Most of the proteins locate more than one organelle in a cell. Unmixing the localization patterns of proteins is critical for understanding the protein functions and other vital cellular processes. Herein, non-linear machine learning technique is pro...
The use of fluorescence microscopy has undergone a major revolution over the past twenty years, both with the development of dramatic new technologies and with the widespread adoption of image analysis and machine learning methods. Many open source s...
Application of personalized medicine requires integration of different data to determine each patient's unique clinical constitution. The automated analysis of medical data is a growing field where different machine learning techniques are used to mi...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2025
Fluorescence microscopy is a key method for the visualization of cellular, subcellular, and molecular live-cell dynamics, enabling access to novel insights into mechanisms of health and disease. However, effects like phototoxicity, the fugitive natur...
The accuracy of assigning fluorophore identity and abundance, known as spectral unmixing, in biological fluorescence microscopy images remains a significant challenge due to the substantial overlap in emission spectra among fluorophores. In tradition...
Zhonghua nan ke xue = National journal of andrology
Oct 1, 2023
OBJECTIVE: To study the correlation, consistency, and variations between two assays of DNA fragmentation index based on acridine orange (AO) staining via AI-based fluorescence microscopy(AI-DFI), and flow cytometry (FCM-DFI) across multiple centers.
Intravital microscopy in small animals growingly contributes to the visualization of short- and long-term mammalian biological processes. Miniaturized fluorescence microscopy has revolutionized the observation of live animals' neural circuits. The te...
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Jul 1, 2023
Accurate single cell segmentation provides means to monitor the behavior of single cell within a population of cells. Time-lapse fluorescence images are used to reveal heterogeneous nature of single mouse embryonic stem cell (ESC) colony and monitor ...
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Jul 1, 2023
Miniaturized fluorescence microscopy has revolutionized the way neuroscientists study the brain in-vivo. Recent developments in computational lensless imaging promise a next generation of miniaturized microscopes in lensless fluorescence microscopy. ...
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Feb 25, 2023
This paper reviews the research progress on live-cell super-resolution fluorescence microscopy, discusses the current research status and hotspots in this field, and summarizes the technological application of super-resolution fluorescence microscopy...