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Single-Cell Analysis

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Multi-omics and single-cell analysis reveals machine learning-based pyrimidine metabolism-related signature in the prognosis of patients with lung adenocarcinoma.

International journal of medical sciences
Pyrimidine metabolism is a hallmark of tumor metabolic reprogramming, while its significance in the prognostic and therapeutic implications of patients with lung adenocarcinoma (LUAD) still remains unclear. In this study, an integrated framework of...

Development of a tertiary lymphoid structure-based prognostic model for breast cancer: integrating single-cell sequencing and machine learning to enhance patient outcomes.

Frontiers in immunology
BACKGROUND: Breast cancer, a highly prevalent global cancer, poses significant challenges, especially in advanced stages. Prognostic models are crucial to enhance patient outcomes. Tertiary lymphoid structures (TLS) within the tumor microenvironment ...

stAI: a deep learning-based model for missing gene imputation and cell-type annotation of spatial transcriptomics.

Nucleic acids research
Spatial transcriptomics technology has revolutionized our understanding of cellular systems by capturing RNA transcript levels in their original spatial context. Single-cell spatial transcriptomics (scST) offers single-cell resolution expression leve...

Deep learning-based cell-specific gene regulatory networks inferred from single-cell multiome data.

Nucleic acids research
Gene regulatory networks (GRNs) provide a global representation of how genetic/genomic information is transferred in living systems and are a key component in understanding genome regulation. Single-cell multiome data provide unprecedented opportunit...

Identification of potential biomarkers in cardiovascular calcification based on bioinformatics combined with single-cell RNA-seq and multiple machine learning analysis.

Cellular signalling
BACKGROUND: The molecular and genetic mechanisms underlying vascular calcification remain unclear. This study aimed to determine the differences in calcification marker-related gene expression in macrophages.

Enhancing single-cell classification accuracy using image conversion and deep learning.

Yi chuan = Hereditas
Single-cell transcriptome sequencing (scRNA-seq) is widely used in the fields of animal and plant developmental biology and important trait analysis by obtaining single-cell transcript abundance data in high throughput, which can deeply reveal cell t...

scMDCL: A Deep Collaborative Contrastive Learning Framework for Matched Single-Cell Multiomics Data Clustering.

Journal of chemical information and modeling
Single-cell multiomics clustering integrates multiple omics data to analyze cellular heterogeneity and is crucial for uncovering complex biological processes and disease mechanisms. However, existing matched single-cell multiomics clustering methods ...

scHeteroNet: A Heterophily-Aware Graph Neural Network for Accurate Cell Type Annotation and Novel Cell Detection.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Single-cell RNA sequencing (scRNA-seq) has unveiled extensive cellular heterogeneity, yet precise cell type annotation and the identification of novel cell populations remain significant challenges. scHeteroNet, a novel graph neural network framework...

Investigation of cell development and tissue structure network based on natural Language processing of scRNA-seq data.

Journal of translational medicine
BACKGROUND: Single-cell multi-omics technologies, particularly single-cell RNA sequencing (scRNA-seq), have revolutionized our understanding of cellular heterogeneity and development by providing insights into gene expression at the single-cell level...

The role of senescence-related genes in major depressive disorder: insights from machine learning and single cell analysis.

BMC psychiatry
BACKGROUND: Evidence indicates that patients with Major Depressive Disorder (MDD) exhibit a senescence phenotype or an increased susceptibility to premature senescence. However, the relationship between senescence-related genes (SRGs) and MDD remains...