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Single-Cell Analysis

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Causal machine learning for single-cell genomics.

Nature genetics
Advances in single-cell '-omics' allow unprecedented insights into the transcriptional profiles of individual cells and, when combined with large-scale perturbation screens, enable measuring of the effect of targeted perturbations on the whole transc...

scMalignantFinder distinguishes malignant cells in single-cell and spatial transcriptomics by leveraging cancer signatures.

Communications biology
Single-cell RNA sequencing (scRNA-seq) is a powerful tool for characterizing tumor heterogeneity, yet accurately identifying malignant cells remains challenging. Here, we propose scMalignantFinder, a machine learning tool specifically designed to dis...

SIMVI disentangles intrinsic and spatial-induced cellular states in spatial omics data.

Nature communications
Spatial omics technologies enable analysis of gene expression and interaction dynamics in relation to tissue structure and function. However, existing computational methods may not properly distinguish cellular intrinsic variability and intercellular...

Deep learning image analysis for continuous single-cell imaging of dynamic processes in Plasmodium falciparum-infected erythrocytes.

Communications biology
Continuous high-resolution imaging of the disease-mediating blood stages of the human malaria parasite Plasmodium falciparum faces challenges due to photosensitivity, small parasite size, and the anisotropy and large refractive index of host erythroc...

Reconstructing 3D chromosome structures from single-cell Hi-C data with SO(3)-equivariant graph neural networks.

NAR genomics and bioinformatics
The spatial conformation of chromosomes and genomes of single cells is relevant to cellular function and useful for elucidating the mechanism underlying gene expression and genome methylation. The chromosomal contacts (i.e. chromosomal regions in spa...

scVAEDer: integrating deep diffusion models and variational autoencoders for single-cell transcriptomics analysis.

Genome biology
Discovering a lower-dimensional embedding of single-cell data can improve downstream analysis. The embedding should encapsulate both the high-level features and low-level variations. While existing generative models attempt to learn such low-dimensio...

Light scattering imaging modal expansion cytometry for label-free single-cell analysis with deep learning.

Computer methods and programs in biomedicine
BACKGROUND AND OBJECTIVE: Single-cell imaging plays a key role in various fields, including drug development, disease diagnosis, and personalized medicine. To obtain multi-modal information from a single-cell image, especially for label-free cells, t...

Mouse-Geneformer: A deep learning model for mouse single-cell transcriptome and its cross-species utility.

PLoS genetics
Deep learning techniques are increasingly utilized to analyze large-scale single-cell RNA sequencing (scRNA-seq) data, offering valuable insights from complex transcriptome datasets. Geneformer, a pre-trained model using a Transformer Encoder archite...

Graph neural networks for single-cell omics data: a review of approaches and applications.

Briefings in bioinformatics
Rapid advancement of sequencing technologies now allows for the utilization of precise signals at single-cell resolution in various omics studies. However, the massive volume, ultra-high dimensionality, and high sparsity nature of single-cell data ha...

scMUSCL: multi-source transfer learning for clustering scRNA-seq data.

Bioinformatics (Oxford, England)
MOTIVATION: Single-cell RNA sequencing (scRNA-seq) analysis relies heavily on effective clustering to facilitate numerous downstream applications. Although several machine learning methods have been developed to enhance single-cell clustering, most a...