AIMC Topic: Blotting, Western

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Metformin Alleviates Lipopolysaccharide-induced Acute Lung Injury through Suppressing Toll-like Receptor 4 Signaling.

Iranian journal of allergy, asthma, and immunology Dec 1, 2016
Signaling AMP-activated protein kinase (AMPK), an energy sensing enzyme, has been implicated in controlling inflammation. In this study we investigated whether activation of AMPK by metformin could protect the lung from lipopolysaccharide (LPS)-induc...
Hypoglycemic Agents Peroxidase Lipopolysaccharides Acute Lung Injury Tumor Necrosis Factor-alpha Blotting, Western Real-Time Polymerase Chain Reaction Animals NF-kappa B Male RNA, Messenger AMP-Activated Protein Kinases Lung Rats, Wistar Metformin Rats Myeloid Differentiation Factor 88 Toll-Like Receptor 4
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Intraoperative Diagnosis Support Tool for Serous Ovarian Tumors Based on Microarray Data Using Multicategory Machine Learning.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society Jan 1, 2016
OBJECTIVES: Serous borderline ovarian tumors (SBOTs) are a subtype of serous ovarian carcinoma with atypical proliferation. Frozen-section diagnosis has been used as an intraoperative diagnosis tool in supporting the fertility-sparing surgery by diag...
Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Neoplasm Staging Databases, Genetic Gene Expression Regulation, Neoplastic Biomarkers, Tumor Carcinoma, Ovarian Epithelial Ovarian Neoplasms Prognosis Support Vector Machine Gene Expression Profiling Predictive Value of Tests Immunoenzyme Techniques Female Monitoring, Intraoperative Neoplasms, Glandular and Epithelial Blotting, Western Humans Survival Rate Real-Time Polymerase Chain Reaction Machine Learning Cystadenocarcinoma, Serous
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[Study of FN1 and ZNF438 gene expression negatively regulated by androgen receptor in androgen-independent LNCaP cells].

Zhonghua yi xue za zhi Dec 19, 2015
OBJECTIVE: To verify whether FN1 and ZNF438 are the androgen receptor(AR) target genes in LNCaP-AI cells and investigate the effects of AR exerts on FN1 and ZNF438 gene expression.
RNA, Messenger Gene Expression Regulation, Neoplastic Gene Expression Blotting, Western Down-Regulation Receptors, Androgen Oligonucleotide Array Sequence Analysis Prostatic Neoplasms Fibronectins Repressor Proteins Humans Androgens Male Dihydrotestosterone Cell Line, Tumor
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Astragalus Polysaccharide Inhibits Autophagy and Apoptosis from Peroxide-Induced Injury in C2C12 Myoblasts.

Cell biochemistry and biophysics Nov 1, 2015
The aim is to study the effects and underlying mechanisms of astragalus polysaccharide (APS) on the peroxide-induced injury in C2C12 myoblasts in vitro. Cell viability in the presence or absence of APS was detected by the methyl thiazolyl tetrazolium...
Ribosomal Protein S6 Kinases, 70-kDa Microscopy, Electron Microtubule-Associated Proteins Mice Astragalus Plant Cell Survival TOR Serine-Threonine Kinases Signal Transduction Animals Cytochromes c Caspase 3 bcl-2-Associated X Protein Apoptosis Hydrogen Peroxide Blotting, Western Polysaccharides Cell Line Autophagy Proto-Oncogene Proteins c-bcl-2 Myoblasts
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[Construction of Recombinant Adenovirus Vector with Human/Mouse Interferon Gene and Its Transfection into Human Umbilical Cord Mesenchymal Stem Cells].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition Nov 1, 2015
OBJECTIVE: Construction and identification of recombinant adenovirus vector with human/mouse interferon (IFN-gamma) and effectively transfection into human umbilical cord mesenchymal stem cells (HUMSCs).
Humans Animals Mice Enzyme-Linked Immunosorbent Assay Green Fluorescent Proteins HEK293 Cells Blotting, Western Interferons Plasmids Microscopy, Fluorescence Adenoviridae Polymerase Chain Reaction Mesenchymal Stem Cells Genetic Vectors Umbilical Cord Transfection
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Effect of Mast Cell Chymase on Activation, Proliferation and Transdifferentiation of Hepatic Stellate Cells.

Hepato-gastroenterology Jun 1, 2015
BACKGROUND/AIMS: This work aims to observe the effects of mast cell (MC) chymase on activation, proliferation and transdifferentiation of hepatic stellate cells (HSCs) and to discuss the role of chymase in development of liver fibrosis.
Cell Transdifferentiation Chymases Rats, Wistar Rats Animals Hepatic Stellate Cells Cell Proliferation Blotting, Western Male Actins Mast Cells Transforming Growth Factor beta1 Real-Time Polymerase Chain Reaction Cells, Cultured Collagen Type I RNA, Messenger
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Doxorubicin enhances (131)I-rituximab induced cell death in Raji cells.

Journal of cancer research and therapeutics Jan 1, 2015
AIM: There are various therapeutic modalities of treatment for non-Hodgkin's lymphoma, but with certain limitations, hence, investigating the scope of combined therapeutic approach.
Poly(ADP-ribose) Polymerases Burkitt Lymphoma Radiation-Sensitizing Agents Blotting, Western Flow Cytometry Radiopharmaceuticals Doxorubicin Iodine Radioisotopes Drug Synergism Tumor Cells, Cultured Rituximab Cell Proliferation Signal Transduction Antineoplastic Combined Chemotherapy Protocols Apoptosis Humans RNA, Messenger
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Regulation of demethylation and re-expression of RASSF1A gene in hepatocellular carcinoma cell lines treated with NCTD in vitro.

Journal of cancer research and therapeutics Jan 1, 2015
BACKGROUND: Hepatocellular carcinoma, a lethal malignant neoplasm with poor prognosis, has dismal results of surgical resection and chemoradiotherapy. Norcantharidin (NCTD), the demethylated analog of cantharidin derived from a traditional Chinese me...
In Vitro Techniques Liver Neoplasms Carcinoma, Hepatocellular Gene Expression Regulation, Neoplastic Humans Tumor Cells, Cultured DNA Methylation Blotting, Western Tumor Suppressor Proteins Bridged Bicyclo Compounds, Heterocyclic
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