AIMC Topic: HEK293 Cells

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Preparation, characterization and toxicological investigation of copper loaded chitosan nanoparticles in human embryonic kidney HEK-293 cells.

Materials science & engineering. C, Materials for biological applications
Metallic nanoparticles often attribute severe adverse effects to the various organs or tissues at the molecular level despite of their applications in medical, laboratory and industrial sectors. The present study highlights the preparation of copper ...

Cenderitide: structural requirements for the creation of a novel dual particulate guanylyl cyclase receptor agonist with renal-enhancing in vivo and ex vivo actions.

European heart journal. Cardiovascular pharmacotherapy
AIMS: Cenderitide is a novel dual natriuretic peptide (NP) receptor chimeric peptide activator, which targets the particulate guanylyl cyclase B (pGC-B) receptor and pGC-A unlike native NPs. Cenderitide was engineered to retain the anti-fibrotic prop...

Comparative evaluation of different cultivars of Flos Chrysanthemi by an anti-inflammatory-based NF-κB reporter gene assay coupled to UPLC-Q/TOF MS with PCA and ANN.

Journal of ethnopharmacology
ETHNOPHARMACOLOGICAL RELEVANCE: Flos Chrysanthemi (FC), a commonly used traditional Chinese medicine, has five major cultivars ("Boju", "Chuju", "Gongju", "Hangbaiju" and "Huaiju") from different sources. However, the active constituents of these cul...

Discovery of novel GluN1/GluN3A NMDA receptor inhibitors using a deep learning-based method.

Acta pharmacologica Sinica
Ligand-based drug discovery methods typically utilize pharmacophore similarities among molecules to screen for potential active compounds. Among these, scaffold hopping is a widely used ligand-based lead identification strategy that facilitates clini...

ModiDeC: a multi-RNA modification classifier for direct nanopore sequencing.

Nucleic acids research
RNA modifications play a crucial role in various cellular functions. Here, we present ModiDeC, a deep-learning-based classifier able to identify and distinguish multiple RNA modifications (N6-methyladenosine, inosine, pseudouridine, 2'-O-methylguanos...

m6A-SPP: Identification of RNA N6-methyladenosine modification sites through multi-source biological features and a hybrid deep learning architecture.

International journal of biological macromolecules
The N6-methyladenosine(m6A) modification plays crucial regulatory roles in various biological processes including gene expression regulation, RNA stability, splicing, and translation. Accurate prediction of m6A modification sites is essential for und...

Boi-Ogi-To, a Traditional Japanese Kampo Medicine, Promotes Cellular Excretion of Chloride and Water by Activating Volume-Sensitive Outwardly Rectifying Anion Channels.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology
The Japanese Kampo medicine Boi-ogi-to (BOT) is known as an effective therapeutic agent for edema and nephrosis by promoting the excretion of excess body fluids. Despite its empirical effectiveness, scientific evidence supporting its effectiveness re...

Predicting adenine base editing efficiencies in different cellular contexts by deep learning.

Genome biology
BACKGROUND: Adenine base editors (ABEs) enable the conversion of A•T to G•C base pairs. Since the sequence of the target locus influences base editing efficiency, efforts have been made to develop computational models that can predict base editing ou...

Deep and accurate detection of m6A RNA modifications using miCLIP2 and m6Aboost machine learning.

Nucleic acids research
N6-methyladenosine (m6A) is the most abundant internal RNA modification in eukaryotic mRNAs and influences many aspects of RNA processing. miCLIP (m6A individual-nucleotide resolution UV crosslinking and immunoprecipitation) is an antibody-based appr...

A machine learning-based framework for modeling transcription elongation.

Proceedings of the National Academy of Sciences of the United States of America
RNA polymerase II (Pol II) generally pauses at certain positions along gene bodies, thereby interrupting the transcription elongation process, which is often coupled with various important biological functions, such as precursor mRNA splicing and gen...