AIMC Topic: Fluorescent Dyes

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Homogeneous DNA-only keypad locks enable one-pot assay of multi-inputs.

Chemical communications (Cambridge, England)
Homogeneous DNA-only keypad locks were built with multi-stranded scalable junction substrates and a series of double-stranded eliminators to differentially process correctly- and wrongly-added DNA inputs, respectively. Unlike conventional strategies ...

Dual-emission CdTe/AgInS photoluminescence probe coupled to neural network data processing for the simultaneous determination of folic acid and iron (II).

Analytica chimica acta
This work focused on the combination of CdTe and AgInS quantum dots in a dual-emission nanoprobe for the simultaneous determination of folic acid and Fe(II) in pharmaceutical formulations. The surface chemistry of the used QDs was amended with suitab...

Top-down machine learning approach for high-throughput single-molecule analysis.

eLife
Single-molecule approaches provide enormous insight into the dynamics of biomolecules, but adequately sampling distributions of states and events often requires extensive sampling. Although emerging experimental techniques can generate such large dat...

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches.

International journal of molecular sciences
Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection ...

A mitochondria-targeting nitroreductase fluorescent probe with large Stokes shift and long-wavelength emission for imaging hypoxic status in tumor cells.

Analytica chimica acta
Development of a mitochondria-targeting fluorescent probe with large Stokes shift and long-wavelength emission was benefit for accurate detection of hypoxic status, which was known as a major factor of the tumor physiology and influence important pat...

Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning.

PloS one
Due to the overlapping emission spectra of fluorophores, fluorescence microscopy images often have bleed-through problems, leading to a false positive detection. This problem is almost unavoidable when the samples are labeled with three or more fluor...

Inexpensive robotic system for standard and fluorescent imaging of protein crystals.

Acta crystallographica. Section F, Structural biology communications
Protein-crystallization imaging and classification is a labor-intensive process typically performed either by humans or by instruments that currently cost well over $100 000. This cost puts the use of crystallization-trial imaging outside the reach o...

Automated Stoichiometry Analysis of Single-Molecule Fluorescence Imaging Traces via Deep Learning.

Journal of the American Chemical Society
The stoichiometry of protein complexes is precisely regulated in cells and is fundamental to protein function. Singe-molecule fluorescence imaging based photobleaching event counting is a new approach for protein stoichiometry determination under phy...

Near-infrared mito-specific fluorescent probe for ratiometric detection and imaging of alkaline phosphatase activity with high sensitivity.

Journal of materials chemistry. B
Fluorescent probe-based analytical methods for biological species have gained increasing attention for their powerful detection and imaging capabilities. However, it is still occasionally problematic for accurate analyses due to the influence of the ...