AIMC Topic: Enzyme-Linked Immunosorbent Assay
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Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel.
Phytochemical analysis : PCA
Dec 20, 2017
INTRODUCTION: Paclitaxel (Tax) is a diterpene alkaloid isolated from Taxus species and has proved clinically effective in treating a number of malignancies. Current quantitative analytical methods for Tax such as high-performance liquid chromatograph...
The ability to predict pregnancy loss in cattle with ELISAs that detect pregnancy associated glycoproteins is antibody dependent.
Theriogenology
Dec 14, 2017
The concentration of circulating pregnancy associated glycoproteins (PAGs) early in pregnancy may serve as markers to predict late embryonic mortality or fetal mortality in cattle. In this study, pregnancies were established in dairy cows, by either ...
Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
Dec 11, 2017
Ginsenoside Rg1, one of the most notable active components of Panax ginseng, has been widely reported to exert anti-inflammatory actions. This study aimed to reveal whether ginsenoside Rg1 also exhibits beneficial roles against lipopolysaccharide (LP...
Blotting, Western
Anti-Inflammatory Agents
Phosphatidylinositol 3-Kinases
Proto-Oncogene Proteins c-akt
Humans
Analysis of Variance
Nephritis
Kidney Tubules
Cytokines
Reproducibility of Results
Cell Migration Assays
Enzyme-Linked Immunosorbent Assay
Ginsenosides
Apoptosis
Lipopolysaccharides
Cell Line
Reactive Oxygen Species
Epithelial Cells
Cell Survival
Protective Agents
Nanoparticle based bio-bar code technology for trace analysis of aflatoxin B1 in Chinese herbs.
Journal of food and drug analysis
Dec 6, 2017
A novel and sensitive assay for aflatoxin B1 (AFB1) detection has been developed by using bio-bar code assay (BCA). The method that relies on polyclonal antibodies encoded with DNA modified gold nanoparticle (NP) and monoclonal antibodies modified ma...
Universal simultaneous multiplex ELISA of small molecules in milk based on dual luciferases.
Analytica chimica acta
Nov 22, 2017
The enzyme-linked immunosorbent assay (ELISA) has become the most important and widely used rapid detection technology for food safety because of its simple operation, fast speed and high sensitivity. Multiplex synchronous detection is the goal of EL...
Development of a highly sensitive and specific ELISA method for the determination of l-corydalmine in SD rats with monoclonal antibody.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Sep 14, 2017
l-Corydalmine (l-CDL) is a potent analgesic constituent of the traditional Chinese medicine, Rhizoma Corydalis. However, the pharmacokinetic process and tissue distribution of l-CDL in vivo are still unknown. Therefore, it is necessary to establish a...
The Essential Oils of Hairy Roots and Roots of Soil-Grown Plants: Chemical Composition and Antimicrobial, Anti-Inflammatory, and Antioxidant Activities.
Oxidative medicine and cellular longevity
Dec 18, 2016
The essential oils were isolated by hydrodistillation from the hairy roots (HR) and roots of soil-grown plants (SGR) of and were analyzed by GC-MS method. In the both essential oils 62 compounds were identified. The root essential oils showed the di...
Lipopolysaccharides
Cell Line
Humans
Reactive Oxygen Species
Anti-Infective Agents
Microbial Sensitivity Tests
Gas Chromatography-Mass Spectrometry
Plant Roots
Tumor Necrosis Factor-alpha
Oils, Volatile
Enterococcus faecalis
Interleukin-6
Enzyme-Linked Immunosorbent Assay
Saccharomyces cerevisiae
Interleukin-1beta
Candida albicans
Leuzea
Astrocytes
Anti-Inflammatory Agents
Pseudomonas aeruginosa
Optimization of an Anti-poly(ethylene glycol) (anti-PEG) Cell-Based Capture System To Quantify PEG and PEGylated Molecules.
Analytical chemistry
Nov 30, 2016
Sensitive determination of the pharmacokinetics of PEGylated molecules can accelerate the process of drug development. Here, we combined different anti-PEG Fab expressing 293T cells as capture cells (293T/3.3, 293T/6.3, and 293T/15-2b cells) with fou...
Nanobody-based enzyme immunoassay for ochratoxin A in cereal with high resistance to matrix interference.
Talanta
Nov 19, 2016
A sensitive indirect competitive nanobody-based enzyme linked immunosorbent assay (Nb-ELISA) for ochratoxin A (OTA) with high resistance to cereal matrix interference was developed. Nanobodies against OTA (Nb15, Nb28, Nb32, Nb36) were expressed in E....
Investigation of serum Ki-67 as a biomarker in tumor-bearing dogs.
Research in veterinary science
Oct 26, 2016
Because of the limited number of tumor markers in veterinary medicine, there is need for identifying new markers. Ki-67 has been investigated as a tissue marker of malignant alterations. We hypothesized that Ki-67 would also be measurable in serum an...
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