AIMC Topic: Coculture Techniques
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Aeromonas hydrophila biofilm, exoprotease, and quorum sensing responses to co-cultivation with diverse foodborne pathogens and food spoilage bacteria on crab surfaces.
Biofouling
Jan 30, 2019
The effects of dual species interactions on biofilm formation by Aeromonas hydrophila in the presence of Pseudomonas aeruginosa, Pseudomonas fluorescens, Pectobacterium carotovorum, Salmonella Typhimurium, and Listeria monocytogenes were examined. Hi...
Morphological profiling using machine learning reveals emergent subpopulations of interferon-γ-stimulated mesenchymal stromal cells that predict immunosuppression.
Cytotherapy
Nov 28, 2018
BACKGROUND: Although a preponderance of pre-clinical data demonstrates the immunosuppressive potential of mesenchymal stromal cells (MSCs), significant heterogeneity and lack of critical quality attributes (CQAs) based on immunosuppressive capacity l...
Production of lipid-containing algal-bacterial polyculture in wastewater and biomethanation of lipid extracted residues: Enhancing methane yield through hydrothermal pretreatment and relieving solvent toxicity through co-digestion.
The Science of the total environment
Nov 6, 2018
The feasibility of generating a lipid-containing algal-bacterial polyculture biomass in municipal primary wastewater and enhancing biomethanation of lipid-extracted algal residues (LEA) through hydrothermal pretreatment and co-digestion with sewage s...
In vitro growth and maturation of isolated caprine preantral follicles: Influence of insulin and FSH concentration, culture dish, coculture, and oocyte size on meiotic resumption.
Theriogenology
Nov 9, 2016
The aims of this study were: (1) to evaluate the effect of different insulin concentrations, alone or in combination with either a fixed FSH concentration or increasing FSH concentrations on the in vitro culture of isolated caprine preantral follicle...
Multivariate Calibration Approach for Quantitative Determination of Cell-Line Cross Contamination by Intact Cell Mass Spectrometry and Artificial Neural Networks.
PloS one
Jan 28, 2016
Cross-contamination of eukaryotic cell lines used in biomedical research represents a highly relevant problem. Analysis of repetitive DNA sequences, such as Short Tandem Repeats (STR), or Simple Sequence Repeats (SSR), is a widely accepted, simple, a...
Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts.
BioMed research international
Dec 22, 2015
INTRODUCTION: Human mesenchymal stromal cells (hMSCs) exhibit the potential to accelerate bone healing by enhanced osteogenic differentiation. Interleukin-1β is highly expressed during fracture healing and has been demonstrated to exert a significant...
Quantifying co-cultured cell phenotypes in high-throughput using pixel-based classification.
Methods (San Diego, Calif.)
Dec 11, 2015
Biologists increasingly use co-culture systems in which two or more cell types are grown in cell culture together in order to better model cells' native microenvironments. Co-cultures are often required for cell survival or proliferation, or to maint...
Effect of Cell-Cell Interaction on Single-Cell Behavior Revealed by a Deep Learning-Aided High-Throughput Addressable Single-Cell Coculture System.
Analytical chemistry
May 13, 2025
Cell-cell interactions are crucial for understanding various physiological and pathological processes, yet conventional population-level methods fail to disclose the heterogeneity at a single-cell resolution. Single-cell coculture systems that isolat...
Application of an artificial intelligence for quantitative analysis of endothelial capillary beds in vitro.
Clinical hemorheology and microcirculation
Jan 1, 2024
BACKGROUND: The use of endothelial cell cultures has become fundamental to study angiogenesis. Recent advances in artificial intelligences (AI) offer opportunities to develop automated assessment methods in medical research, analyzing larger datasets...
Barcodes, co-cultures, and deep learning take genetically encoded biosensor multiplexing to the nth degree.
Molecular cell
Jan 20, 2022
Yang et al. (2021) describe a co-culture multiplexed imaging method that can provide an order of magnitude increase in the number of barcoded biosensors that can be imaged in a single experiment.
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