMV) infection occurs frequently after solid organ transplantation. Therapeutic strategies, in particular when to start a curative treatment, has not yet been defined. The purpose of this study was to assess predictive fa...

Sludge reflects intra-amniotic inflammation with or without microorganisms.

American journal of reproductive immunology (New York, N.Y. : 1989)
PROBLEM: To investigate whether amniotic fluid (AF) "sludge" in patients with preterm labor (PTL) with intact membranes is related to intra-amniotic infection or inflammation.

Designing primers and evaluation of the efficiency of propidium monoazide - Quantitative polymerase chain reaction for counting the viable cells of Lactobacillus gasseri and Lactobacillus salivarius.

Journal of food and drug analysis
The purpose of this study is to evaluate the efficiency of using propidium monoazide (PMA) real-time quantitative polymerase chain reaction (qPCR) to count the viable cells of Lactobacillus gasseri and Lactobacillus salivarius in probiotic products. ...