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Enzyme-Linked Immunosorbent Assay

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Analysis and validation of serum biomarkers in brucellosis patients through proteomics and bioinformatics.

Frontiers in cellular and infection microbiology
INTRODUCTION: This study aims to utilize proteomics, bioinformatics, and machine learning algorithms to identify diagnostic biomarkers in the serum of patients with acute and chronic brucellosis.

Evaluation of the effect of cell freshness on pyrogen detection using a serum-free monocyte-activation test.

PloS one
Pyrogens cause shock symptoms when released into the bloodstream. They are classified into two main categories: endotoxins (lipopolysaccharides [LPS]) and non-endotoxin pyrogens. The monocyte activation test (MAT) is an in vitro assay to detect pyrog...

Plasma insulin-like growth factor-II (IGF-II) and IGF-II/IGF-I ratio in a chilean case of Doege-Potter Syndrome.

Revista medica de Chile
INTRODUCTION: Doege-Potter syndrome is a rare clinical entity characterized by recurrent hypoglycemic events caused by non-pancreatic tumors secreting an incompletely processed high-molecular-weight form of Insulin-like Growth factor-II (IGF-II).

IgE anti-BP180 NC16A autoantibody in both serum and blister fluid samples does not correlate with disease activity of bullous pemphigoid.

European journal of dermatology : EJD
The correlation between IgE anti-BP180 NC16A autoantibody and disease activity of bullous pemphigoid (BP) remains disputable. To determine the levels of IgE anti-BP180 NC16A autoantibody and its clinical significance in untreated BP patients. IgG and...

Salivary and serum levels of soluble E-cadherin in patients with gastrointestinal cancers: A comparative study.

Journal of cancer research and therapeutics
AIM: According to the literature, high levels of salivary soluble E-cadherin may be lined to advanced stage and poor prognosis in cancers. This research aimed at comparing salivary and serum levels of soluble E-cadherin in cases with esophageal, gast...

Deep learning-assisted ultra-accurate smartphone testing of paper-based colorimetric ELISA assays.

Analytica chimica acta
Smartphone has long been considered as one excellent platform for disease screening and diagnosis, especially when combined with microfluidic paper-based analytical devices (μPADs) that feature low cost, ease of use, and pump-free operations. In this...

Rapid deep learning-assisted predictive diagnostics for point-of-care testing.

Nature communications
Prominent techniques such as real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and rapid kits are currently being explored to both enhance sensitivity and reduce assay time for diagnostic tests. Existing commerc...

Pretrainable geometric graph neural network for antibody affinity maturation.

Nature communications
Increasing the binding affinity of an antibody to its target antigen is a crucial task in antibody therapeutics development. This paper presents a pretrainable geometric graph neural network, GearBind, and explores its potential in in silico affinity...

TimePAD─Unveiling Temporal Sequence ELISA Signal by Deep Learning for Rapid Readout and Improved Accuracy in a Microfluidic Paper-Based Analytical Platform.

Analytical chemistry
The integration of paper-based microfluidics with deep learning represents a pivotal trend in enhancing diagnostic capabilities. This paper introduces a new approach to improve the performance of a paper-based microfluidic enzyme-linked immunosorbent...