AIMC Topic: Single-Cell Analysis

Clear Filters Showing 41 to 50 of 679 articles

Single-cell RNA-seq combined with bulk RNA-seq analysis identifies necroptosis-related genes as therapeutic targets for periodontitis.

BMC medical genomics
BACKGROUND: Necroptosis, a regulated form of programmed cell death, exacerbates inflammatory responses by releasing damage-associated molecular patterns and inflammatory factors. However, the specific mechanisms underlying necroptosis in periodontiti...

A comprehensive benchmark of single-cell Hi-C embedding tools.

Nature communications
Embedding is the key step in single-cell Hi-C (scHi-C) analysis which relies on capturing biological meaningful heterogeneity at various levels of genome architecture. To understand the strength and limitations of existing tools in various applicatio...

Identification of lipid metabolism-related signature in nonalcoholic fatty liver: evidences from transcriptomics and single cell RNA-sequencing analysis.

European journal of medical research
BACKGROUND: Considering the complex and close-knit relationship between non-alcoholic fatty liver disease (NAFLD) and the metabolic status, this study aimed to identify lipid metabolism-related genes (LMGs), construct an effective diagnostic model, a...

Single-cell RNA sequencing and Mendelian randomization, revealing molecular mechanisms and causal correlation of immune-related genes in periodontitis.

Scientific reports
The immune system has been linked to periodontitis risk in oral inflammation and systemic consequences. Specifically, this study investigated whether hub genes were associated with immune cells via integrating single-cell RNA sequencing (scRNA-seq) a...

Pan-cancer single-cell and spatial transcriptomics implicate cancer-associated fibroblasts in neutrophil immunosuppressive phenotypic transitions and immunotherapy resistance.

Functional & integrative genomics
Neutrophils are the most abundant granulocyte population and have important functions such as defense against pathogens. However, they show significant Heterogeneity and play more complex roles in tumors. The theory of two-tiered differentiation of n...

Single-cell and spatial transcriptomics reveal post-translational modifications in osteosarcoma progression and tumor microenvironment.

PloS one
Emerging evidence suggests that post-translational modifications (PTMs) contribute to osteosarcoma pathogenesis, yet their exact molecular roles require further elucidation. Using the AddModuleScore method, we classified tumor cells on the basis of P...

Paired snRNA-seq and scRNA-seq analysis of MASLD patients to identify early-stage markers for disease progression.

Hepatology communications
BACKGROUND AND AIMS: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a leading cause of chronic liver disease worldwide. Progression from simple metabolic dysfunction-associated steatotic liver (MASL) without necro-inflammation to...

HCS-3DX, a next-generation AI-driven automated 3D-oid high-content screening system.

Nature communications
Self-organised three-dimensional (3D) cell cultures, collectively called 3D-oids, include spheroids, organoids and other co-culture models. Systematic evaluation of these models forms a critical new generation of high-content screening (HCS) systems ...

Characterization of SPTLC2 as a key driver promoting microglial activation and energy metabolism reprogramming after ischemic stroke through bulk and single-cell analyses combined with experimental validation.

Cell biology and toxicology
BACKGROUND: Ischemic stroke (IS) stands as a principal contributor to high rates of sickness and death. The condition's pathological development is complicated, featuring mechanisms like mitochondrial impairment and the activation of microglial cells...

Single-cell RNA-sequencing of circulating tumour cells: A practical guide to workflow and translational applications.

Cancer metastasis reviews
The global burden of cancer is rising, with treatment failures often due to the metastatic nature of late-stage malignancies. Circulating tumour cells (CTCs) are metastatic precursors shed from primary tumours, which survive in circulation, extravasa...