AIMC Topic: Enzyme-Linked Immunosorbent Assay

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HCV nonstructural protein 4 is associated with aggressiveness features of breast cancer.

Breast cancer (Tokyo, Japan)
BACKGROUND: Hepatitis C virus (HCV) has the lymphotropic feature that is supposed to be the reason of related extrahepatic manifestation. HCV viral oncoproteins may participate in the regulation of some gene expression that has been implicated in tum...

Synergistic Use of Gold Nanoparticles (AuNPs) and "Capillary Enzyme-Linked Immunosorbent Assay (ELISA)" for High Sensitivity and Fast Assays.

Sensors (Basel, Switzerland)
Using gold nanoparticles (AuNPs) on "capillary enzyme-linked immunosorbent assay (ELISA)", we produced highly sensitive and rapid assays, which are the major attributes for point-of-care applications. First, in order to understand the size effect of ...

Antigenic and pathogenicity activities of Ralstonia solanacearum race 3 biovar 2 molecularly identified and detected by indirect ELISA using polyclonal antibodies generated in rabbits.

Microbial pathogenesis
Eight molecular-characterized isolates of Ralstonia solanacearum from potato belonging to race 3 biovar 2, their virulence were evaluated on potato cv. Lady Rosette, tomato cv. Strain B, eggplant cv. Balady and pepper cv. Balady and showed high virul...

Development of a Competitive Time-Resolved Fluoroimmunoassay for Paclitaxel.

Phytochemical analysis : PCA
INTRODUCTION: Paclitaxel (Tax) is a diterpene alkaloid isolated from Taxus species and has proved clinically effective in treating a number of malignancies. Current quantitative analytical methods for Tax such as high-performance liquid chromatograph...

The ability to predict pregnancy loss in cattle with ELISAs that detect pregnancy associated glycoproteins is antibody dependent.

Theriogenology
The concentration of circulating pregnancy associated glycoproteins (PAGs) early in pregnancy may serve as markers to predict late embryonic mortality or fetal mortality in cattle. In this study, pregnancies were established in dairy cows, by either ...

Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
Ginsenoside Rg1, one of the most notable active components of Panax ginseng, has been widely reported to exert anti-inflammatory actions. This study aimed to reveal whether ginsenoside Rg1 also exhibits beneficial roles against lipopolysaccharide (LP...

Nanoparticle based bio-bar code technology for trace analysis of aflatoxin B1 in Chinese herbs.

Journal of food and drug analysis
A novel and sensitive assay for aflatoxin B1 (AFB1) detection has been developed by using bio-bar code assay (BCA). The method that relies on polyclonal antibodies encoded with DNA modified gold nanoparticle (NP) and monoclonal antibodies modified ma...

Universal simultaneous multiplex ELISA of small molecules in milk based on dual luciferases.

Analytica chimica acta
The enzyme-linked immunosorbent assay (ELISA) has become the most important and widely used rapid detection technology for food safety because of its simple operation, fast speed and high sensitivity. Multiplex synchronous detection is the goal of EL...

Development of a highly sensitive and specific ELISA method for the determination of l-corydalmine in SD rats with monoclonal antibody.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
l-Corydalmine (l-CDL) is a potent analgesic constituent of the traditional Chinese medicine, Rhizoma Corydalis. However, the pharmacokinetic process and tissue distribution of l-CDL in vivo are still unknown. Therefore, it is necessary to establish a...