AI Medical Compendium Topic

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Single-Cell Analysis

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Using transfer learning from prior reference knowledge to improve the clustering of single-cell RNA-Seq data.

Scientific reports
In many research areas scientists are interested in clustering objects within small datasets while making use of prior knowledge from large reference datasets. We propose a method to apply the machine learning concept of transfer learning to unsuperv...

A tri-functional probe mediated exponential amplification strategy for highly sensitive detection of Dnmt1 and UDG activities at single-cell level.

Analytica chimica acta
Multiplex DNA methylation and glycosylation are ubiquitous in the human body to ensure the normal function and stability of the genome. The methyltransferases and glycosylases rely on varied enzymes with different action mechanism, which still remain...

Single-cell ATAC-Seq in human pancreatic islets and deep learning upscaling of rare cells reveals cell-specific type 2 diabetes regulatory signatures.

Molecular metabolism
OBJECTIVE: Type 2 diabetes (T2D) is a complex disease characterized by pancreatic islet dysfunction, insulin resistance, and disruption of blood glucose levels. Genome-wide association studies (GWAS) have identified > 400 independent signals that enc...

DeepImpute: an accurate, fast, and scalable deep neural network method to impute single-cell RNA-seq data.

Genome biology
Single-cell RNA sequencing (scRNA-seq) offers new opportunities to study gene expression of tens of thousands of single cells simultaneously. We present DeepImpute, a deep neural network-based imputation algorithm that uses dropout layers and loss fu...

Exploring single-cell data with deep multitasking neural networks.

Nature methods
It is currently challenging to analyze single-cell data consisting of many cells and samples, and to address variations arising from batch effects and different sample preparations. For this purpose, we present SAUCIE, a deep neural network that comb...

Pathway-Based Single-Cell RNA-Seq Classification, Clustering, and Construction of Gene-Gene Interactions Networks Using Random Forests.

IEEE journal of biomedical and health informatics
Single-cell RNA-Sequencing (scRNA-Seq), an advanced sequencing technique, enables biomedical researchers to characterize cell-specific gene expression profiles. Although studies have adapted machine learning algorithms to cluster different cell popul...

Towards early monitoring of chemotherapy-induced drug resistance based on single cell metabolomics: Combining single-probe mass spectrometry with machine learning.

Analytica chimica acta
Despite the presence of methods evaluating drug resistance during chemotherapies, techniques, which allow for monitoring the degree of drug resistance in early chemotherapeutic stage from single cells in their native microenvironment, are still absen...

Cell Type Classification and Unsupervised Morphological Phenotyping From Low-Resolution Images Using Deep Learning.

Scientific reports
Convolutional neural networks (ConvNets) have proven to be successful in both the classification and semantic segmentation of cell images. Here we establish a method for cell type classification utilizing images taken with a benchtop microscope direc...

Learning unsupervised feature representations for single cell microscopy images with paired cell inpainting.

PLoS computational biology
Cellular microscopy images contain rich insights about biology. To extract this information, researchers use features, or measurements of the patterns of interest in the images. Here, we introduce a convolutional neural network (CNN) to automatically...