AIMC Topic: Polymerase Chain Reaction

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Evaluation of a Urine-Based Rapid Molecular Diagnostic Test with Potential to Be Used at Point-of-Care for Pulmonary Tuberculosis: Cape Town Cohort.

The Journal of molecular diagnostics : JMD Dec 18, 2017
Tuberculosis (TB) diagnosis among sputum-scarce patients is time consuming. Thus, a nonsputum diagnostic alternative is urgently needed. The Mycobacterium tuberculosis-specific transrenal (Tr) DNA from urine is a potential target for TB diagnostics. ...
Multivariate Analysis Tuberculosis, Pulmonary Mycobacterium tuberculosis Diagnostic Tests, Routine Polymerase Chain Reaction Universities Sputum South Africa Microscopy Aged, 80 and over HIV Infections Humans Young Adult Cohort Studies Molecular Diagnostic Techniques DNA, Bacterial Adult Logistic Models Biomarkers Sensitivity and Specificity Female Risk Factors Aged Cross-Sectional Studies Male Point-of-Care Systems Middle Aged
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Nanoparticle based bio-bar code technology for trace analysis of aflatoxin B1 in Chinese herbs.

Journal of food and drug analysis Dec 6, 2017
A novel and sensitive assay for aflatoxin B1 (AFB1) detection has been developed by using bio-bar code assay (BCA). The method that relies on polyclonal antibodies encoded with DNA modified gold nanoparticle (NP) and monoclonal antibodies modified ma...
Polymerase Chain Reaction Enzyme-Linked Immunosorbent Assay Drug Contamination Gold Aflatoxin B1 Metal Nanoparticles Electronic Data Processing Drugs, Chinese Herbal
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Evaluation of a human adenovirus viral load assay using the Altona RealStar® PCR test.

Diagnostic microbiology and infectious disease Dec 2, 2017
This study evaluated the performance of the Altona Diagnostics RealStar® Adenovirus Research Use Only (RUO) real-time PCR reagents for HAdV quantitation in plasma samples from immunodeficient patients. The assay was linear from 2.30-9.17 log copies/m...
Humans Prospective Studies Reproducibility of Results Viral Load Adenovirus Infections, Human Polymerase Chain Reaction Adenoviruses, Human Plasma
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Designing primers and evaluation of the efficiency of propidium monoazide - Quantitative polymerase chain reaction for counting the viable cells of Lactobacillus gasseri and Lactobacillus salivarius.

Journal of food and drug analysis Nov 29, 2016
The purpose of this study is to evaluate the efficiency of using propidium monoazide (PMA) real-time quantitative polymerase chain reaction (qPCR) to count the viable cells of Lactobacillus gasseri and Lactobacillus salivarius in probiotic products. ...
Real-Time Polymerase Chain Reaction DNA Primers Microbial Viability Bacterial Load Lactobacillus Polymerase Chain Reaction Propidium DNA, Bacterial Azides Lactobacillus gasseri Ligilactobacillus salivarius
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Malaria Parasitemia and Parasite Density in Antiretroviral-Treated HIV-Infected Adults Following Discontinuation of Cotrimoxazole Prophylaxis.

The Journal of infectious diseases Oct 25, 2016
BACKGROUND:  Cotrimoxazole (CTX) discontinuation increases malaria incidence in human immunodeficiency virus (HIV)-infected individuals. Rates, quantity, and timing of parasitemia rebound following CTX remain undefined.
HIV Infections Anti-HIV Agents Medication Adherence Parasitemia Plasmodium falciparum Trimethoprim, Sulfamethoxazole Drug Combination Female Polymerase Chain Reaction Adult Malaria Kenya Humans Male Antimalarials Parasite Load Prevalence
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tarSVM: Improving the accuracy of variant calls derived from microfluidic PCR-based targeted next generation sequencing using a support vector machine.

BMC bioinformatics Jun 10, 2016
BACKGROUND: Targeted sequencing of discrete gene sets is a cost effective strategy to screen subjects for monogenic forms of disease. One method to achieve this pairs microfluidic PCR with next generation sequencing. The PCR step of this pipeline cre...
Alleles Microfluidics High-Throughput Nucleotide Sequencing Data Accuracy Polymerase Chain Reaction Humans Software Support Vector Machine Sequence Analysis, DNA
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Efavirenz pharmacogenetics in a cohort of Italian patients.

International journal of antimicrobial agents Dec 18, 2015
The pharmacogenetics and pharmacokinetics of efavirenz (EFV) have been widely studied, although data in the Italian population are limited. Single nucleotide polymorphisms (SNPs) in the CYP2B6 gene have been associated with increased EFV plasma conce...
Pharmacogenetics Anti-HIV Agents Polymorphism, Single Nucleotide Cyclopropanes Female Adult Male Middle Aged Constitutive Androstane Receptor Alkynes Benzoxazines Italy HIV-1 Polymerase Chain Reaction Plasma Humans Retrospective Studies
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In vitro activities and detection performances of cefmetazole and flomoxef for extended-spectrum β-lactamase and plasmid-mediated AmpC β-lactamase-producing Enterobacteriaceae.

Diagnostic microbiology and infectious disease Dec 11, 2015
To investigate the in vitro activities of cephamycins (cefmetazole and flomoxef) for extended-spectrum β-lactamase (ESBL)- and plasmid-mediated AmpC β-lactamase (pAmpC)-producing Enterobacteriaceae, a total of 574 third-generation cephalosporin-resis...
Japan Enterobacteriaceae Cephalosporins Polymerase Chain Reaction beta-Lactam Resistance Enterobacteriaceae Infections Cefmetazole Microbial Sensitivity Tests Genotype Sequence Analysis, DNA beta-Lactamases Humans Anti-Bacterial Agents
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Automated Classification and Cluster Visualization of Genotypes Derived from High Resolution Melt Curves.

PloS one Nov 25, 2015
INTRODUCTION: High Resolution Melting (HRM) following PCR has been used to identify DNA genotypes. Fluorescent dyes bounded to double strand DNA lose their fluorescence with increasing temperature, yielding different signatures for different genotype...
Humans Machine Learning DNA Polymerase Chain Reaction Software Genotyping Techniques Transition Temperature Algorithms Automation, Laboratory Genotype
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Use of Artificial Intelligence and Machine Learning Algorithms with Gene Expression Profiling to Predict Recurrent Nonmuscle Invasive Urothelial Carcinoma of the Bladder.

The Journal of urology Oct 13, 2015
PURPOSE: Due to the high recurrence risk of nonmuscle invasive urothelial carcinoma it is crucial to distinguish patients at high risk from those with indolent disease. In this study we used a machine learning algorithm to identify the genes in patie...
Predictive Value of Tests Neoplasm Staging Biopsy Humans Aged Risk Assessment Prognosis Artificial Intelligence Carcinoma, Transitional Cell Female Sensitivity and Specificity Machine Learning Gene Expression Profiling Polymerase Chain Reaction Algorithms Male Urinary Bladder Neoplasms Neoplasm Invasiveness
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