AIMC Topic: Cell Separation

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Oral epithelial cell segmentation from fluorescent multichannel cytology images using deep learning.

Computer methods and programs in biomedicine
BACKGROUND AND OBJECTIVES: Cytology is a proven, minimally-invasive cancer screening and surveillance strategy. Given the high incidence of oral cancer globally, there is a need to develop a point-of-care, automated, cytology-based screening tool. Or...

Intelligent sort-timing prediction for image-activated cell sorting.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
Intelligent image-activated cell sorting (iIACS) has enabled high-throughput image-based sorting of single cells with artificial intelligence (AI) algorithms. This AI-on-a-chip technology combines fluorescence microscopy, AI-based image processing, s...

Fast and precise single-cell data analysis using a hierarchical autoencoder.

Nature communications
A primary challenge in single-cell RNA sequencing (scRNA-seq) studies comes from the massive amount of data and the excess noise level. To address this challenge, we introduce an analysis framework, named single-cell Decomposition using Hierarchical ...

A microfluidic robot for rare cell sorting based on machine vision identification and multi-step sorting strategy.

Talanta
The identification, sorting and analysis of rare target single cells in human blood has always been a clinically meaningful medical challenge. Here, we developed a microfluidic robot platform for sorting specific rare cells from complex clinical bloo...

Transfer learning efficiently maps bone marrow cell types from mouse to human using single-cell RNA sequencing.

Communications biology
Biomedical research often involves conducting experiments on model organisms in the anticipation that the biology learnt will transfer to humans. Previous comparative studies of mouse and human tissues were limited by the use of bulk-cell material. H...

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches.

International journal of molecular sciences
Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection ...

Purification of viable peripheral blood mononuclear cells for biobanking using a robotized liquid handling workstation.

Journal of translational medicine
BACKGROUND: The purification of peripheral blood mononuclear cells (PBMCs) by means of density gradient (1.07 g/mL) centrifugation is one of the most commonly used methods in diagnostics and research laboratories as well as in biobanks. Here, we eval...

A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS).

Nature communications
Cell state-specific promoters constitute essential tools for basic research and biotechnology because they activate gene expression only under certain biological conditions. Synthetic Promoters with Enhanced Cell-State Specificity (SPECS) can be supe...

Automation of the Differential Digestion Process of Sexual Assault Evidence.

Journal of forensic sciences
Sexual assault evidence samples require the use of a specific process known as a differential digestion to separate sperm from nonsperm cells prior to DNA extraction. An automated differential digestion process was developed using a selective degrada...

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.

Nature chemical biology
We developed a new way to engineer complex proteins toward multidimensional specifications using a simple, yet scalable, directed evolution strategy. By robotically picking mammalian cells that were identified, under a microscope, as expressing prote...