AIMC Topic: Real-Time Polymerase Chain Reaction
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Verification and large scale clinical evaluation of a national standard protocol for Salmonella spp./Shigella spp. screening using real-time PCR combined with guided culture.
Journal of microbiological methods
Dec 17, 2017
Salmonella spp./Shigella spp. are often associated with food poisoning and fecal-oral transmission of acute gastroenteritis that requires strict monitoring, especially among people who would handle food and water. In 2014, the National Health and Fam...
Effects and inhibition mechanism of phenazine-1-carboxamide on the mycelial morphology and ultrastructure of Rhizoctonia solani.
Pesticide biochemistry and physiology
Oct 24, 2017
The purpose of this research was to explore the effect of phenazine-1-carboxamide (PCN) on Rhizoctonia solani and to elucidate its mechanisms of action. The toxicity of PCN to R. solani was measured using a growth rate method. The results indicated t...
An aptamer-based effective method for highly sensitive detection of chloramphenicol residues in animal-sourced food using real-time fluorescent quantitative PCR.
Talanta
Dec 30, 2016
Chloramphenicol (CAP) residues can not only harm human health through entering food chain, but also cause the spreading of drug-resistant bacteria, thereby leading to secondary environmental pollution. Therefore, it is in urgent need of establishing ...
Identification of Factors for the Preoperative Prediction of Tumour Subtype and Prognosis in Patients with T1 Lung Adenocarcinoma.
Disease markers
Dec 26, 2016
Identification of factors that can predict the subtypes of lung adenocarcinoma preoperatively is important for selecting the appropriate surgical procedure and for predicting postoperative survival. We retrospectively evaluated 87 patients with lun...
Carcinoembryonic Antigen
Preoperative Care
Follow-Up Studies
Neoplasm Invasiveness
MicroRNAs
Neoplasm Staging
Survival Rate
Biomarkers, Tumor
Male
Middle Aged
Lung Neoplasms
RNA, Messenger
Adenocarcinoma
Female
Real-Time Polymerase Chain Reaction
Aged
Reverse Transcriptase Polymerase Chain Reaction
Aged, 80 and over
Humans
Adult
Prognosis
Retrospective Studies
Tomography, X-Ray Computed
Identification of DEP domain-containing proteins by a machine learning method and experimental analysis of their expression in human HCC tissues.
Scientific reports
Dec 21, 2016
The Dishevelled/EGL-10/Pleckstrin (DEP) domain-containing (DEPDC) proteins have seven members. However, whether this superfamily can be distinguished from other proteins based only on the amino acid sequences, remains unknown. Here, we describe a com...
Gene Expression Profiling
Real-Time Polymerase Chain Reaction
Liver Neoplasms
Carcinoma, Hepatocellular
Humans
Cluster Analysis
Machine Learning
Protein Domains
Algorithms
Phylogeny
Amino Acid Motifs
GTPase-Activating Proteins
Computational Biology
Dishevelled Proteins
RNA, Messenger
Gene Expression Regulation, Neoplastic
Signal Transduction
Persistence of influenza on surfaces.
The Journal of hospital infection
Dec 13, 2016
BACKGROUND: Close contact transmission (either direct or large droplet/droplet nuclei) is considered the main driver of influenza outbreaks but there is limited information regarding the role of fomites in transmission.
Designing primers and evaluation of the efficiency of propidium monoazide - Quantitative polymerase chain reaction for counting the viable cells of Lactobacillus gasseri and Lactobacillus salivarius.
Journal of food and drug analysis
Nov 29, 2016
The purpose of this study is to evaluate the efficiency of using propidium monoazide (PMA) real-time quantitative polymerase chain reaction (qPCR) to count the viable cells of Lactobacillus gasseri and Lactobacillus salivarius in probiotic products. ...
Transcriptomic alterations in Sitophilus zeamais in response to allyl isothiocyanate fumigation.
Pesticide biochemistry and physiology
Oct 11, 2016
To study the fumigation mechanisms of Allyl isothiocyanate (AITC) a promising biorational alternative to present fumigants (phosphine and methyl bromide), and provide theoretical basis for its further development in the control of stored grain pests,...
Artificial Intelligence for the Evaluation of Operational Parameters Influencing Nitrification and Nitrifiers in an Activated Sludge Process.
Microbial ecology
Feb 23, 2016
Nitrification at a full-scale activated sludge plant treating municipal wastewater was monitored over a period of 237 days. A combination of fluorescent in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (qPCR) were use...
Heat shock protein A12B protects against sepsis-induced impairment in vascular endothelial permeability.
The Journal of surgical research
Dec 30, 2015
BACKGROUND: As a common and life-threatening infectious syndrome, sepsis contributes significantly to morbidity and mortality in clinical settings. Vascular endothelial injury and hyperpermeability play an important role in the development of sepsis-...
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