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Immunoassay

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Etching reaction-based photoelectrochemical immunoassay of aflatoxin B in foodstuff using cobalt oxyhydroxide nanosheets-coating cadmium sulfide nanoparticles as the signal tags.

Analytica chimica acta
A new split-type photoelectrochemical (PEC) immunosensing platform was designed for sensitive detection of aflatoxin B (AFB) in foodstuffs, coupling with enzymatic hydrolysate-triggered etching reaction of cobalt oxyhydroxide (CoOOH) on cadmium sulfi...

Dihydropteroate synthase based sensor for screening multi-sulfonamides residue and its comparison with broad-specific antibody based immunoassay by molecular modeling analysis.

Analytica chimica acta
A biosensor that could simultaneously detect multi-analyte as many as in a single test is more favored when facing lots of samples for screening purpose. In such a biosensor, the recognition element with broad specificity and highly affinity is a key...

Paper-based immunocapture for targeted protein analysis.

Talanta
A novel sampling concept for mass spectrometric bottom-up targeted protein analysis is here demonstrated with polymeric sampling spots integrated with instant immunocapture for analysis of dried matrix spots. The polymers 2-hydroxyethyl methacrylate-...

Alarm lateral flow immunoassay for detection of the total infection caused by the five viruses.

Talanta
This study presents new type of the lateral flow immunoassay (LFIA) for multi-target analysis. A test, named alarm-LFIA, has an essentially new function that consists in notice (signaling the danger) about the presence at least one target from the co...

Development of a surface plasmon resonance immunosensor and ELISA for 3-nitrotyrosine in human urine.

Talanta
3-Nitrotyrosine (3-NT) is thought to be a relevant biomarker of nitrosative stress which is associated with many inflammatory and chronic diseases. It is necessary to develop confidential method for specific and sensitive 3-NT detection. In this pape...

Paper-based fluorescent immunoassay for highly sensitive and selective detection of norfloxacin in milk at picogram level.

Talanta
Norfloxacin (NOR) in milk may influence mammalian cell replication and bring about a decrease in the efficiency for treating infection in humans. However, current techniques for detecting NOR usually require expensive instruments and trained personne...

A novel algorithm to improve specificity in ovarian cancer detection.

Cancer treatment and research communications
BACKGROUND: Measurement of autoantibodies (AAbs) to tumor associated antigens has been proposed to aid in the early detection of ovarian cancer with high specificity. Here we describe a multiplex approach to evaluate selected peptide epitopes of p53 ...

[Preparation of monoclonal antibody against cystatin C and establishment of its immunoassay system].

Wei sheng yan jiu = Journal of hygiene research
OBJECTIVE: To prepare human cystatin C( CysC) recombinant protein and produce monoclonal antibodies with high affinity and specificity. Develop a competitive ELISA detection system to detect of CysC in human serum.

Enhancement of lateral flow immunoassay by alkaline phosphatase: a simple and highly sensitive test for potato virus X.

Mikrochimica acta
Alkaline phosphatase (ALP) was used as an amplification tool in lateral flow immunoassay (LFIA). Potato virus Х (PVX) was selected as a target analyte because of its high economic importance. Two conjugates of gold nanoparticles were applied, one wit...

Electrochemical immunosensor for the breast cancer marker CA 15-3 based on the catalytic activity of a CuS/reduced graphene oxide nanocomposite towards the electrooxidation of catechol.

Mikrochimica acta
The authors report on an electrochemical immunosensor for the tumor marker carbohydrate antigen 15-3 (CA15-3). It is based on the use of a composite consisting of reduced graphene oxide (RGO) and copper sulfide (CuS) that was placed on a screen-print...