AIMC Topic: Cell Separation
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Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches.
International journal of molecular sciences
Mar 27, 2020
Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection ...
Purification of viable peripheral blood mononuclear cells for biobanking using a robotized liquid handling workstation.
Journal of translational medicine
Nov 12, 2019
BACKGROUND: The purification of peripheral blood mononuclear cells (PBMCs) by means of density gradient (1.07 g/mL) centrifugation is one of the most commonly used methods in diagnostics and research laboratories as well as in biobanks. Here, we eval...
A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS).
Nature communications
Jun 28, 2019
Cell state-specific promoters constitute essential tools for basic research and biotechnology because they activate gene expression only under certain biological conditions. Synthetic Promoters with Enhanced Cell-State Specificity (SPECS) can be supe...
Automation of the Differential Digestion Process of Sexual Assault Evidence.
Journal of forensic sciences
Jul 25, 2018
Sexual assault evidence samples require the use of a specific process known as a differential digestion to separate sperm from nonsperm cells prior to DNA extraction. An automated differential digestion process was developed using a selective degrada...
A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.
Nature chemical biology
Feb 26, 2018
We developed a new way to engineer complex proteins toward multidimensional specifications using a simple, yet scalable, directed evolution strategy. By robotically picking mammalian cells that were identified, under a microscope, as expressing prote...
Analogous detection of circulating tumor cells using the AccuCyte -CyteFinder system and ISET system in patients with locally advanced and metastatic prostate cancer.
The Prostate
Dec 29, 2017
INTRODUCTION: Circulating tumor cells (CTCs) can provide important information on patient's prognosis and treatment efficacy. Currently, a plethora of methods is available for the detection of these rare cells. We compared the outcomes of two of thos...
Label-free sensor for automatic identification of erythrocytes using digital in-line holographic microscopy and machine learning.
Biosensors & bioelectronics
Dec 15, 2017
Cell types of erythrocytes should be identified because they are closely related to their functionality and viability. Conventional methods for classifying erythrocytes are time consuming and labor intensive. Therefore, an automatic and accurate eryt...
Autofocusing and Polar Body Detection in Automated Cell Manipulation.
IEEE transactions on bio-medical engineering
Jul 13, 2016
Autofocusing and feature detection are two essential processes for performing automated biological cell manipulation tasks. In this paper, we have introduced a technique capable of focusing on a holding pipette and a mammalian cell under a bright-fie...
Automated single cell isolation from suspension with computer vision.
Scientific reports
Feb 9, 2016
Current robots can manipulate only surface-attached cells seriously limiting the fields of their application for single cell handling. We developed a computer vision-based robot applying a motorized microscope and micropipette to recognize and gently...
Challenges in circulating tumor cell detection by the CellSearch system.
Molecular oncology
Dec 25, 2015
Enumeration and characterization of circulating tumor cells (CTC) hold the promise of a real time liquid biopsy. They are however present in a large background of hematopoietic cells making their isolation technically challenging. In 2004, the CellSe...
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