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Flow Cytometry

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Consistent quantitative gene product expression: #2. Antigen intensities on bone marrow cells are invariant between individuals.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
Five reference populations in bone marrow specimens were identified by flow cytometry using specific combinations of reagents in order define the variation of gene product expression intensities both within and between individuals. Mature lymphocytes...

An open-source solution for advanced imaging flow cytometry data analysis using machine learning.

Methods (San Diego, Calif.)
Imaging flow cytometry (IFC) enables the high throughput collection of morphological and spatial information from hundreds of thousands of single cells. This high content, information rich image data can in theory resolve important biological differe...

Consistent quantitative gene product expression: #1. Automated identification of regenerating bone marrow cell populations using support vector machines.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
Identification and quantification of maturing hematopoietic cell populations in flow cytometry data sets is a complex and sometimes irreproducible step in data analysis. Supervised machine learning algorithms present promise to automatically classify...

Radiation Dose Estimation by Automated Cytogenetic Biodosimetry.

Radiation protection dosimetry
The dose from ionizing radiation exposure can be interpolated from a calibration curve fit to the frequency of dicentric chromosomes (DCs) at multiple doses. As DC counts are manually determined, there is an acute need for accurate, fully automated b...

THE DECADE OF THE RABiT (2005-15).

Radiation protection dosimetry
The RABiT (Rapid Automated Biodosimetry Tool) is a dedicated Robotic platform for the automation of cytogenetics-based biodosimetry assays. The RABiT was developed to fulfill the critical requirement for triage following a mass radiological or nuclea...

Genotoxic mode of action predictions from a multiplexed flow cytometric assay and a machine learning approach.

Environmental and molecular mutagenesis
Several endpoints associated with cellular responses to DNA damage as well as overt cytotoxicity were multiplexed into a miniaturized, "add and read" type flow cytometric assay. Reagents included a detergent to liberate nuclei, RNase and propidium io...

Effect of Pseudomonas aeruginosa on sperm capacitation and protein phosphorylation of boar spermatozoa.

Theriogenology
Several studies have reported the detrimental effects that bacteriospermia causes on boar sperm quality, but little is known about its effects on IVC. Considering that, the present study sought to evaluate the effects of different concentrations of P...

Toxicity of titanium dioxide nanoparticles on Pseudomonas putida.

Water research
The increasing use of engineered nanoparticles (NPs) in industrial and household applications will very likely lead to the release of such materials into the environment. As wastewater treatment plants (WWTPs) are usually the last barrier before the ...

Label-free and dynamic evaluation of cell-surface epidermal growth factor receptor expression via an electrochemiluminescence cytosensor.

Talanta
A label-free electrochemiluminescence (ECL) cytosensor was developed for dynamically evaluating of epidermal growth factor receptor (EGFR) expression on MCF-7 cancer cells based on the specific recognition of epidermal growth factor (EGF) with its re...

Methods for discovery and characterization of cell subsets in high dimensional mass cytometry data.

Methods (San Diego, Calif.)
The flood of high-dimensional data resulting from mass cytometry experiments that measure more than 40 features of individual cells has stimulated creation of new single cell computational biology tools. These tools draw on advances in the field of m...